Multiplex detection of Ehrlichia and Anaplasma species pathogens in peripheral blood by real-time reverse transcriptase-polymerase chain reaction

Journal of Molecular Diagnostics

Volumn 7, Issue 2, 2005, Pages 308-316

  Sirigireddy, Kamesh R ^a   Ganta, Roman R ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; RNA 16S;

ANAPLASMA; ANAPLASMA PHAGOCYTOPHILUM; ANAPLASMOSIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIUM DETECTION; BLOOD ANALYSIS; BLOOD SAMPLING; CLINICAL PROTOCOL; COMORBIDITY; CONTROLLED STUDY; DISEASE MODEL; DOG; EHRLICHIA; EHRLICHIA CANIS; EHRLICHIA CHAFFEENSIS; EHRLICHIOSIS; MICROORGANISM DETECTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PATIENT MONITORING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; SPECIES DIFFERENCE; TICK; VERTEBRATE;

ACARI; ANAPLASMA; ANAPLASMA PHAGOCYTOPHILUM; ANAPLASMA PLATYS; CANIS FAMILIARIS; EHRLICHIA; EHRLICHIA CANIS; EHRLICHIA CHAFFEENSIS; EHRLICHIA EWINGII; VERTEBRATA;

EID: 18744404860     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-1578(10)60559-4     Document Type: Article

References (49)

