Expression of lexA targeted ribozyme in Escherichia coli BL-21 (DE3) cells

Molecular and Cellular Biochemistry

Volumn 271, Issue 1-2, 2005, Pages 197-203

  Yadava, Ramesh Singh ^a,b   Kumar, Ravindra ^b,c   Yadava, Pramod Kumar ^b  

^a UNIVERSITY OF VIRGINIA   (United States)

^b JAWAHARLAL NEHRU UNIVERSITY   (India)

^c PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

E. coli; Hammerhead ribozyme; lexA; SOS response

Indexed keywords

HAMMERHEAD RIBOZYME; ISOPROPYL THIOGALACTOSIDE; LEXA PROTEIN; MESSENGER RNA; PROTEINASE; RIBONUCLEASE;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL TRANSFORMATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN TARGETING; RIBONUCLEASE PROTECTION ASSAY; RNA EXTRACTION; RNA ISOLATION;

BACTERIAL PROTEINS; CLONING, MOLECULAR; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; ISOPROPYL THIOGALACTOSIDE; PROMOTER REGIONS (GENETICS); RNA, CATALYTIC; RNA, MESSENGER; SERINE ENDOPEPTIDASES; ULTRAVIOLET RAYS; VIRAL PROTEINS;

ESCHERICHIA COLI; GOES;

EID: 17244365181     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11010-005-6340-6     Document Type: Article

References (27)

1. Zaug AJ, Cech TR: The intervening sequence RNA of Tetrahyme is an enzyme. Science 231: 470-475, 1986
2. Uhlenbeck OC: A small catalytic oligoribonucleotide. Nature 328: 596-600, 1987
3. Haseloff J, Gerlach WL: Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature 334: 585-591, 1988
4. Weinberg M, Passman M, Kew M: Arbuthnot P: Hammerhead ribozyme-mediated inhibition of hepatitis B virus X gene expression in cultured cells. J Hepatol 33: 142-151, 2000
5. Thalle C, et al.: Ribozymes as tools for therapeutic target validation in arthritis. J Immunol 165: 493-498, 2000
6. Kim YK, et al.: Repression of Hepatitis B virus x gene expression by hammerhead ribozymes. Biochem Biophys Res Commun 257: 759-765, 1999
7. Cameron FH, Jennings PA: Specific gene suppression by engineered ribozyme in monkey cells. Proc Natl Acad Sci USA 86: 9139-9143, 1989
8. Cotten M, Birnstiel ML: Ribozyme mediated destruction of RNA in vivo. EMBO J. 8: 3861-3866, 1989
9. Sioud M, Drillica K: Prevention of human immunodeficiency virus type 1 integrase expression in Escherichia coli by a ribozyme. Proc Natl Acad Sci U S A 88: 7303-7307, 1991
10. Atkins D, Patrikakis M, Izant JG: The ade6 gene of the fission yeast as a target for antisense and ribozyme RNA-mediated suppression. Antisense Res Dev 5:295-305, 1995
11. Sarver N, Cantin EM, Chang PS, Zaia JA, Ladne PA, Stephens DA, Rossi JJ: Ribozyme as potential anti-HIV-1 therapeutic agents. Science 247: 1222-1225, 1990
12. Weerasinghe M, Liem SE, Asad S, Read SE, Joshi S: Resistance to human immunodeficiency virus type-1(HIV-1) infection in CD4 + lymphocyte derived cell lines conferred by using retroviral vectors expressing an HIV-1 RNA specific ribozyme. J Virol 65: 5531-5534, 1991
13. Dropulic B, Lin NH, Martin MA, Jeang KT: Functional characterization of a U5 ribozyme: Intracellular suppression of human immunodeficiency virus type 1 expression. J Virol 66(3): 1432-1441, 1992
14. Altman S: RNA enzyme directed gene therapy. Proc Natl Acad Sci USA 90: 10898-10900, 1993
15. Chuat JC, Galibert F: Can ribozymes be used to regulate procaryote gene expression? Biochem Biophys Res Commun 162(3): 1025-1029, 1989
16. Winterling KW, Levine A, Yasbin R, Woodgate R: Characterization of DinR, the Bacillus subtilis SOS Repressor. J Bact 179: 1698-1703, 1997
17. Little JW, Harper JE: Identification of the lexA gene product of Escherichia coli K-12. Proc Natl Acad Sci USA 76: 6147-6151, 1979
18. Little JW, Mount DW: The SOS regulatory system of Escherichia coli. Cell 29: 11-22, 1982
19. Little JW: LexA Cleavage and other self-processing reactions. J Bact 175: 4943-4950, 1993
20. Miller C, Thomsen LE, Gaggero C, Mosseri R, Ingmer H, Cohen SN: SOS response induction by b-actams and bacterial defense against antibiotic lethality. Science 305: 1629-1631, 2004
21. Balaban NQ, Merrin J, Chait R, Kowalik L, Leibler S: Bacterial persistence as a phenotypic switch. Science 305: 1622-1625, 2004
22. Levin BR: Noninherited resistance to anitbiotics. Science 1578-1579, 2004
23. Chen H, Ferbeyre G, Cedergren R: Efficient hammerhead ribozyme and antisense RNA targeting in a slow ribosome Escherichia coli mutant. Nat Biotechnolgy 15: 432-435, 1997
24. Kim KS, Febbraio M, Han TH, Wessel TC, Park DH, Joh TH: Gene probes: A practical approach. In: B.D. Hames, S.J. Higgins (eds). IRL press, Chap 7, 1995 pp 151-182
25. Zuker M: On finding all suboptimal foldings of an RNA molecule. Science 244: 48-52 1989 1:CAS:528:DyaL1MXkt1SnsbY%3D
26. Young MBD, Denker JR, Boehm CA, Riek RP, Mamone JA, McSwiggen JA, Graham RM: Functional characterization of ribozymes expressed using U1 and T7 vectors for the intracellular cleavage of ANF mRNA. Biochemistry 33: 12127-12138 1994
27. Wang JY, Qiu L, Wu ED, Darlica K: RNas involved in ribozyme degradation in Escherichia coli. J Bact 178: 1640-1645, 1996