Substitution of arginine for cysteine 643 of the glucocorticoid receptor reduces its steroid-binding affinity and transcriptional activity

Cancer Letters

Volumn 181, Issue 1, 2002, Pages 109-114

  Nagano, Michiyo ^a   Nakamura, Takahiro ^a   Niimi, Shingo ^a   Fujino, Tomofumi ^a   Nishimura, Tetsuji ^a   Murayama, Norie ^a   Ishida, Seiichi ^a   Ozawa, Shogo ^a   Saito, Yoshiro ^a   Sawada, Jun Ichi ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

Dexamethasone; Glucocorticoid receptor; Glucocorticoids; Leukemic cells; Transcription

Indexed keywords

ARGININE; CYSTEINE; DEXAMETHASONE; GLUCOCORTICOID RECEPTOR;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CLINICAL ARTICLE; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME ASSAY; EXON; GENE MUTATION; HETEROZYGOSITY; HUMAN; HUMAN CELL; LEUKEMIA CELL; LIGAND BINDING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; STEROID BINDING; WESTERN BLOTTING;

AMINO ACID SUBSTITUTION; ARGININE; CYSTEINE; DEXAMETHASONE; DNA MUTATIONAL ANALYSIS; HUMANS; LEUKEMIA; PROTEIN BINDING; RECEPTORS, GLUCOCORTICOID; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED;

EID: 18344393604     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3835(02)00042-3     Document Type: Article

References (20)

1. Steroid receptor interactions with heat shock protein and immunophilin chaperones
2. Mechanisms of glucocorticoid-receptor-mediated repression of gene expression
3. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
4. Glucocorticoid-induced apoptosis of lymphoid cells
5. Childhood leukemias
6. Glucocorticoid resistance in childhood leukemia
7. Potential limitation of growth-inhibitory action of recombinant human tumor necrosis factor (PAC-4D) due to easy induction of resistance: Evidence in vitro
8. Splice site mutation in the glucocorticoid receptor gene causes resistance to glucocorticoid-induced apoptosis in a human acute leukemic cell line
9. Glucocorticoid receptor gene mutations in leukemic cells acquired in vitro and in vivo
10. Resistance to glucocorticoid-induced apoptosis in human T-cell acute lymphoblastic leukemia CEM-C1 cells is due to insufficient glucocorticoid receptor expression
11. Apparent low frequency of sequence variability within the proximal promoter region of the cytochrome P450 (CYP) 3A5 gene in established cell lines from Japanese individuals
12. Functional domains of the human glucocorticoid receptor
13. Regulation of glucocorticoid receptor by the tyrosine kinase inhibitor herbimycin A in the cytosolic fraction of primary cultured rat hepatocytes
14. Effect of dexamethasone pretreatment on the dexamethasone-dependent induction of tyrosine aminotransferase activity in primary cultured rat hepatocytes
15. A novel pre-T cell line derived from acute lymphoblastic leukemia
16. Role of cysteines 640, 656, and 661 in steroid binding to rat glucocorticoid receptors
17. The aggregation of proteins for small molecules, ions
18. Trans-retinoic acid and glucocorticoids synergistically induce transcription from the mouse mammary tumor virus promoter in human embryonic kidney cells
19. Glucocorticoid receptor thiols and steroid-binding activity
20. Cysteines 638 and 665 in the hormone binding domain of human glucocorticoid receptor define the specificity to glucocorticoids