1. Dumler JS, Barbet AF, Bekker CP, Desch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR: Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 2001, 51:2145-2165
2. Dawson JE, Anderson BE, Fishbein DB, Sanchez CY, Goldsmith CY, Wilson KH, Duntley CW: Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis. J Clin Microbiol 1991, 29:2741-2745
3. Maeda K, Markowitz N, Hawley RC, Ristic M, Cox D, McDade JE: Human infection with Ehrlichia canis, a leukocytic rickettsia. N Engl J Med 1987, 316:853-856
4. Chen SM, Dumler JS, Bakken JS, Walker DH: Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol 1994, 32:589-595
5. Buller RS, Arens M, Hmiel SP, Paddock CD, Sumner JW, Rikhisa Y, Unver A, Gaudreault-Keener M, Manian FA, Liddell AM, Schmulewitz N, Storch GA: Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. N Engl J Med 1999, 341:148-155
6. Breitschwerdt EB, Hegarty BC, Hancock SI: Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii. J Clin Microbiol 1998, 36:2645-2651
7. French TW, Harvey JW: Canine infectious cyclic thrombocytopenia (Ehrlichia platys infection in dogs). Rickettsial and Chlamydial Diseases of Domestic Animals. Edited by Woldehiwet Z, Ristic M. Oxford, Pergamon Press, 1993, pp 195-208
8. Murphy GL, Ewing SA, Whitworth LC, Fox JC, Kocan AA: A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Vet Parasitol 1998, 79:325-339
9. Paddock CD, Childs JE: Ehrlichia chaffeensis: a prototypical emerging pathogen. Clin Microbiol Rev 2003, 16:37-64
10. Perez M, Rikihisa Y, Wen B: Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J Clin Microbiol 1996, 34:2133-2139
11. Kordick SK, Breitschwerdt EB, Hegarty BC, Southwick KL, Colitz CM, Hancock SI, Bradley JM, Rumbough R, Mcpherson JT, MacCormack JN: Coinfection with multiple tick-borne pathogens in a Walker Hound kennel in North Carolina. J Clin Microbiol 1999, 37:2631-2638
12. Hoskins JD, Breitschwerdt EB, Gaunt SD, French TW, Burgdorfer W: Antibodies to Ehrlichia canis, Ehrlichia platys, and spotted fever group rickettsiae in Louisiana dogs. J Vet Intern Med 1988, 2:55-59
13. Hua P, Yuhai M, Shide T, Yang S, Bohai W, Xiangrui C: Canine ehrlichiosis caused simultaneously by Ehrlichia canis and Ehrlichia platys. Microbiol Immunol 2000, 44:737-739
14. Chang YF, Novosel V, Chang CF, Kim JB, Shin SJ, Lein DH: Detection of human granulocytic ehrlichiosis agent and Borrelia burgdorferi in ticks by polymerase chain reaction. J Vet Diagn Invest 1998, 10:56-59
15. Sexton DJ, Corey GR, Carpenter C, Kong LQ, Gandhi T, Breitschwerdt E, Hegarty B, Chen SM, Feng HM, Yu XJ, Olano J, Walker DH, Dumler SJ: Dual infection with Ehrlichia chaffeensis and a spotted fever group rickettsia: a case report. Emerg Infect Dis 1998, 4:311-316
16. Paddock CD, Folk SM, Shore GM, Machado LJ, Huycke MM, Slater LN, Liddell AM, Buller RS, Storch GA, Monson TP, Rimland D, Sumner JW, Singleton J, Bloch KC, Tang YW, Standaert SM, Childs JE: Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in persons coinfected with human immunodeficiency virus. Clin Infect Dis 2001, 33:1586-1594
17. Meinkoth JH, Ewing SA, Cowell RL, Dawson JE, Warner CK, Mathew JS, Bowles M, Thiessen AE, Panciera RJ, Fox C: Morphologic and molecular evidence of a dual species ehrlichial infection in a dog presenting with inflammatory central nervous system disease. J Vet Intern Med 1998, 12:389-393
18. Codner EC, Farris-Smith LL: Characterization of the sub-clinical phase of ehrlichiosis in dogs. J Am Vet Med Assoc 1986, 189:47-50
19. Dumler JS, Sutker WL, Walker DH: Persistent infection with Ehrlichia chaffeensis. Clin Infect Dis 1993, 17:903-905
20. Egenvall A, Lilliehook I, Bjoersdorff A, Engvall EO, Karlstam E, Artursson K, Heldtander M, Gunnarsson A: Detection of granulocytic Ehrlichia species DNA by PCR in persistently infected dogs. Vet Rec 2000, 146:186-190
21. Kieser ST, Eriks IS, Palmer GH: Cyclic Rickettsemia during persistent Anaplasma marginale infection of cattle. Infect Immun 1990, 58:1117-1119
22. Chen SM, Popov VL, Feng HM, Walker DH: Analysis and ultrastructural localization of Ehrlichia chaffeensis proteins with monoclonal antibodies. Am J Trop Med Hyg 1996, 54:405-412
23. Reddy GR, Sulsona CR, Barbet AF, Mahan SM, Burridge MJ, Alleman AR: Molecular characterization of a 28 kDa surface antigen gene family of the tribe Ehrlichiae. Biochem Biophys Res Commun 1998, 247:636-643
24. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Cold Spring Harbor Laboratory, 1982
