A new type of dihydroorotate dehydrogenase, type 1S, from the thermoacidophilic archaeon Sulfolobus solfataricus

Extremophiles

Volumn 6, Issue 3, 2002, Pages 245-251

  Sørensen, Palle Gravgaard ^a   Dandanell, Gert ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Characterization; Dihydroorotate dehydrogenase; Purification; Sequence analysis; Sulfolobus solfataricus

Indexed keywords

ARCHAEA; SULFOLOBUS SOLFATARICUS;

DIHYDROOROTATE DEHYDROGENASE; OXIDOREDUCTASE;

ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; KINETICS; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; SULFOLOBUS;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; KINETICS; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; SUBSTRATE SPECIFICITY; SULFOLOBUS;

EID: 18744363004     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-001-0249-0     Document Type: Article

References (15)

1. Argyrou A, Washabaugh MW, Pickart CM (2000) Dihydroorotate dehydrogenase from Clostridium oroticum is a class 1B enzyme and utilizes a concerted mechanism of catalysis. Biochemistry 39:10373-10384
2. Björnberg O, Grüner A-C, Roepstorff P, Jensen KF (1999) The activity of Escherichia coli dihydroorotate dehydrogenase is dependent on a conserved loop identified by sequence homology, mutagenesis, and limited proteolysis. Biochemistry 39:2899-2908
3. Cleland WW (1971) Steady state kinetics. In: Boyer PD (ed) The enzymes, 3rd edn, vol 2. Academic Press, New York, pp 1-65
4. Hines V, Keys LD, Johnston M (1986) Purification and properties of the bovine liver mitochondrial dihydroorotate dehydrogenase. J Biol Chem 261:11386-11392
5. Jensen KF, Björnberg O (1998) Evolutionary and functional families of dihydroorotate dehydrogenases. In: Paths to pyrimidines, vol 6. Dept. of Microbiology & Immunology, University of Kentucky, Lexington, pp 20-28
6. Jordan DB, Bisaha JB, Picollelli MA (2000) Catalytic properties of dihydroorotate dehydrogenase from Saccharomyces cerevisiae: studies on pH, alternate substrates, and inhibitors. Arch Biochem Biophys 378:84-92
7. Karibian D (1978) Dihydroorotate dehydrogenase (Escherichia coli). Methods Enzymol 51:58-63
8. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature. 227:680-685.
9. Liu S, Neidhardt EA, Grossmann TH, Ocain T, Clardy J (2000) Structures of human dihydroorotate dehydrogenase in complex with antiproliferative agents. Structure 8:25-33
10. Miller RW (1978) Dihydroorotate dehydrogenase (Neurospora crassa). Methods Enzymol. 51:63-69
11. Nielsen FS, Andersen PS, Jensen KF (1996a) The B form of dihydroorotate dehydrogenase from Lactococcus lactis consists of two different subunits, encoded by pyrDb and pyrK genes, and contains FMN, FAD, and [FeS] redox centers. J Biol Chem 271:29359-29365
12. Nielsen FS, Rowland P, Larsen S, Jensen KF (1996b) Purification and characterization of dihydroorotate dehydrogenase A from Lactococcus lactis: crystallization and preliminary X-ray diffraction studies of the enzyme. Protein Sci 5:852-856
13. Pascal RA, Walsh CT (1984) Mechanistic studies with deuterated dihydroorotates on the dihydroorotate oxidase from Crithidia fasciculata. Biochemistry 23:2745-2752
14. Rowland P, Nørager S, Jensen KF, Larsen S (2000) Structure of dihydroorotate dehydrogenase B: electron transfer between two flavin groups bridged by an iron-sulphur cluster. Structure 8:1227-1238
15. Sørensen PG, Dandanell G (1999) Dihydroorotate dehydrogenase from the thermoacidophilic archaeon Sulfolobus solfataricus is a cytosolic dimer. In: Ghisla S, Kroneck P, Macheroux P, Sund H (eds) Flavins and flavoproteins. Weber, Berlin, pp 619-622