Tetracycline-regulated enables purification and functional analysis of recombinant connexin channels from mammalian cells

Biochemical Journal

Volumn 383, Issue 1, 2004, Pages 111-119

  Koreen, Irina V ^a   Elsayed, Wafaa A ^a   Liu, Yu J ^a   Harris, Andrew L ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

Connexin; Gap junction; Hemichannel; Inducible expression; Permeability; Purification

Indexed keywords

CELL CULTURE; CLONING; PROTEINS; SYNCHRONIZATION; TISSUE;

CELLULAR DIFFERENTIATION; GAP JUNCTION CHANNELS; MOLECULAR PERMEABILITY; TISSUE SYNCHRONIZATION;

CELLS;

CONNEXIN 26; CONNEXIN 32; DOXYCYCLINE; GAP JUNCTION PROTEIN; HEMAGGLUTININ; ION CHANNEL; ISOPROTEIN; RECOMBINANT CONNEXIN; TETRACYCLINE; UNCLASSIFIED DRUG; INOSITOL 1,4,5 TRISPHOSPHATE; MULTIPROTEIN COMPLEX; RECOMBINANT PROTEIN; VIRUS HEMAGGLUTININ;

AMINO ACID COMPOSITION; ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; CELL CULTURE; CELL DIFFERENTIATION; CELL METABOLISM; CELL SYNCHRONIZATION; CONTROLLED STUDY; CYTOPLASM; GAP JUNCTION; GENE EXPRESSION; GENE EXPRESSION REGULATION; HELA CELL; HOMEOSTASIS; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MAMMAL CELL; MEMBRANE PERMEABILITY; MOLECULAR CLONING; MOLECULAR DYNAMICS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING; BIOSYNTHESIS; CELL JUNCTION; CHEMISTRY; DRUG EFFECT; GENE VECTOR; GENETIC TRANSFECTION; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PERMEABILITY; PHYSIOLOGY; SYNTHESIS; TRANSPORT AT THE CELLULAR LEVEL;

MAMMALIA;

BASE SEQUENCE; BIOLOGICAL TRANSPORT; CONNEXINS; DOXYCYCLINE; GAP JUNCTIONS; GENE EXPRESSION REGULATION; GENETIC VECTORS; HELA CELLS; HEMAGGLUTININS, VIRAL; HUMANS; INOSITOL 1,4,5-TRISPHOSPHATE; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PERMEABILITY; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 16644392770     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040806     Document Type: Article

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