Development of a method for the concentration and recovery of microsporidia from tap water

Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

Volumn 40, Issue 5, 2005, Pages 913-925

  Stine, Scott W ^a   Vladich, Frank D ^b   Pepper, Ian L ^a   Gerba, Charles P ^a  

^a UNIVERSITY OF ARIZONA   (United States)

^b UNIVERSITY OF ARIZONA   (United States)

Author keywords

Concentration; Encephalitozoon cuniculi; Encephalitozoon intestinalis; Microsporidia; Tap water

Indexed keywords

CENTRIFUGATION; DETERGENTS; FILTRATION; PURIFICATION;

INTRACELLULAR PARASITES; MICROSPORIDIAN SPORES; STAINING METHOD; TAP WATER;

WATER;

BUFFER; CALCIUM FLUORIDE; DETERGENT; TAP WATER;

ARTICLE; CONCENTRATION (PARAMETERS); DEVICE; DISEASE TRANSMISSION; ELUTION; ENCEPHALITOZOON CUNICULI; ENCEPHALITOZOON INTESTINALIS; FILTER; FILTRATION; METHODOLOGY; MICROSPORIDA; NONHUMAN; PARAMETER; PURIFICATION; SUCROSE DENSITY GRADIENT CENTRIFUGATION; WATER ANALYSIS; WATER CONTAMINATION; WATER SAMPLING;

ANIMALS; CENTRIFUGATION; ENCEPHALITOZOON; FILTRATION; SPECIMEN HANDLING; SPORES, PROTOZOAN; WATER; WATER SUPPLY;

ENCEPHALITOZOON CUNICULI; ENCEPHALITOZOON INTESTINALIS; MICROSPORIDIA;

EID: 18244366855     PISSN: 10934529     EISSN: None     Source Type: Journal    
DOI: 10.1081/ESE-200056105     Document Type: Article

References (17)

1. Matsubayashi, H.K.; Mikata, T.; Hagiwara, S. A case of Encephalitozoon-like infection in man. Arch. Pathol. 1959, 67, 181-187.
2. Franzen, C.; Miller, A. Molecular techniques for detection, species differentiation, and phylogenetic analysis of microsporidia. Clin. Microbiol. Rev. 1999, 12, 243-285.
3. Bryan, R.T.; Weber, R.; Schwartz, D.A. Microsporidiosis in patients who are not infected with human immunodeficiency virus. Clin. Infect. Dis. 1997, 24, 534-535.
4. Weber, R.; Bryan, R.T. Microsporidial infections in immunodeficient and immunocompetent patients. Clin. Infect. Dis. 1994, 19, 517-521.
5. Cotte, L.; Rabodonirina, M.; Chapuis, F.; Bailly, F.; Bissuel, F.; Raynal, C.; Gelas, P.; Persat, F.; Piens, M.A.; Trepo, C. Waterborne outbreak of intestinal microsporidiosis in persons with and without human immunodeficiency virus. J. Infect. Dis. 1999, 180, 2003-2008.
6. Dowd, S.E.; Gerba, C.P.; Pepper, I.L. Confirmation of the human-pathogenic microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vitta corneae in water. Appl. Environ. Microbiol. 1998, 64, 3332-3335.
7. Sorel, N.; Guillot, E.; Thellier, M.; Accoceberry, I.; Datry, A.; Mesnard-Rouiller, L.; Miegeville, M. Development of an immunomagnetic separation-polymerase chain reaction (IMS-PCR) assay specific for Enterocytozoon bieneusi in water samples. J. Appl. Microbiol. 2003, 94, 273-279.
8. John, D.E.; Nwachuku, N.; Pepper, I.L.; Gerba, C.P. Development and optimization of a quantitative cell culture infectivity assay for the microsporidium Encephalitozoon intestinalis and application to ultraviolet light inactivation. J. Microbiol. Methods 2003, 52, 183-196.
9. United States Environmental Protection Agency (USEPA). Method 1622: Cryptosporidium in Water by Filtration / IMS / FA. EPA 821-R99-006; U.S. Environmental Protection Agency: Washington, DC, 1999.
10. Panelli, M. Concentration and detection of Septata intestinalis in water. Unpublished thesis, University of Arizona, Tucson, AZ, 1996.
11. Kamper, M.F. The occurrence of microsporidia in environmental waters. Unpublished thesis, University of Arizona, Tucson, AZ, 1997.
12. www.pall.com/gelman/micro_an/papers/envirochek.asp (accessed June 2001).
13. Dufour, A.F.; Lindquist, A.; Messener, M.; Regli, S.; Rodgers, C.; Schaefer, F.; Schaub, S.; Sinclair, J.; Wymer, L. Criteria for Evaluation of Proposed Protozoan Detection Methods. U.S. Environmental Protection Agency: Washington, DC, 1999.
14. Li, X.; Tate, K.W.; Dunbar, L.A.; Huang, B.; Atwill, E.R. Efficiency for recovering Encephalitozoon intestinalis spores from waters by centrifugation and immunofluorescence microscopy. J. Eukaryot. Microbiol. 2003, 50, 579-580.
15. Borchardt, M.A.; Spencer, S.K. Concentration of Cryptosporidium, microsporidia and other water-borne pathogens by continuous separation channel centrifugation. Appl. Environ. Microbiol. 2002, 92, 649-656.
16. Matheson, Z.; Hargey, T.M.; McLuin, R.M.; Clancy, J.L.; Fricker, C.R. An evaluation of the Gelman Envirochek capsule for the simultaneous concentration of Cyrptosporidium and Giardia from water. J. Appl. Microbiol. 1998, 85, 755-761.
17. John, D.E. Personal communication, 2000.