Cardiac myofibril formation is not affected by modification of Both N- and C-termini of sarcomeric tropomyosin

Cardiovascular Toxicology

Volumn 5, Issue 1, 2005, Pages 1-8

  Narshi, Aruna ^a,b   Denz, Christopher K ^a   Nakatsugawa, Masako ^a   Zajdel, Robert W ^a   Dube, Syamalima ^a   Poiesz, Bernard J ^a   Dube, Dipak K ^a  

^a State University of New York Upstate Medical University: College of Medicine   (United States)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Amphibia; Confocal microscopy; Electron microscopy; FLAG; GFP fusion protein; Lipofection

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; TROPOMYOSIN; TROPOMYOSIN 4ALPHA; UNCLASSIFIED DRUG;

AMBYSTOMA MEXICANUM; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EMBRYO; GENE CONSTRUCT; GENETIC TRANSFECTION; HEART CELL CULTURE; HEART MUSCLE; MUSCLE FIBRIL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SARCOMERE; SCANNING ELECTRON MICROSCOPY; SKELETAL MUSCLE; STRUCTURE ACTIVITY RELATION;

AMBYSTOMA MEXICANUM; AMPHIBIA;

EID: 18544381681     PISSN: 15307905     EISSN: None     Source Type: Journal    
DOI: 10.1385/ct:5:1:001     Document Type: Article

References (23)

1. Lees-Miller, J.P. and Helfman, D.M. (1991). The molecular basis of tropomyosin diversity. Bioessays 13:429-437.
2. Perry, S.V. (2001). Vertebrate tropomyosin: distribution, properties and function. J. Muscle Res. Cell Motil. 22:5-49.
3. Lemanski, L.F. (1973). Morphology of developing heart in cardiac lethal mutant Mexican axolotls, Ambystoma mexicanum. Dev. Biol. 33:312-333.
4. Lemanski, L.F. (1979). Role of tropomyosin in actin filament formation in embryonic salamander heart cells. J. Cell Biol. 82:227-238.
5. Zhang, C., Meng, F., Huang, X.P., Zajdel, R., Lemanski, S.L., Foster, D., et al. (2004). Downregulation of N1 gene expression inhibits the initial heart beating and heart development in axolotls. Tissue Cell 36:71-81.
6. Zajdel, R.W., McLean M.D., Lemanski S.L., Muthuchamy, M., Wieczorek, D.F., Lemanski, L.F., et al. (1998). Ectopic expression of tropomyosin promotes myofibrill-zogenesis in mutant axolotl hearts. Dev. Dyn. 213:412-420.
7. Zajdel, R.W., Sanger, J.M., Denz, C.R., Lee, S., Dube, S., Poiesz, B.J., et al. (2002). A novel striated muscle tropomyosin incorporated into organized myofibrils of cardiomyocytes in cell and organ culture. FEBS Lett. 520:35-39.
8. Zajdel, R.W., Choudhury, A., Dube, S., Mehta, S., and Dube, D.K. (2000). Transfection of anti-sense oligonucleotide into whole hearts using cationic liposomes. Focus (Gibco BRL). 22:28-30.
9. Lemanski, L.F., Nakatsugawa, M., Bhatia, R., Erginel-Unaltuna, N., Spinner, B., and Dube, D.K. (1996). Characterization of an RNA which promotes myofibrillogenesis in embryonic cardiac mutant axolotl hearts. Biochem. Biophys. Res. Commun. 229:974-981.
10. Zhang, C., Dube, D.K., Huang, X., Zajdel, R.W., Bhatia, R., Foster, D., et al. (2003). A point mutation in bioactive RNA results in the failure of mutant heart correction in Mexican axolotls. Anat. Embryol. (Berl.) 206:495-506.
11. Luque, E.A., Spinner, B.J., Dube, S., Dube, D.K., and Lemanski, L.F. (1997). Differential expression of a novel isoform of alpha-tropomyosin in cardiac and skeletal muscle of the Mexican axolotl. Gene 185:175-185.
12. Spinner, B.J., Zajdel, R.W., McLean, M.D., Denz, C.R., Dube, S., Mehta, S., et al. (2002). Characterization of a TM4 type tropomyosin that is essential for myofibrillogenesis and contractile activity in embryonic heart of the Mexican axolotl. J. Cell. Biochem. 85:747-761.
13. Zot, H.G., Guth, K., and Potter, J.D. (1986). Fast skeletal muscle skinned fibers and myofibrils reconstituted with N-terminal fluorescent analogues of troponin C. J. Biol. Chem. 261:15883-15890.
14. Palm, T., Greenfield, N.J., and Hitchock-DeGregori, S.E. (2003). Tropomyosin ends determine the stability and functionality of overlap and troponin T complex. Biophys. J. 84:3181-3189.
15. Metzger, J.M., Michele, D.E., Rust, E.M., Borton, A.R., and Westfall, M.V. (2003). Sarcomeric thin filament regulatory isoforms. J. Biol. Chem. 278:13118-13123.
16. Bordzilovskaya, N.P., Detlaff, T.A., Duhon, S.T., and Malacinski, G.M. (1989). Developmental stage series of axolotl embryos, in Developmental Biology of the Axolotl (Armstrong, J.B. and Malacinski, G.M., eds.), Oxford University Press, Oxford, pp. 201-219.
17. Lin, J.J-C., Helfman, D.M., Hughes, S.H., and Chou, C-S. (1985). Tropomyosin isoforms in chicken embryo fibroblasts. Purfication, characterization, and changes in Rous sarcoma virus-transformed cells. J. Cell Biol. 100:692-703.
18. Gimona, M., Watakabe, A., and Helfman, D.M. (1995). Specificity of dimer formation in tropomyosins: influence of alternatively spliced exons on homodimer and heterodimer assembly. Proc. Natl. Sci. Acad. Sci. USA 92:9776-9780.
19. Muthuchamy, M., Pieples, K., Rethinasamy, P., Hoit, B., Grupp, I.L., Boivin, G.P., et al. (1999). Mouse model of a familial hypertrophie cardiomyopathy mutation in α-tropomyosin manifests cardiac dysfunction. Circ. Res. 85:47-56.
20. Bottinelli, R., Coviello, D.A., Redwood, C.S., Pellegrino, M.A., Maron, B.J., Spirito, P., et al. (1997). A mutant tropomyosin that causes hypertrophic cardiomyopathy is expressed in vivo and associated with an increased calcium sensitivity. Circ. Res. 82:106-115.
21. Martson, S.B. and Redwood, C.S. (2003). Modulation of thin filament activation by breakdown or isoform switiching of thin filament proteins physiological and pathological implications. Circ. Res. 93:1170-1178.
22. Maron, B.J., Shirani, J., Poliac, L.C., Mathenge, R., Roberts, W.C., and Mueller, P.O. (1996). Sudden death in young competitive athletes. Clinical, demographic, and pathological profiles. JAMA 276:199-204.
23. McLachlan, A.D. and Stewart, M. (1975). Tropomyosin coiled-coil interactions: evidence for an unstaggered structure. J. Mol. Biol. 98:293-304.