25. Devereux J: Genetics computer group sequence analysis software package, version 6.1. Nucleic Acids Res 1984, 12:387-395
26. Stefano JE, Genovese L, An Q, Lu L, McCarty J, Du Y, Stefano K, Burg JL, King W, Lane DJ: Rapid and sensitive detection of Chlamydia trachomatis using a ligatable binary RNA probe and Q beta replicase. Mol Cell Probes 1997, 11:407-426
27. Mackay IM: Real-time PCR in the microbiology laboratory. Clin Microbiol Infect 2004, 10:190-212
28. Szollosi J, Damjanovich S. Matyus L: Application of fluorescence resonance energy transfer in the clinical laboratory: routine and research. Cytometry 1998, 34:159-179
29. Waner T, Harrus S, Jongejan F, Bark H, Keysary A, Cornelissen AW: Significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by Ehrlichia canis. Vet Parasitol 2001, 95:1-15
30. Felek S, Unver A, Stich RW, Rikihisa Y: Sensitive detection of Ehrlichia chaffeensis in cell culture, blood, and tick specimens by reverse transcription-PCR. J Clin Microbiol 2003, 39:460-463
31. Lane RS, Foley JE, Eisen L, Lennette ET, Peot MA: Acarologic risk of exposure to emerging tick-borne bacterial pathogens in a semi-rural community in northern California. Vector Borne Zoonotic Dis 2001, 1:197-210
32. Warner CK, Dawson JE: Genus and species level identification of Ehrlichia species by PCR and sequencing. PCR protocols for emerging infectious diseases. Edited by Persing DH. Washington DC, ASM Press, 1996, pp 100-105
33. Goodman RA, Hawkins EC, Olby NJ, Grindem CB, Hegarty B, Breitschwerdt EB: Molecular identification of Ehrlichia ewingii infection in dogs: 15 cases (1997-2001). J Am Vet Med Assoc 2003, 222:1102-1107
34. McBride JW, Corstvet RE, Gaunt SD, Chinsangaram J, Akita GY, Osburn BI: PCR detection of acute Ehrlichia canis infection in dogs. J Vet Diagn Invest 1996, 8:441-447
35. Stich RW, Rikihisa Y, Ewing SA, Needham GR, Grover DL, Jittapalapong S: Detection of Ehrlichia canis in canine carrier blood and in individual experimentally infected ticks with a p30-based PCR assay. J Clin Microbiol 2002, 40:540-546
36. Engvall EO, Pettersson B, Persson M, Artursson K, Johansson KE: A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses, and cattle. J Clin Microbiol 1996, 34:2170-2174
37. Chang WL, Pan MJ: Specific amplification of Ehrlichia platys DNA from blood specimens by two-step PCR. J Clin Microbiol 1996, 34:3142-3146
38. Arens MQ, Liddell AM, Buening G, Gaudreault-Keener M, Sumner JW, Comer JA, Buller RS, Storch GA: Detection of Ehrlichia spp. in the blood of wild white-tailed deer in Missouri by PCR assay and serologic analysis. J Clin Microbiol 2003, 41:1263-1265
39. Dumler JS: Molecular methods for ehrlichiosis and Lyme disease. Clin Lab Med 2003, 23:867-884
40. Sparagano OA, de VA, Paoletti B, Camma C, de Vos AP, Otranto D, Glangaspero A: Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. J Vet Diagn Invest 2003, 15:527-534
41. Bekker CP, de Vos S, Taoufik A, Sparagano OA, Jongejan F: Simultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridization. Vet Microbiol 2002, 89:223-238
42. Pusterla N, Huder JB, Leutenegger CM, Braun U, Madigan JE, Lutz H: Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks. J Clin Microbiol 1999, 37:1329-1331
43. Loftis AD, Massung RF, Levin ML: Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis. J Clin Microbiol 2003, 41:3870-3872
44. Brouqui P, Salvo E, Dumler JS, Raoult D: Diagnosis of granulocytic ehrlichiosis in humans by immunofluorescence assay. Clin Diagn Lab Immunol 2001, 8:199-202
45. Dawson J, Fishbein D, Eng T, Redus M, Greene N: Diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity. Ann NY Acad Sci 1990, 590:308
46. French TW, Harvey JW: Serologic diagnosis of infectious cyclic thrombocytopenia in dogs using an indirect fluorescent antibody test. Am J Vet Res 1983, 44:2407-2411
47. Belanger M, Sorenson HL, France MK, Bowie MV, Barbet AF, Breitschwerdt EB, Alleman AR: Comparison of serological detection methods for diagnosis of Ehrlichia canis infections in dogs. J Clin Microbiol 2002, 40:3506-3508
48. Reddy GR, Sulsona CR, Harrison RH, Mahan SM, Burridge MJ, Barbet AF: Sequence heterogeneity of the major antigenic protein 1 genes from Cowdria ruminantium isolates from different geographical areas. Clin Diag Lab Immunol 1996, 3:417-422
49. Uilenberg G, Barre N, Camus E, Burridge MJ, Garris GI: Heartwater in the Caribbean. Prev Vet Med 1984, 2:255-267