Overcoming backpressure problems during solid-phase synthesis of oligonucleotides

Organic Process Research and Development

Volumn 8, Issue 2, 2004, Pages 271-274

  Moore, Max N ^a   Andrade, Mark ^a   Scozzari, Anthony N ^a   Krotz, Achim H ^a  

^a ISIS PHARMACEUTICALS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID; GLASS; MICROSPHERE; OLIGONUCLEOTIDE; POLYMER; POLYSTYRENE; SILICON DIOXIDE; SOLVENT;

ARTICLE; AUTOMATION; DEPROTECTION REACTION; DRUG SYNTHESIS; EXPOSURE; FLOW RATE; PACKED BED REACTOR; PRESSURE; REACTION ANALYSIS; REDUCTION; SOLID; TECHNIQUE;

EID: 1842558908     PISSN: 10836160     EISSN: None     Source Type: Journal    
DOI: 10.1021/op030213j     Document Type: Article

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11. On 1 mmol scale, the initial weight of support packed in the column is 5 g (0.2 mmol/g nucleoside loading). After 19 synthesis cycles, the weight of the support typically increases by ca. 8 g.
12. In LC columns the backpressure is inversely proportional to the square of the particle diameter.
13. Moore, M., Krotz, A., Andrade, M., Scozzari, A. N., Eds. Synthesis of Oligonucleotides on Solid Support. PCT Int. Appl. WO 0308550, 2003.
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15. Cheruvallath, Z. S.; Carty, R. L.; Moore, M. N.; Capaldi, D. C.; Krotz, A. H.; Wheeler, P. D.; Turney, B. J.; Craig, S. R.; Gaus, H. J.; Scozzari, A. N.; Cole, D. L.; Ravikumar, V. T. Org. Process Res. Dev. 2000, 4, 199-204.