The mammalian homologue of the novel peptide Bv8 is expressed in the central nervous system and supports neuronal survival by activating the MAP kinase/PI-3-kinase pathways

European Journal of Neuroscience

Volumn 13, Issue 9, 2001, Pages 1694-1702

  Melchiorri, D ^a   Bruno, V ^b   Besong, G ^b   Ngomba, R T ^b   Cuomo, L ^b   De Blasi, A ^b,c   Copani, A ^f   Moschella, C ^a   Storto, M ^b   Nicoletti, F ^a,b   Lepperdinger, G ^d   Passarelli, F ^e  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b IRCCS NEUROMED   (Italy)

^c MARIO NEGRI INSTITUTE FOR PHARMACOLOGICAL RESEARCH   (Italy)

^d ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^e SAPIENZA UNIVERSITY OF ROME   (Italy)

^f NONE

Author keywords

Amphibian peptide; Apoptosis; Mouse; Neuroprotection; Rat

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 2 MORPHOLINO 8 PHENYLCHROMONE; BRAIN PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; NEUROTROPHIC FACTOR; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN BV8; RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BRAIN CELL; BRAIN CORTEX; CONTROLLED STUDY; ENZYME ACTIVATION; FROG; GRANULE CELL; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; LIMBIC CORTEX; NERVE CELL; NEUROPROTECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PURKINJE CELL; RAT; SKIN SECRETION; SPINAL CORD DORSAL HORN; SPINAL CORD VENTRAL HORN;

1-PHOSPHATIDYLINOSITOL 3-KINASE; AMPHIBIAN PROTEINS; ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; ASTROCYTES; CELL SURVIVAL; CELLS, CULTURED; CENTRAL NERVOUS SYSTEM; CEREBELLAR CORTEX; CEREBRAL CORTEX; COCULTURE TECHNIQUES; DOSE-RESPONSE RELATIONSHIP, DRUG; EXCITATORY AMINO ACID AGONISTS; IMMUNOHISTOCHEMISTRY; MAP KINASE SIGNALING SYSTEM; N-METHYLASPARTATE; NEURONS; NEUROPEPTIDES; NEUROPROTECTIVE AGENTS; PROTEINS; RATS; RNA, MESSENGER;

EID: 17744392200     PISSN: 0953816X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1460-9568.2001.01549.x     Document Type: Article

References (29)

