Comparative study of monocyte enumeration by flow cytometry: Improved detection by combining monocyte-related antibodies with anti-CD163

Laboratory Hematology

Volumn 10, Issue 1, 2004, Pages 24-31

  Zarev, Petar V ^a   Davis, Bruce H ^a  

^a Maine Medical Center Research Institute   (United States)

Author keywords

CD163; Cell counting; Hematology analyzers; Monocyte antigens; Reference methods

Indexed keywords

CELL ANTIBODY; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY CD116; MONOCLONAL ANTIBODY CD14; MONOCLONAL ANTIBODY CD145; MONOCLONAL ANTIBODY CD163; MONOCLONAL ANTIBODY CD64; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BLOOD SAMPLING; CELL COUNT; CELL LABELING; COLOR; CONTROLLED STUDY; EVALUATION; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; MONOCYTE; PROCESS OPTIMIZATION;

ANTIBODIES, MONOCLONAL; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; FEMALE; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; LEUKOCYTE COUNT; MALE; MONOCYTES; RECEPTORS, CELL SURFACE; SENSITIVITY AND SPECIFICITY;

EID: 1842431937     PISSN: 10802924     EISSN: None     Source Type: Journal    
DOI: 10.1532/LH96.03056     Document Type: Article

References (29)

1. Goossens W, Van Hove L, Verwilghen RL. Monocyte counting: discrepancies in results obtained with different automated instruments. J Clin Pathol. 1991;44:224-227.
2. Hubl W, Hauptlorenz S, Tlustos L, et al. Precision and accuracy of monocyte counting: Comparison of two hematology analyzers, the manual differential and flow cytometry. Am J Clin Pathol. 1995; 103:167-170.
3. Morillon M, Maslin J, De Pina JJ, et al. Evaluation of the monocyte counting by the ABX Vega: Comparison with the manual method and fluoro-flow cytometry. Hematol Cell Ther. 1999;41: 47-50.
4. NCCLS. Reference Leukocyte Differential Count (Proportional) and Evaluation of Instrumental Methods: Approved Standard. Villanova, Pa: NCCLS; 1992. NCCLS Document H-20A.
5. Koepke JA, Dotson MA, Shifman MA. A critical evaluation of the manual/visual differential leukocyte counting method. Blood Cells. 1985;11:173-186.
6. Hubl W, Andert S, Erath A, et al. Peripheral blood monocyte counting: Towards a new reference method. Eur J Clin Chem Clin Biochem. 1995;33:839-845.
7. Hubl W, Tlustos L, Erath A, et al. Proposed reference method for peripheral-blood monocyte counting using fluorescence-labelled monoclonal antibodies. Cytometry. 1996;26:69-74.
8. Hubl W, Tlustos L, Bayer PM. Use of precision profiles to evaluate precision of the automated leukocyte differential. Clin Chem. 1996;42:1068-1073.
9. Hubl W, Wolfbauer G, Andert S, et al. Toward a new reference method for the leukocyte five-part differential. Cytometry. 1997; 30:72-84.
10. Grage-Griebenow E, Flad HD, Ernst M. Heterogeneity of human peripheral blood monocyte subsets. J Leukoc Biol, 2001;69:11-20.
11. Szeberenyi JB, Rothe G, Pallinger E, et al. Multi-color analysis of monocyte and dendritic cell precursor heterogeneity in whole blood. Immunobiology. 2000;202:51-58.
12. Ziegler-Heitbrock HW. Definition of human blood monocytes. J Leukoc Biol. 2000;67:603-606.
13. Zwadlo-Klarwasser G, Vogts M, Hamann W, et al. Generation and subcellular distribution of histamine in human blood monocytes and monocyte subsets. Inflamm Res. 1998;47:434-439.
14. Graversen JH, Madsen M, Moestrup SK. CD163: A signal receptor scavenging haptoglobin-hemoglobin complexes from plasma. Int J Biochem Cell Biol. 2002;34:309-314.
15. Madsen M, Graversen JH, Moestrup SK. Haptoglobin and CD163: captor and receptor gating hemoglobin to macrophage lysosomes. Redox Rep. 2001;6:386-388.
16. Sulahian TH, Hogger P, Wahner AE, et al. Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. 2000;12:1312-1321.
17. Högger P, Dreier J, Droste A, et al. Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163). J Immunol. 1998;161:1883-1890.
18. Kristiansen M, Graversen JH, Jacobsen C, et al. Identification of the haemoglobin scavenger receptor. Nature. 2001;409:198-201.
19. Hintz KA, Rassias AJ, Wardwell K, et al. Endotoxin induces rapid metalloproteinase-mediated shedding followed by up-regulation of the monocyte hemoglobin scavenger receptor CD163. J Leukoc Biol. 2002;72:711-717.
20. Matsushita N, Kashiwagi M, Wait R, et al. Elevated levels of soluble CD163 in sera and fluids from rheumatoid arthritis patients and inhibition of the shedding of CD163 by TIMP-3. Clin Exp Immunol. 2002;130:156-161.
21. Buechler C, Ritter M, Orso E, et al. Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuli. J Leukoc Biol. 2000;67:97-103.
22. Knapp W, Dorken K, Gilks WR, et al, eds. Leukocyte Typing IV: White Cell Differentiation Antigens. New York: Oxford University Press; 1989.
23. Wright SD, Ramos RA, Tobias PS, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990;249:1431-1433.
24. Wagner C, Deppisch R, Denefleh B, et al. Expression patterns of the lipopolysaccharide receptor CD14, and the FCgamma receptors CD16 and CD64 on polymorphonuclear neutrophils: Data from patients with severe bacterial infections and lipopolysaccharide-exposed cells. Shock. 2003;19:5-12.
25. Hubl W, Andert S, Thum G, et al. Value of neutrophil CD16 expression for detection of left shift and acute-phase response. Am J Clin Pathol. 1997;107:187-196.
26. Januszkiewicz A, Lore K, Essen P, et al. Response of in vivo protein synthesis in T lymphocytes and leucocytes to an endotoxin challenge in healthy volunteers. Clin Exp Immunol. 2002;130: 263-270.
27. Gluck T, Silver J, Epstein M, et al. Parameters influencing membrane CD14 expression and soluble CD14 levels in sepsis. Eur J Med Res. 2001;6:351-358.
28. + subset and soluble CD14 as biological markers of inflammatory systemic diseases and monitoring immunosuppressive therapy. Clin Chem Lab Med. 1999;37:209-213.
29. Barth E, Fischer G, Schneider EM, et al. Differences in the expression of CD64 and mCD14 on polymorphonuclear cells and on monocytes in patients with septic shock. Cytokine. 2001;14:299-302.