Amperometric Analysis of the Effect of Heavy Metals on the Activity of Isocitric Dehydrogenase

Analytical Letters

Volumn 37, Issue 3, 2004, Pages 415-433

  Rodriguez, Belen Bello ^a   Bolbot, John A ^a   Tothill, Ibtisam E ^a  

^a CRANFIELD UNIVERSITY   (United Kingdom)

Author keywords

Isocitrate dehydrogenase; Metal ion inhibition activation; NADPH; Soil analysis

Indexed keywords

CADMIUM; CALCIUM; COPPER; HEAVY METAL; ISOCITRATE DEHYDROGENASE; LEAD; MERCURY; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SILVER; SILVER CHLORIDE; ZINC;

AMPEROMETRY; ARTICLE; CONCENTRATION (PARAMETERS); ELECTRIC POTENTIAL; ELECTROCHEMISTRY; ELECTRODE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; SCREENING TEST; SOIL ANALYSIS; SOIL POLLUTION; SYNTHESIS; WATER ANALYSIS; WATER POLLUTION;

EID: 1842538851     PISSN: 00032719     EISSN: None     Source Type: Journal    
DOI: 10.1081/AL-120028616     Document Type: Article

References (23)

1. Krawczyk, T.K. Analytical applications of inhibition of enzymatic reactions. Chem. Anal. 1998, 43, 135-158.
2. Shekhovtsova, T.N.; Chernetskaya, S.V.; Belkova, N.V.; Dolmanova, I.F. The use of immobilised enzymes for the determination of metal ions. J. Anal. Chem. 1994, 49, 709-714.
3. Fatania, H.R.; Al-Nassar, K.E.; Thomas, N. Chemical modification of rat liver cytosolic NADP(+)-linked isocitrate dehydrogenase by N-ethylmaleimide. Evidence for essential sulphydryl groups. FEBS Lett. 1993, 322, 245-248.
4. Smyth, G.E.; Colman, R.F. Cysteinyl peptides of pig heart NADP-dependent isocitrate dehydrogenase that are modified upon inactivation by N-ethylmaleimide. J. Biol. Chem. 1991, 266, 14918-14925.
5. Kratochvil, B.; Boyer, S.L.; Hicks, G.P. Effect of metals on the activation and inhibition of isocitric dehydrogenase. Application to trace metals analysis. Anal. Chem. 1967, 39 (1), 45-51.
6. Sheikh, R.A.; Townshend, A. Applications of enzyme-catalysed reactions in trace analysis-VII. Determination of lead and indium by their inhibition of isocitric dehydrogenase. Talanta 1974, 21, 401-409.
7. Islam, M.; Bell, J.L.; Baron, D.N. Purification and comparative properties of isoenzymes of nicotinamide-adenine dinucleotide phosphate-isocitrate dehydrogenase from rat heart and liver. Biochem. J. 1972, 129, 1003-1011.
8. 2+ and ADP on the activity of NAD-linked isocitrate dehydrogenase of muscle. FEBS letters 1969, 5 (2), 124-126.
9. 2S; Editorial Paraninfo: Madrid, 1993.
10. Ellis, G.; Goldberg, D.M. An improved manual and semi-automatic assay for NADP-dependent isocitrate dehydrogenase activity, with a description of some kinetic properties of human liver and serum enzyme. Clin. Biochem. 1971, 2, 175-185.
11. Arif, M.; Setford, S.J.; Burton, K.S.; Tothill, I.E. L-Malic acid biosensor for field-based evaluation of apple, potato and tomato horticultural produce. Analyst 2002, 127, 104-108.
12. Newman, J.D.; White, S.F.; Tothill, I.E.; Turner, A.P.F. Catalytic materials, membranes, and fabrication technologies suitable for the construction of amperometric biosensors. Anal. Chem. 1995, 67, 4594-4599.
13. Kröger, S.; Turner, A.P.F. Solvent-resistant carbon electrodes screen printed onto plastic for use in biosensors. Anal. Chim. Acta. 1997, 347, 9-18.
14. Bello Rodriguez, B.; Bolbot, J.A.; Tothill, I.E. Development of urease and isocitric dehydrogenase amperometric assay for heavy metals screening in polluted samples. Biosens. Bioelectron. 2003, in press.
15. Rodriguez-Granda, P.; Lobo-Castanon, M.J.; Miranda-Ordieres, A.J.; Tuton-Blanco, P. Modified carbon paste electrodes for flow injection amperometric determination of isocitrate dehydrogenase activity in serum. Anal. Biochem. 2002, 308, 195-203.
16. 2-sensing electrodes and its application to isocitrate dehydrogenase activity assay in serum. J. Electroanal. Chem. 1999, 478, 33-39.
17. Andres, R.T.; Narayanaswamy, R. Effect of the coupling reagent on the metal inhibition of immobilised urease in an optical biosensor. Analyst 1995, 120, 1549-1554.
18. Senillou, A.; Jaffrezic-Renault, N.; Martelet, C.; Cosnier, S. A miniaturized urea sensor based on the integration of both ammonium based urea enzyme field effect transistor and a reference field effect transistor in a single chip. Talanta 1999, 50, 219-226.
19. Krajewska, B. Urease immobilized on chitosan membrane. Inactivation by heavy metal ions. J. Chem. Tech. Biotechnol. 1991, 52, 157-162.
20. Alston, T.; Porter, D.J.T.; Bright, H.J. Enzyme inhibition by nitro and nitroso compounds. Acc. Chem. Res. 1983, 16, 418-424.
21. +-dependent isocitrate dehydrogenase by nitric oxide. Free Rad. Biol. Med. 2002, 33 (7), 927-937.
22. +-dependent isocitrate dehydrogenase by reactive oxygen species. Biochimie 2001, 83, 1057-1065.
23. Baum, P.; Czok, R. Enzymatische bestimmung von "ionisierten" magnesium im plasma. Biochem. 1959, 332, 121-130.