Combined transcriptome and genome analysis of single micrometastatic cells

Nature Biotechnology

Volumn 20, Issue 4, 2002, Pages 387-392

  Klein, Christoph A ^a   Seidl, Stefan ^a   Petat Dutter, Karina ^a   Offner, Sonja ^c   Geigl, Jochen B ^a   Schmidt Kittler, Oleg ^a   Wendler, Nicole ^a   Passlick, Bernward ^a   Huber, Rudolf M ^a   Schlimok, Günter ^b   Baeuerle, Patrick A ^c   Riethmüller, Gert ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b II Medizinische Klinik Klinikum   (Germany)

^c MICROMET AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DISEASE CONTROL; DRUG THERAPY; ENZYMES; TUMORS;

MICROMETASTATIC CELLS;

GENES;

CD147 ANTIGEN; CYTOKERATIN; ENZYME ACTIVATOR; MATRIX METALLOPROTEINASE; UNCLASSIFIED DRUG;

ADJUVANT THERAPY; ARTICLE; BONE MARROW; CANCER SURGERY; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; GENETIC ANALYSIS; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MAJOR CLINICAL STUDY; METASTASIS; MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TUMOR CELL;

CELL CYCLE; GENE EXPRESSION PROFILING; GENOME, HUMAN; GENOMICS; HUMANS; NEOPLASM METASTASIS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RNA, MESSENGER; RNA, NEOPLASM; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED;

EID: 18344382135     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0402-387     Document Type: Article

References (40)

1. Micrometastatic cancer cells in bone marrow: In vitro detection with anti-cytokeratin and in vivo labeling with anti-17-1A monoclonal antibodies
2. Detection and clinical importance of micrometastatic disease
3. Prognostic significance of micrometastatic tumour cells in bone marrow of colorectal cancer patients
4. Frequency and prognostic significance of isolated tumor cells in bone marrow of patients with non-small-cell lung cancer without overt metastases
5. Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II or III breast cancer
6. The biology and analysis of single disseminated tumour cells
7. Gene expression profiles of laser-captured adjacent neuronal subtypes
8. The epithelial cell surface antigen 17-1A, a target for antibody-mediated tumor therapy: Its biochemical nature, tissue distribution and recognition by different monoclonal antibodies
9. Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells
10. PCR-based cDNA library construction: General cDNA libraries at the level of a few cells
11. Construction of cDNA libraries from single cells
12. In situ isolation of mRNA from individual plant cells: Creation of cell-specific cDNA libraries
13. Gene expression in individual cells: Analysis using global single cell reverse transcription polymerase chain reaction (GSC RT-PCR)
14. A high-density probe array sample preparation method using 10- to 100-fold fewer cells
15. Loss of genomic methylation causes p53-dependent apoptosis and epigenetic deregulation
16. Quantitative evaluation of the expression of MAGE genes in tumors by limiting dilution of cDNA libraries
17. Heterogeneous expression of MAGE-A genes in occult disseminated tumor cells: A novel multimarker reverse transcription-polymerase chain reaction for diagnosis of micrometastatic disease
18. Establishment of a human cell line (Mono Mac 6) with characteristics of mature monocytes
19. NF-κB ten years after
20. Tolerance to lipopolysaccharide involves mobilization of nuclear factor κB with predominance of p 50 homodimers
21. Prothymosin-α is a chromatin-remodelling protein in mammalian cells
22. A human peptidyl-prolyl isomerase essential for regulation of mitosis
23. A cytokinesis checkpoint requiring the yeast homologue of an APC-binding protein
24. Cancer cell cycles
25. The urokinase-type plasminogen activator system in cancer metastasis: A review
26. Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase
27. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
28. Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton
29. Mutations in the DNA ligase I gene of an individual with immunodeficiencies and cellular hypersensitivity to DNA-damaging agents
30. Evidence for DNA-PK-dependent and -independent DNA double-strand break repair pathways in mammalian cells as a function of the cell cycle
31. Quality control by DNA repair
32. Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells
33. Control of apoptosis and mitotic spindle checkpoint by survivin
34. T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density
35. The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobulin superfamily
36. Tumor collagenase stimulatory factor (TCSF) expression and localization in human lung and breast cancers
37. ErbB2 overexpression on occult metastatic cells in bone marrow predicts poor clinical outcome of stage I-III breast cancer patients
38. Addition of homopolymers to the 3′-ends of duplex DNA with terminal transferase