1. Andersson, I.K., Edwall, D., Norstedt, G., Rozell, B., Skottner, A. & Hansson, H.A. (1988) Differing expression of insulin-like growth factor I in the developing and in the adult rat cerebellum. Acta Physiol. Scand., 132, 167-173.
2. Balazs, R., Gallo, V., Kingbury, A., Thagnipon, W., Smith, R., Atternill, C. & Woodhams, P. (1997) Factors affecting the survival and maturation of nerve cells in culture. In Althaus, H.H., Seifert, W. (eds), Glial-Neuronal Communication in Developmental Regeneration, Springer-Verlag, Berlin, pp. 286-302.
3. Bondeva, T., Pirola, L., Bulgarelli-Leva, G., Rubio, I., Wetzker, R. & Wymann, M.P. (1998) Bifurcation of lipid and protein kinase signals of PI 3Kγ to the protein kinases PKB and MAPK. Science, 282, 293-296.
4. Bondy, C.A. (1991) Transient IGF-I gene expression during the maturation of functionally related projecting neurons. J. Neurosci., 11, 3442-3955.
5. Bonni, A., Brunet, A., West, A.E., Datta, S.R., Takasu, M.A. & Greenberg, M.E. (2000) Cell Survival promoted by ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science, 286, 1358-1362.
6. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding. Anal. Biochem., 7, 248-254.
7. Choi, D.W. (1992) Excitotoxic cell death. J. Neurobiol., 23, 1261-1276.
8. Cobb, M.H. (1999) MAP kinase pathways. Prog. Biophy. Mol. Biol., 71, 479-500.
9. Copani, A., Bruno, V.M.G., Barresi, V., Battaglia, G., Condorelli, D.F. & Nicoletti, F. (1995) Activation of metabotropic glutamate receptors prevents neuronal apoptosis in culture. J. Neurochem., 64, 101-108.
10. D'Mello, S.R., Galli, C., Ciotti, T. & Calissano, P. (1993) Induction of apoptosis in cerebellar granule neurons by low potassium: Inhibition of death by insulin-like growth factor I and cAMP. Proc. Natl. Acad. Sci. USA, 90, 10989-10993.
11. D'Mello, S.R., Borodezt, K. & Soltoff, S.P. (1997) Insulin-like growth factor and potassium depolarization maintain neuronal survival by distinct pathways: possible involvement of PI3-kinase in IGF-I signaling. J. Neurosci., 17, 1548-1560.
12. Dudek, H., Datta, S.R., Franke, T.F., Bimbaum, M.J., Yao, R., Cooper, G.M., Segal, R.A., Kaplan, D.R. & Greenberg, M.E. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science, 275, 661-665.
13. Erspamer, V. (1994) Bioactive secretions of the amphibian integument, In Heatwole, H. (ed.), Amphibian Biology. Surrey Beatty & Sons, Chipping Norton, NSW, pp. 178-350.
14. Gallo, V., Kingsbury, A., Balazs, R. & Jorgeson, O.S. (1987) The role of depolarization in the survival and differentiation of cerebellar granul cells in culture. J. Neurosci., 7, 2202-2213.
15. Herrington, C.S. & McGee, J.O. (1992) Principles and basic methodology of DNA/RNA detection by in situ hybridisation. In Herrington, C.S., McGee, J.O. (eds), Diagnostic Molecular Pathology: a Practical Approach, Vol. 1. IRL Press, New York, NY, pp. 69-102.
16. Joubert, F.J. & Strydom, D.J. (1980) Snake venom. The amino acid sequence of protein A from Dendroaspis polylepsis (black mamba) venom. Hoppe Seylers Z. Phisiol. Chem., 361, 1787-1794.
17. Katsuura, G., Shinohara, S., Shintaku, H., Eigyo, M. & Matsushita, A. (1991) Protective effect of CCK-8 and ceruletide on glutamate-induced neuronal cell death in rat neuron cultures: possible involvement of CCK-B receptors. Neurosci. Lett., 132, 159-162.
18. Lee, J.-M., Zipfel, G.J. & Choi, D.W. (1999) The changing landscape of ischaemic brain injury mechanisms. Nature, 399 (Suppl. 24), A3.
19. Mignogna, G., Simmaco, M., Kreil, G. & Barra, D. (1993) Antibacterial and haemolytic peptides containing D-alloisoleucine fron the skin of Bombina variegata. EMBO J., 12, 4829-4832.
20. Mollay, C., Wechselberger, C., Mignogna, G., Negri, L., Melchiorri, P., Barra, D. & Kreil, G. (1999) Bv8, a small protein from frog skin and its homologue from snake venom induce hyperalgesia in rats. Eur. J. Pharmacol., 374, 189-196.
21. Nicoletti, F., Wroblewski, A., Novelli, A., Alho, A., Guidotti, A. & Costa, E. (1986) The activation of inositol phospholipid metabolism as a signal-transducing system for excitatory amino acids in primary cultures of cerebellar granule cells. J. Neurosci., 6, 1905-1911.
22. Rose, K., Goldberg, M.P. & Choi, D.W. (1992) Cytotoxicity in murine neocortical cell cultures. In Tyson, C.A., Frazier, J.M. (eds), Methods in Toxicology, Vol. 1. San Diego Academics, San Diego, CA, pp. 46-60.
23. Shinohara, S., Katsuura, G., Shinohara, S., Eigyo, M., Shintaku, H., Ibii, N. & Matsushita, A. (1992) Inhibitory effect of CCK-8 and ceruletide on glutamate-induced rises in intracellular free calcium concentrations in rat neuron cultures. Brain Res., 588, 223-228.
24. Simmaco, M., Barra, D., Chiarini, F., Noviello, L., Melchiorri, P., Kreil, G. & Richter, K. (1991) A family of bombin-related peptides from the skin of Bombina variegata. Eur. J. Biochem., 199, 217-222.
25. Snider, B.J., Gottron, F.J. & Choi, D.W. (1999) Apoptosis and necrosis in cerebravascular disease. Ann. N. Y. Acad. Sci., 893, 243-253.
26. Toker, A. & Newton, A.C. (2000) Akt/protein kinase B is regulated by autophosphorylation at the hypothetical PDK-2 site. J. Biol. Chem., 275, 8271-8274.
27. van Tilbeurgh, H., Sarda, L., Verger, R. & Cambillau, C. (1992) Structure of the pancreatic lipase-procolipase complex. Nature, 359, 159-162.
28. Wechselberger, C., Puglisi, R., Engel, E., Lepperdinger, G., Boitani, C. & Kreil, G. (1999) The mammalian homologues of frog Bv8 are mainly expressed in spermatocytes. FEBS Lett., 462, 177-181.
29. Wymann, M.P. & Pirola, L. (1998) Structure and function of phosphoinositide 3-kinases. Biochem. Biophy. Acta. 1436, 127-150.