Use of a simple semiquantitative method for appraisal of green fluorescent protein gene expression in transgenic tobacco plants

Biologia Plantarum

Volumn 49, Issue 2, 2005, Pages 313-316

  Hraska M ^b   Rakousky S ^b   Kocabek T ^a  

^a INSTITUTE OF PLANT MOLECULAR BIOLOGY   (Czech Republic)

^b UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

Author keywords

CCD camera; Computerized processing; gfp expression; Image analysis; Nicotiana tabacum

Indexed keywords

AGROBACTERIUM TUMEFACIENS; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; RADIOBACTER; RHIZOBIUM; TABACUM;

EID: 18144384447     PISSN: 00063134     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10535-005-3316-z     Document Type: Article

References (13)

1. Edwards, K., Johnstone, C., Thompson, C.: A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. - Nucl. Acids Res. 19: 1349, 1991.
2. Halfhill, M.D., Richards, H.A., Mabon, S.A., Stewart, C.N., Jr.: Expression of GFP and Bt transgenes in Brassica napus and hybridization with Brassica rapa. - Theor. appl. Genet. 130: 659-667, 2001.
3. Harper, B.K., Mabon, S.A., Leffel, S.M., Halfhill, M.D., Richards, H.A., Moyer, K.A., Stewart, C.N., Jr.: Green fluorescent protein as a marker for expression of a second gene in transgenic plants. - Nature Biotechnol. 17: 1125-1129, 1999.
4. Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eicholtz, D., Rogers, S.G., Fraley, R.T.: A simple and general method for transferring genes into plants. - Science 227: 1229-1231, 1985.
5. Hu, W., Cheng, C.-L.: Expression of Aequorea green fluorescent protein in plant cells. - FEBS Lett. 369: 331-334, 1995.
6. Maliga, P., Sz.-Breznovits, A., Márton, L.: Streptomycin-resistant plants from callus culture of haploid tobacco. - Nature 244: 29-30, 1973.
7. Miki, B., McHugh, S.: Selectable marker genes in transgenic plants: applications, alternatives and biosafety. - J. Biotechnol. 107: 193-232, 2004.
8. Millwood, R.J., Halfhill, M.D., Harkins, D., Russotti, R., Stewart, C.N., Jr.: Instrumentation and methodology for quantitative GFP fluorescence in intact transgenic plants. - Biotechniques 24: 638-643, 2003.
9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962.
10. Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G., Cormier, M.J.: Primary structure of the Aequorea victoria green-fluorescent protein. - Gene 111: 229-233, 1992.
11. Soukupová, J., Albrechtová, J.: Image analysis - tool for quantification of histochemical detections of phenolic compounds, lignin and peroxidases in neeedles of Norway spruce. - Biol. Plant. 46: 595-601, 2003.
12. Stewart, C.N., Jr.: The utility of green fluorescent protein in transgenic plants. - Plant Cell Rep. 20: 376-382, 2001.
13. Voinnet, O., Baulcombe, D.C.: Systemic signalling in gene silencing. - Nature 389: 553, 1997.