Reverse zymography using fluorogenic substrates for protease inhibitor detection

Electrophoresis

Volumn 26, Issue 6, 2005, Pages 1038-1045

  Le, Quang T ^a,b   Ohashi, Atsushi ^a   Hirose, Sayu ^a   Katunuma, Nobuhiko ^a  

^a TOKUSHIMA BUNRI UNIVERSITY   (Japan)

^b VIETNAM NATIONAL UNIVERSITY   (Viet Nam)

Author keywords

Fluorogenic substrate; Protease inhibitors; Reverse zymography

Indexed keywords

ELECTROPHORESIS; FLUORESCENCE; PEPTIDES; SODIUM DODECYL SULFATE; SUBSTRATES; SULFUR COMPOUNDS;

FLUOROGENIC SUBSTRATE; PEPTIDE SUBSTRATES; POLYACRYLAMIDE GELS; PROTEASE INHIBITOR; REVERSE ZYMOGRAPHY; SENSITIVE METHOD; SHORT PERIODS; SODIUM DODECYL SULPHATE; TRANSILLUMINATORS; ZYMOGRAPHY;

GELS;

4 METHYLCOUMARYL 7 AMIDE; AMINO ACID DERIVATIVE; APROTININ; BENZYLOXYCARBONYLPHENYLALANYLARGININE 4 METHYLCOUMARYL 7 AMIDE; CHYMOTRYPSIN A; CHYMOTRYPSIN INHIBITOR; COUMARIN DERIVATIVE; CYSTATIN C; FLUORESCENT DYE; PAPAIN; POLYPEPTIDE; PROTEINASE; PROTEINASE INHIBITOR; SERINE PROTEINASE INHIBITOR; SOYBEAN TRYPSIN INHIBITOR; SUCCINYLALANYLALANYLPROLYLPHENYLALANINE 4 METHYLCOUMARYL 7 AMIDE; TERT BUTYLOXYCARBONYLPHENYLALANYLSERYLARGININE 4 METHYLCOUMARYL 7 AMIDE; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; DRUG DETERMINATION; DRUG DISCRIMINATION; ELECTROPHORESIS; ENZYMATIC ASSAY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FLUORESCENCE; PROTEIN DEGRADATION; REVERSE ZYMOGRAPHY; ZYMOGRAPHY;

EID: 17144370192     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200306142     Document Type: Article

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