Response of in vivo protein synthesis in T lymphocytes and leucocytes to an endotoxin challenge in healthy volunteers

Clinical and Experimental Immunology

Volumn 130, Issue 2, 2002, Pages 263-270

  Januszkiewicz, A ^a   Lore K ^a   Essen P ^a   Andersson, B ^b   Mcnurlan, M A ^c   Garlick, P J ^c   Ringden O ^a   Andersson, J ^a   Wernerman, J ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b Nova Medical Laboratories   (Sweden)

^c STONY BROOK UNIVERSITY   (United States)

Author keywords

Cell subpopulations; Cytokines; Flow cytometry; L 2H5 phenylalanine; Mass spectrometry

Indexed keywords

CD15 ANTIGEN; CD3 ANTIGEN; CD56 ANTIGEN; ENDOTOXIN; INTERLEUKIN 1 RECEPTOR BLOCKING AGENT; INTERLEUKIN 10; INTERLEUKIN 6; INTERLEUKIN 8; SODIUM CHLORIDE; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BLOOD CELL COUNT; BLOOD LEVEL; CELL POPULATION; CELL SUBPOPULATION; CONTROLLED STUDY; FLOW CYTOMETRY; GRANULOCYTE; HUMAN; IMMUNOCOMPETENT CELL; IN VIVO STUDY; LEUKOCYTE; MALE; METABOLIC ACTIVATION; MONOCYTE; NATURAL KILLER CELL; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; T LYMPHOCYTE;

ADULT; ANTIGENS, CD15; ANTIGENS, CD3; ANTIGENS, CD56; CYTOKINES; ENDOTOXINS; HUMANS; KILLER CELLS, NATURAL; LEUKOCYTE COUNT; LEUKOCYTES; MALE; NEUTROPHILS; PROTEIN BIOSYNTHESIS; SEPSIS; T-LYMPHOCYTES;

EID: 0036405039     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2249.2002.01983.x     Document Type: Article

References (34)

