Improvements in histological quality and signal retention following in situ hybridization in early chick embryos using plastic resin and recolorization

Biotechnic and Histochemistry

Volumn 80, Issue 1, 2005, Pages 35-41

  Sawitzke, A L ^a,b   Chapman, S C ^b   Bleyl, S B ^b   Schoenwolf, Gary C ^b  

^a SALT LAKE COMMUNITY COLLEGE   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

Alkaline phosphatase; Chick embryos; Digoxigenin; Gene expression; Histology; In situ hybridization; NBT BCIP; Plastic embedding; Whole mounts

Indexed keywords

AGAROSE; ALKALINE PHOSPHATASE; ANTIBODY CONJUGATE; CHORDIN; DIGOXIGENIN; FIBROBLAST GROWTH FACTOR 8; GELATIN; NITROBLUE TETRAZOLIUM; PARAFFIN; PLASTIC; PLATELET DERIVED GROWTH FACTOR A; RESIN; TRANSCRIPTION FACTOR PAX2;

ACCURACY; ANIMAL TISSUE; ARTICLE; CHEMICAL REACTION; CHICK EMBRYO; COLOR; CONTROLLED STUDY; CULTURE MEDIUM; EMBEDDING; EMBRYO; GENE EXPRESSION; HISTOLOGY; IN SITU HYBRIDIZATION; NONHUMAN; PRECIPITATION; QUALITY CONTROL; RELIABILITY; SIGNAL DETECTION; TISSUE SECTION;

ALKALINE PHOSPHATASE; ANIMALS; CHICK EMBRYO; DIGOXIGENIN; HISTOLOGICAL TECHNIQUES; HISTOLOGY; IN SITU HYBRIDIZATION; MOLECULAR PROBES; PLASMIDS; PLASTIC EMBEDDING; PLASTICS; RESINS, PLANT; SENSITIVITY AND SPECIFICITY; TETRAZOLIUM SALTS; TRANSCRIPTION FACTORS;

EID: 17144424631     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.1080/10520290500050957     Document Type: Article

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