Transcriptional inactivation of p53, Bax, Bcl-2 and Mdm2 correlates with malignant transformation of the uterine cervix

International Journal of Biological Markers

Volumn 20, Issue 1, 2005, Pages 18-27

  Soufla, G ^a   Baritaki, S ^a   Sifakis, S ^b   Zafiropoulos, A ^a   Spandidos, D A ^a  

^a UNIVERSITY OF CRETE   (Greece)

^b UNIVERSITY HOSPITAL OF HERAKLION   (Greece)

Author keywords

Apoptosis; Cervical cancer; Cervical intraepithelial neoplasia; mRNA expression; RT PCR

Indexed keywords

MESSENGER RNA; PROTEIN BAX; PROTEIN BCL 2; PROTEIN MDM2; PROTEIN P53;

APOPTOSIS; ARTICLE; CARCINOGENESIS; CELL DEATH; CELL TRANSFORMATION; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION FUNCTION; DEREGULATION; FEMALE; GENETIC TRANSCRIPTION; HUMAN; HUMAN TISSUE; MALIGNANT TRANSFORMATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STATISTICAL SIGNIFICANCE; UTERINE CERVIX; UTERINE CERVIX CANCER; UTERINE CERVIX CARCINOMA IN SITU;

EID: 18744388377     PISSN: 03936155     EISSN: None     Source Type: Journal    
DOI: 10.5301/JBM.2008.4978     Document Type: Article

References (37)