1. Hardaway RM. A review of septic shock. Am Surg 2000; 66:22-9.
2. Pittet D, Harbarth S, Suter PM et al. Impact of immunomodulating therapy on morbidity in patients with severe sepsis. Am J Respir Crit Care Med 1999; 160:852-7.
3. Velmahos GC, Demetriades D, Shoemaker WC et al. Endpoints of resuscitation of critically injured patients: Normal or supranormal? A prospective randomized trial. Ann Surg 2000; 232:409-18.
4. Bellingan G. Inflammatory cell activation in sepsis. Br Med Bull 1999; 55:12-29.
5. Volk HD, Reinke P, Döcke WD. Clinical aspects: From systemic inflammation to immunoparalysis. Chem Immunol 2000; 74:162-77.
6. Keh D. Immunocompetence in the critically ill: Understanding the problem. Minerva Anestesiol 2000; 66:358-61.
7. Cheadle WG, Pemberton RM, Robinson D, Livingston DH, Rodriguez JL, Polk HC. Lymphocyte subset responses to trauma and sepsis. J Trauma 1993; 35:844-9.
8. Heidecke CD, Hensler T, Weighardt H et al. Selective defects of T lymphocyte function in patients with lethal intraabdominal infection. Am J Surg 1999; 178:288-92.
9. Smith CJ, Edwards AE, Gemmel LW, Ferguson BJ, Gower DE, Gough JD. Lectin-stimulated lymphocyte responses: Use and misuse in clinical research. Eur J Anaesthesiol 1988; 5:269-78.
10. Bellomo R. The cytokine network in the critically ill. Anaesth Intensive Care 1992; 20:288-302.
11. Casey LC. Immunologic response to infection and its role in septic shock. Crit Care Clin 2000; 16:193-213.
12. Volk HD, Reinke P, Döcke WD. Immunological monitoring of the inflammatory process: Which variables? When to assess? Eur J Surg (Suppl) 1999; 584:70-2.
13. Ditschkowski M, Kreuzfelder E, Rebmann V et al. HLA-DR expression and soluble HLA-DR levels in septic patients after trauma. Ann Surg 1999; 229:246-54.
14. Januszkiewicz A, Essen P, McNurlan MA, Ringden O, Garlick PJ, Wernerman J. A combined stress hormone infusion decreases in vivo protein synthesis in human T lymphocytes in healthy volunteers. Metabolism 2001; 11:1308-14.
15. Januszkiewicz A, Essen P, McNurlan MA, Ringden O, Wernerman J, Garlick PJ.In vivo protein synthesis of circulating human T lymphocytes does not respond to a cortisol challenge within 24 hours. Acta Anaesthesiol Scand 2000; 44:202-9.
16. Essen P, McNurlan MA, Thorell A et al. Determination of protein synthesis in lymphocytes in vivo after surgery. Clin Sci 1996; 91:99-106.
17. Danner RL, Elin RJ, Hosseini JM, Wesley RA, Reilly JM, Parillo JE. Endotoxemia in human septic shock. Chest 1991; 99:169-75.
18. Lin E, Lowry SF. The human response to endotoxin. Sepsis 1998; 2:255-62.
19. Bøyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol 1976; 5 (Suppl. 5):9-15.
20. Parker JW. Immunologic basis for the redefinition of malignant lymphomas. Am J Clin Pathol 1979; 72:670-86.
21. 5]phenylalanine. Am J Physiol 1994; 267:E102-8.
22. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev 1991; 119:65-93.
23. Lore K, Sonnerborg A, Spetz AL, Andersson U, Andersson J. Erratum to 'Immunocytochemical detection of cytokines and chemokines in Langerhans cells and in vitro derived dendritic cells'. J Immunol Methods 1998; 218:173-87.
24. Martich GD, Boujoukos AJ, Suffredini AF. Response of man to endotoxin. Immunobiol 1993; 187:403-16.
25. de Vries JE. Immunosuppressive and anti-inflammatory properties of interleukin 10. Ann Med 1995; 27:537-41.
26. Zimmer S, Pollard V, Marshall GD, Garofalo RP, Traber D, Prough D, Herndon DN. The 1996 Moyer award. Effects of endotoxin on the Th1/Th2 response in humans. J Burn Care Rehabil 1996; 17:491-6.
27. Lauw FN, ten Hove T, Dekkers PEP, de Jonge E, van Deventer SJH, van der Poll T. Reduced Th1, but not Th2, cytokine production by lymphocytes after in vivo exposure of healthy subjects to endotoxin. Infect Immun 2000; 68:1014-8.
28. Richardson RP, Rhyne CD, Fong Y et al. Peripheral blood leukocyte kinetics following in vivo lipopolysaccharide (LPS) administration to normal human subjects. Ann Surg 1989; 210:239-45.
29. Fletcher J, Haynes AP, Crouch SM. Acquired abnormalities of polymorphonuclear neutrophil function. Blood Rev 1990; 4:103-10.
30. Gosling P. The cellular immune and metabolic response to trauma. Crit Rev Clin Lab Sci 1998; 35:59-112.
31. Cassatella MA. The production of cytokines by polymorphonuclear neutrophils. Immunol Today 1995; 16:21-6.
32. Schultz MJ, Olszyna DP, de Jonge E, Verbon A, van Deventer SJH, van der Poll T. Reduced ex vivo chemokine production by polymorphonuclear cells after in vivo exposure of normal humans to endotoxin. J Infect Dis 2000; 182:1264-7.
33. Kim PK, Deutschman CS. Inflammatory responses and mediators. Surg Clin North Am 2000; 80:885-94.
34. Granowitz EV, Porat R, Mier JW et al. Intravenous endotoxin suppresses the cytokine response of peripheral blood mononuclear cells of healthy humans. J Immunol 1993; 151:1637-45.