High-efficiency protein extraction from polyacrylamide gels for molecular mass measurement by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

Electrophoresis

Volumn 26, Issue 6, 2005, Pages 1019-1028

  Jin, Ya ^a   Manabe, Takashi ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Ionization time of flight mass spectrometry; Matrix assisted laser desorption; Molecular mass measurement; Polyacrylamide gel electrophoresis; Protein extraction

Indexed keywords

CARBONIC ANHYDRASE; DESORPTION; ELECTROPHORESIS; EXTRACTION; INDUCTIVELY COUPLED PLASMA; MASS SPECTROMETERS; MASS SPECTROMETRY; MOLECULAR MASS; NEGATIVE IONS; SODIUM HYDROXIDE;

COOMASSIE BRILLIANT BLUE; HIGHER EFFICIENCY; IONIZATION TIME OF FLIGHT MASS SPECTROMETRIES; MASS MEASUREMENTS; MATRIX ASSISTED LASER DESORPTION; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; MOLECULAR MASS MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYACRYLAMIDE GELS; PROTEIN EXTRACTION;

GELS;

ALBUMIN; ALPHA LACTALBUMIN; ANAZOLENE SODIUM; BETA LACTOGLOBULIN; BRILLIANT BLUE; CARBONATE DEHYDRATASE; CYTOCHROME C; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MYOGLOBIN; POLYACRYLAMIDE GEL; PROTEIN; TRYPSINOGEN;

ARTICLE; EXTRACTION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; PROTEIN PURIFICATION;

EID: 17144399857     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200410187     Document Type: Article

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