1. Levine AJ, Momand J, Finlay CA. The p53 tumour suppressor gene. Nature 1991; 351: 453-6.
2. Hall PA, Meek D, Lane DP. p53 - integrating the complexity. J Pathol 1996; 180: 1-5.
3. Jin S, Levine AJ. The p53 functional circuit. J Cell Sci 2001; 114: 4139-40.
4. Hickman ES, Moroni MC, Helin K. The role of p53 and pRB in apoptosis and cancer. Curr Opin Genet Dev 2002; 12: 60-6.
5. Hockenbery D, Nunez G, Milliman C, Schreiber RD, Korsmeyer SJ. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348: 334-6.
6. Hockenbery DM. The bcl-2 oncogene and apoptosis. Semin Immunol 1992; 4: 413-20.
7. Kerr JF, Winterford CM, Harmon BV. Apoptosis. Its significance in cancer and cancer therapy. Cancer 1994; 73: 2013-26.
8. Liang XH, Mungal S, Ayscue A, et al. Bcl-2 protooncogene expression in cervical carcinoma cell lines containing inactive p53. J Cell Biochem 1995; 57: 509-21.
9. Saegusa M, Takano Y, Hashimura M, Shoji Y, Okayasu I. The possible role of bcl-2 expression in the progression of tumors of the uterine cervix. Cancer 1995; 76: 2297-303.
10. Ter Harmsel B, Smedts F, Kuijpers J, Jeunink M, Trimbos B, Ramaekers F. BCL-2 immunoreactivity increases with severity of CIN: a study of normal cervical epithelia, CIN, and cervical carcinoma. J Pathol 1996; 179: 26-30.
11. Sheets EE, Yeh J. The role of apoptosis in gynaecological malignancies. Ann Med 1997; 29: 121-6.
12. Hsu SY, Hsueh AJ. Tissue-specific Bcl-2 protein partners in apoptosis: An ovarian paradigm. Physiol Rev 2000; 80: 593-614.
13. Boise LH, Gonzalez-Garcia M, Postema CE, et al. bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell 1993; 74: 597-608.
14. Kernohan NM, Cox LS. Regulation of apoptosis by Bcl-2 and its related proteins: immunochemical challenges and therapeutic implications. J Pathol 1996; 179: 1-3.
15. Wyllie AH. Apoptosis. Death gets a brake. Nature 1994; 369: 272-3.
16. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74: 609-19.
17. Williams GT, Smith CA. Molecular regulation of apoptosis: genetic controls on cell death. Cell 1993; 74: 777-9.
18. Olson DC, Marechal V, Momand J, Chen J, Romocki C, Levine AJ. Identification and characterization of multiple mdm-2 proteins and mdm-2-p53 protein complexes. Oncogene 1993; 8: 2353-60.
19. Cheung TH, Chung TK, Lo KW, et al. Apotosis-related proteins in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix. Gynecol Oncol 2002; 86: 14-8.
20. Hunt CR, Hale RJ, Buckley CH, Hunt J. p53 expression in carcinoma of the cervix. J Clin Pathol 1996; 49: 971-4.
21. Crawford RA, Caldwell C, Iles RK, Lowe D, Shepherd JH, Chard T. Prognostic significance of the bcl-2 apoptotic family of proteins in primary and recurrent cervical cancer. Br J Cancer 1998; 78: 210-4.
22. Uehara T, Kuwashima Y, Izumo T, Kishi K, Shiromizu K, Matsuzawa M. Expression of the proto-oncogene bcl-2 in uterine cervical squamous cell carcinoma: its relationship to clinical outcome. Eur J Gynaecol Oncol 1995; 16: 453-60.
23. Dokianakis DN, Sourvinos G, Sakkas S, Athanasiadou E, Spandidos DA. Detection of HPV and ras gene mutations in cervical smears from female genital lesions. Oncol Rep 1998; 5; 1195-8
24. Sherbet GV, Patil D. Genetic abnormalities of cell proliferation, invasion and metastasis, with special reference to gynaecological cancers. Anticancer Res 2003; 23: 1357-71.
25. Jain D, Srinivasan R, Patel FD, Kumari Gupta S. Evaluation of p53 and Bcl-2 expression as prognostic markers in invasive cervical carcinoma stage IIb/III patients treated by radiotherapy. Gynecol Oncol 2003; 88: 22-8.
26. Giarnieri E, Mancini R, Pisani T, Alderisio M, Vecchione A. Msh2, Mlh1, Fhit, p53, Bcl-2, and Bax expression in invasive and in situ squamous cell carcinoma of the uterine cervix. Clin Cancer Res 2000; 6: 3600-6.
27. Tjalma WA, Weyler JJ, Bogers JJ, et al. The importance of biological factors (bcl-2, bax, p53, PCNA, MI, HPV and angiogenesis) in invasive cervical cancer. Eur J Obstet Gynecol Reprod Biol 2001; 97: 223-30.
28. Huang LW, Chou YY, Chao SL, Chen TJ, Lee TT. p53 and p21 expression in precancerous lesions and carcinomas of the uterine cervix: overexpression of p53 predicts poor disease outcome. Gynecol Oncol 2001; 83: 348-54.
29. Dimitrakakis C, Kymionis G, Diakomanolis E, et al. The possible role of p53 and bcl-2 expression in cervical carcinomas and their premalignant lesions. Gynecol Oncol 2000; 77: 129-36.
30. Kokawa K, Shikone T, Otani T, Nakano R. Apoptosis and the expression of Bax and Bcl-2 in squamous cell carcinoma and adenocarcinoma of the uterine cervix. Cancer 1999; 85: 1799-809.
31. Ferrandina G, Mozzetti S, Marone M, et al. Bcl-2, bax, bcl-x(L) and bcl-x(S) expression in neoplastic and normal cervical tissue. Cancer Lett 2000; 155: 19-27.
32. McCluggage G, McBride H, Maxwell P, Bharucha H. Immunohistochemical detection of p53 and bcl-2 proteins in neoplastic and non-neoplastic endocervical glandular lesions. Int J Gynecol Pathol 1997; 16: 22-7.
33. Nakamura T, Nomura S, Sakai T, Nariya S. Expression of bcl-2 oncoprotein in gastrointestinal and uterine carcinomas and their premalignant lesions. Hum Pathol 1997; 28: 309-15.
34. Iervolino A, Trisciuoglio D, Ribatti D, et al. Bcl-2 overexpression in human melanoma cells increases angiogenesis through VEGF mRNA stabilization and HIF-1-mediated transcriptional activity. Faseb J 2002; 16: 1453-5.
35. Miyashita T, Krajewski S, Krajewska M, et al. Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene 1994; 9: 1799-805.
36. Spandidos DA, Sourvinos G, Tsatsanis C, Zafiropoulos A. Normal ras genes: their onco-suppressor and pro-apoptotic functions (review). Int J Oncol 2002; 21: 237-41.
37. Miyashita T, Reed JC. Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 1995; 80: 293-9.