The molybdenum cofactor biosynthesis protein MobA from Rhodobacter capsulatus is required for the activity of molybdenum enzymes containing MGD, but not for xanthine dehydrogenase harboring the MPT cofactor

FEMS Microbiology Letters

Volumn 174, Issue 2, 1999, Pages 239-246

  Leimkühler, Silke ^a,b   Klipp, Werner ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

HMP, hypoxanthine monophosphate; MGD, molybdopterin guanine dinucleotide; mob locus; Molybdenum cofactor biosynthesis; Molybdoenzyme; Molybdopterin guanine dinucleotide synthase; MPT, molybdopterin; Rhodobacter capsulatus

Indexed keywords

DIMETHYL SULFOXIDE; MOLYBDENUM; MOLYBDENUM ENZYME; MOLYBDOPTERIN GUANINE DINUCLEOTIDE SYNTHASE; NITRATE REDUCTASE; OXIDOREDUCTASE; UNCLASSIFIED DRUG; XANTHINE DEHYDROGENASE;

ARTICLE; BIOSYNTHESIS; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE LOCUS; GENE MUTATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RHODOBACTER CAPSULATUS;

ESCHERICHIA COLI; RHODOBACTER CAPSULATUS; RHODOBACTER SPHAEROIDES;

EID: 16744365314     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00147-0     Document Type: Article

References (28)

1. Blattner, F.R. et al. (1997) The complete genome sequence of Escherichia coli K-12. Science 277, 1453-1462.
2. Börner, G., Karrasch, M. and Thauer, R.K. (1991) Molybdopterin adenine dinucleotide and molybdopterin hypoxanthine dinucleotide in formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum (Marburg). FEBS Lett. 290, 31-34.
3. Bull, C.J. et al. (1996) Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273, 1058-1073.
4. Colonna-Romano, S., Arnold, W., Schlüter, A., Boistard, P., Pühler, A. and Priefer, U.B. (1990) An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK. Mol. Gen. Genet. 223, 138-147.
5. Eaves, D.J., Palmer, T. and Boxer, D.H. (1997) The product of the molybdenum cofactor gene mobB of Escherichia coli is a GTP-binding protein. Eur. J. Biochem. 246, 690-697.
6. Fleischmann, R.D. et al. (1995) Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269, 496-512.
7. Garrett, R.H. and Nason, A. (1969) Further purification and properties of Neurospora nitrate reductase. J. Biol. Chem. 244, 2870-2882.
8. Glaser, J.H. and DeMoss, J.A. (1972) Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol. Gen. Genet. 116, 1-10.
9. Iobbi-Nivol, C., Palmer, T., Whitty, P.W., McNairn, E. and Boxer, D.H. (1995) The mob locus of Escherichia coli K12 required for molybdenum cofactor biosynthesis is expressed at very low levels. Microbiology 141, 1663-1671.
10. Kaneko, T. et al. (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3, 109-136.
11. Klipp, W., Masepohl, B. and Pühler, A. (1988) Identification and mapping of nitrogen fixation genes of Rhodobacter capsulatus: duplication of a nifA-nifB region. J. Bacteriol. 170, 693-699.
12. Labes, M., Pühler, A. and Simon, R. (1990) A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for Gram-negative bacteria. Gene 89, 37-46.
13. Leimkühler, S., Kern, M., Solomon, P.S., McEwan, A.G., Schwarz, G., Mendel, R.R. and Klipp, W. (1998) Xanthine dehydrogenase from the phototrophic purple bacterium Rhodobacter capsulatus is more similar to its eukaryotic counterparts than to prokaryotic molybdenum enzymes. Mol. Microbiol. 27, 853-869.
14. McEwan, A.G., Wetzstein, H.G., Ferguson, S.J. and Jackson, J.B. (1985) Periplasmic location of the terminal reductase in trimethylamine N-oxide and dimethylsulfoxide respiration in the photosynthetic bacterium Rhodopseudomonas capsulata. Biochim. Biophys. Acta 806, 410-417.
15. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
16. Palmer, T., Vasishta, A., Whitty, P.W. and Boxer, D.H. (1994) Isolation of protein FA, a product of the mob locus required for molybdenum cofactor biosynthesis in Escherichia coli. Eur. J. Biochem. 222, 687-692.
17. Palmer, T., Santini, C.-L., Iobbi-Nivol, C., Eaves, D.J., Boxer, D.H. and Giordano, G. (1996) Involvement of the narJ and mob gene products in distinct steps in the biosynthesis of the molybdoenzyme nitrate reductase in Escherichia coli. Mol. Microbiol. 20, 875-884.
18. Palmer, T., Goodfellow, I.P.G., Sockett, R.E., McEwan, A.G. and Boxer, D.H. (1998) Characterization of the mob locus from Rhodobacter sphaeroides required for molybdenum cofactor biosynthesis. Biochim. Biophys. Acta 1395, 135-140.
19. Rajagopalan, K.V. and Johnson, J.L. (1992) The pterin molybdenum cofactors. J. Biol. Chem. 267, 10199-10202.
20. Reyes, F., Roldan, M.D., Klipp, W., Castillo, F. and Moreno-Vivian, C. (1996) Isolation of periplasmic nitrate reductase genes from Rhodobacter sphaeroides DSM 158: structural and functional differences among prokaryotic nitrate reductases. Mol. Microbiol. 19, 1307-1318.
21. Rivers, S.L., McNairn, E., Blasco, F., Giordano, G. and Boxer, D.H. (1993) Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis. Mol. Microbiol. 8, 1071-1081.
22. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
23. Schneider, F., Löwe, J., Huber, R., Schindelin, H., Kisker, C. and Knäblein, J. (1996) Crystal structure of dimethyl sulfoxide reductase from Rhodobacter capsulatus at 1.88 Å resolution. J. Mol. Biol. 263, 53-69.
24. Shaw, A.L., Hanson, G.R. and McEwan, A.G. (1996) Cloning and sequence analysis of the dimethylsulfoxide reductase structural gene from Rhodobacter capsulatus. Biochim. Biophys. Acta 1276, 176-180.
25. Simon, R., Priefer, U. and Pühler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. BioTechnology 1, 784-791.
26. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J. and Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76-85.
27. Vieira, J. and Messing, J. (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259-268.
28. Wang, G., Angermüller, S. and Klipp, W. (1993) Characterization of Rhodobacter capsulatus genes encoding a molybdenum transport system and putative molybdenum-pterin-binding proteins. J. Bacteriol. 175, 3031-3042.