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18044392168
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note
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b in a 1:3 molar ratio was carried out over the pH range 5-12.
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18044393523
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note
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Surface tension measurements were carried out with a drop tensiometer (Tracker; I.T. Concept, Longessagne, France) at 25°C, which allowed the determination of the interfacial tension by analyzing the axial symmetric shape (Laplacian profile) of the rising air drop in solution. The CMC values were estimated from the intersection of the extrapolated linear portions of the plot of surface tension versus ligand or complex concentration.
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17
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18044362216
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note
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The size of the particles was measured by quasielastic light scattering. The movement of particles in suspension is characterized by a diffusion coefficient, and the particles undergo Brownian motion. A Malvern zetasizer 300HsA instrument was used at a scattering angle of 90°. This apparatus includes a 5-mW He-Ne laser that operates at a wavelength of 633 nm. The detector is an avalanche photodiode and the numerical correlator (type 7132CN with 256 channels) is controlled by the AutoSizer software version XY. The sample is contained in a flow-through cell kept at 23°C. The data were analyzed by the CONTIN method. The translational diffusion coefficient is related to particle diameter using Stoke's diffusion law and the Einstein equation for Brownian motion.
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18
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0348170916
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Thin liquid films of the suspensions were fast-frozen in liquid ethane at -171°C with a method described by C. Poncet-Legrand, D. Cartalade, J.-L. Putaux, V. Cheynier, A. Vernhet, Langmuir 2003, 19, 10563. The samples were mounted in a Gatan 626 cryoholder at -180°C, transferred into a Philips CM200 "cryo" transmission electron microscope, and observed under low-dose conditions at low temperature. Images were recorded on Kodak SO163 films.
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19
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18044376523
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note
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[14] The wild-type E. chrysanthemi, a chrysobactin-negative mutant, and a chrysobactin-achromobactin-tonB mutant were used. Iron complex solutions (0.5-50 mM) in culture medium were employed (iron complexes were added at the beginning of iron carency). The experimental protocol for iron nutrition is described in ref. [15].
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