Two-stage off-gel™ isoelectric focusing: Protein followed by peptide fractionation and application to proteome analysis of human plasma

Electrophoresis

Volumn 26, Issue 6, 2005, Pages 1174-1188

  Heller, Manfred ^a   Michel, Philippe E ^a   Morier, Patrick ^a   Crettaz, David ^b   Wenz, Christian ^c   Tissot, Jean Daniel ^b   Reymond, Frédéric ^a   Rossier, Joel S ^a  

^a DiagnoSwiss SA   (Switzerland)

^b Service Regional Vaudois de Transfusion Sanguine   (Switzerland)

^c AGILENT TECHNOLOGIES   (Germany)

Author keywords

Isoelectric focusing; Off gel electrophoresis; Peptides; Proteomics

Indexed keywords

BLOOD; ELECTROPHORESIS; FRACTIONATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PHYSICOCHEMICAL PROPERTIES;

GEL ELECTROPHORESIS; GEL ISOELECTRIC FOCUSING; HUMAN PLASMAS; INTACT PROTEINS; ISOELECTRIC FOCUSING; OFF-GEL ELECTROPHORESIS; PROTEIN FRACTIONS; PROTEOME ANALYSIS; PROTEOMICS; REVERSE-PHASE LIQUID CHROMATOGRAPHY;

PEPTIDES;

ALBUMIN; ALPHA 1 ANTITRYPSIN; HAPTOGLOBIN; IMMUNOGLOBULIN; IMMUNOGLOBULIN A; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN G; PEPTIDE; PLASMA PROTEIN; PROTEIN; TRANSFERRIN;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; ARTICLE; HUMAN; ISOELECTRIC FOCUSING; OFF GEL ELECTROPHORESIS; PLASMA; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEOMICS; REPRODUCIBILITY; REVERSED PHASE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY; UNINDEXED SEQUENCE;

EID: 17144362794     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200410106     Document Type: Article

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