Green fluorescent protein as a marker for expression of a second gene in transgenic plants

Nature Biotechnology

Volumn 17, Issue 11, 1999, Pages 1125-1129

  Harper, Brian K ^a   Mabon, Stephen A ^a   Leffel, Staci M ^a   Halfhill, Matthew D ^a   Richards, Harold A ^a   Moyer, Kari A ^a   Stewart Jr , C Neal ^a  

^a UNIVERSITY OF NORTH CAROLINA AT GREENSBORO   (United States)

Author keywords

Bacillus thuringiensis cry1Ac; Green fluorescent protein; Transgene presence expression; Transgenic plants

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ARTICLE; BACILLUS THURINGIENSIS; BIOASSAY; CROP; GENE EXPRESSION; IMMUNOBLOTTING; NONHUMAN; PHYTOCHEMISTRY; PRIORITY JOURNAL; TRANSGENE; TRANSGENIC PLANT;

BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BRASSICA; ENDOTOXINS; GREEN FLUORESCENT PROTEINS; HEMOLYSIN PROTEINS; LUMINESCENT PROTEINS; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; TOBACCO; TRANSGENES;

EID: 0032692792     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/15114     Document Type: Article

References (26)

1. Raybould, A.F. & Gray, A.J. Genetically modified crops and hybridization with wild relatives: a UK perspective. J. Appl. Ecol. 30, 199-219 (1993).
2. Rogers, H.J. & Parkes, H.C. Transgenic plants and the environment. J.Exp.Bot. 46, 467-488 (1995).
3. Stewart, C.N., Jr. Monitoring transgenic plants using in vivo markers. Nat. Biotechnol. 14, 682 (1996).
4. Heim, R. & Tsien, R.Y. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6, 178-182 (1996).
5. Yang, F., Moss, L.G. & Phillips, G.N., Jr. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14, 1246-1251 (1996).
6. Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G. & Cormier, M.J. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111, 229-233 (1992).
7. Grebenok, R.J. et al. Green-fluorescent protein fusions for efficient characterization of nuclear targeting. Plant J. 11, 573-586 (1997).
8. Kohler, R.H., Zipfel, W.R., Webb, W.W. & Hanson, M.R. The green fluorescent protein as a marker to visualize plant mitochondria in vivo. Plant J. 11, 613-621 (1997).
9. Plautz, J.D. et al. Green fluorescent protein and its derivatives as versatile markers for gene expression in living Drosophila melanogaster, plant and mammalian cells. Gene 173, 83-87 (1996).
10. Haseloff, J., Siemering, K.R., Prasher, D.C. & Hodge, S. Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proc. Natl. Acad. Sci. USA 94, 2122-2127 (1997).
11. Reichel, C. et al. Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono-and dicotyledonous plant cells. Proc. Natl. Acad. Sci. USA 93, 5888-5893 (1996).
12. Siemering, K.R., Golbik, R., Sever, R. & Haseloff, J. Mutations that suppress the thermosensitivity of green fluorescent protein. Curr. Biol. 6, 1653-1663 (1996).
13. Chiu, W.L., Niwa, Y., Zeng, W., Hirano, T., Kobayashi, H. & Sheen, J. Engineered GFP as a vital reporter in plants. Curr. Biol. 6, 325-330 (1996).
14. Haas, J., Park, E.-C. & Seed, B. Codon usage limitation in the expression if HIV-1 envelope glycoprotein. Curr. Biol. 6, 315-324 (1996).
15. An, G. High efficiency transformation of cultured tobacco cells. Plant Physiol. 79, 568-570 (1985).
16. Leffel, S.M., Mabon, S.A. & Stewart, C.N., Jr. Applications of green fluorescent protein in plants. BioTechniques 23, 912-918 (1997).
17. Chevre, A.M., Eber, F., Baranger, A. & Renard, M. Gene flow from transgenic crops. Nature 389, 924-924 (1997).
18. Jørgensen, R.B. & Andersen, B. Spontaneous hybridization between oilseed rape (Brassica napus) and weedy B. campestris (Brassicaceae): a risk of growing genetically modified oilseed rape. Am. J. Bot. 81, 1620-1626 (1994).
19. Mikkelsen, T.R., Andersen, B. & Jørgensen, R.B. The risk of crop transgene spread. Nature 380, 31 (1996).
20. Stewart, C.N., Jr., All, J.N., Raymer, P.L. & Ramachandran, S. Increased fitness of transgenic insecticidal rapeseed under insect selection pressure. Mol. Ecol. 6, 773-779 (1997).
21. Whitton, J., Wolf, D.E., Arias, D.M., Snow, A.A. & Rieseberg, L.H. The persistence of cultivar alleles in wild populations of sunflowers five generations after hybridization. Theor. Appl. Genet. 95, 33-40 (1997).
22. Stewart, C.N., Jr. et al. Genetic transformation, recovery, and characterization of fertile soybean transgenic for a synthetic Bacillus thuringiensis crylAc gene. Plant Physiol. 112, 121-129 (1996).
23. Allen, G.C. et al. High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco. Plant Cell 8, 899-913 (1996).
24. Horsch, R.B. et al. A simple and general method for transferring genes into plants. Science 227, 1229-1231 (1985).
25. Stewart, C.N., Jr. et al. Insect control and dosage effects in transgenic canola, Brassica napus L. (Brassicaceae) containing a synthetic Bacillus thuringiensis crylAc gene. Plant Physiol. 112, 115-120 (1996).
26. Sokal, R.R. & Rohlf, F.J. in Biometry Vol. 2, 372-399 (W.H. Freeman and Company, New York; 1981).