Effective non-viral leader for cap-independent translation in a eukaryotic cell-free system

Biotechnology and Bioengineering

Volumn 88, Issue 6, 2004, Pages 730-739

  Shaloiko, L A ^a,b   Granovsky, I E ^c   Ivashina, T V ^a,c   Ksenzenko, V N ^a   Shirokov, V A ^a   Spirin, A S ^a  

^a INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

^b SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^c SKRYABIN INSTITUTE OF BIOCHEMISTRY AND PHYSIOLOGY OF MICROORGANISMS   (Russian Federation)

Author keywords

5 UTR obelin mRNA leader; Cap independent translation; Cell free translation systems; Sequestration of RNA binding translation factors; Translation inhibition by excess mRNA

Indexed keywords

CATALYSIS; CHELATION; ENZYMES; PROTEINS; RNA;

LIGHT-EMITTING PROTEINS; OBELIN; SEQUESTRATION; TRANSLATION SYSTEMS;

CELLS;

INITIATION FACTOR; INITIATION FACTOR 4F; MESSENGER RNA; OBELIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; CAPPED RNA; PHOTOPROTEIN; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CAP INDEPENDENT TRANSLATION; CELL FREE SYSTEM; CONTROLLED STUDY; EUKARYOTE EVOLUTION; GENE CONSTRUCT; NONHUMAN; NUCLEOTIDE SEQUENCE; OBELIA; OBELIA LONGISSIMA; RNA TRANSLATION; TRANSCRIPTION REGULATION; BIOSYNTHESIS; EUKARYOTIC CELL; GENE VECTOR; GENETICS; METABOLISM; METHODOLOGY; PHYSIOLOGY; PRENATAL DEVELOPMENT; PROTEIN ENGINEERING; PROTEIN SYNTHESIS; VIRUS; WHEAT;

EUKARYOTA; OBELIA LONGISSIMA; TRITICUM AESTIVUM;

5' UNTRANSLATED REGIONS; CELL-FREE SYSTEM; EUKARYOTIC CELLS; GENETIC VECTORS; LUMINESCENT PROTEINS; PLANT PROTEINS; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA CAP ANALOGS; TRITICUM; VIRUSES;

EID: 16644362175     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20267     Document Type: Article

References (53)

1. Belsham GJ, Sonenberg N. 2000. Picornavirus RNA translation: Roles for cellular proteins. Trends Microbiol 8:330-335.
2. Browning KS, Hamphreys J, Hobbs W, Smith GB, Ravel JM. 1990. Determination of the amounts of the protein synthesis initiation and elongation factors in wheat germ. J Biol Chem 265:17967-17973.
3. Carrington JC, Freed DD. 1990. Cap-independent enhancement of translation by a plant potyvirus 5′ nontranslated region. J Virol 64:1590-1597.
4. Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
5. Danthinne X, Seurinck J, Meulewaeter F, van Montagu M, Cornelissen M. 1993. The 3′ untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro. Mol Cell Biol 13:3340-3349.
6. Duncan R, Hershey JWB. 1983. Identification and quantitation of levels of protein synthesis initiation factors in crude HeLa cell lysates by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem 258:7228-7235.
7. Duncan R, Milburn SC, Hershey JWB. 1987. Regulated phoshorylation and low abundance of HeLa initiation factor eIF4-4F suggest a role in translational control. J Biol Chem 262:380-388.
8. Erickson AH, Blobel G. 1983. Cell-free translation of messenger RNA in a wheat germ system. Methods Enzymol 96:38-50.
9. 7G cap on β-globin mRNA and alfalfa mosaic virus RNA 4 increases the amounts of initiation factor 4F required for initiation. J Biol Chem 265:19582-19587.
10. Florentz C, Giegé R. 1995. tRNA-like structures in plant viral RNAs. In: Söll D, RajBhandary UL, editors. tRNA. Structure, biosynthesis, and function. Washington, DC: ASM Press, p 141-163.
11. Gallie DR. 2001. Cap-independent translation conferred by the 5′ leader of tobacco etch virus is eukaryotic initiation factor 4G dependent. J Virol 75:12141-12152.
12. Gallie DR. 2002. The 5′-leader of tobacco mosaic virus promotes translation through enhanced recruitment of eIF4F. Nucleic Acids Res 30:3401-3411.
13. Gallie DR, Walbot V. 1990. RNA preudoknot domain of tobacco mosaic virus can functionally substitute for a poly(A) tail in plant and animal cells. Genes Dev 4:1149-1157.
14. Gallie DR, Feder JN, Schimke RT, Walbot V. 1991. Functional analysis of the tobacco mosaic virus tRNA-like structure in cytoplasmic gene regulation. Nucleic Acids Res 19:5031-5036.
15. Gallie DR, Tanguay RL, Leathers V. 1995. The tobacco etch viral 5′ leader and poly(A) tail are functionally synergistic regulators of translation. Gene 165:233-238.
16. Gazo BM, Murphy P, Gatchel JR, Browning KS. 2004. A novel interaction of cap-binding complexes eIF4F and eIF(iso)4F with a region in the 3′ untranslated region of Satellite Tobacco Necrosis Virus. J Biol Chem 279:13584-13592.
17. Guo L, Allen EM, Miller WA. 2001. Base-pairing between untranslated regions facilitates translation of uncapped, nonpolyadenylated viral RNA. Mol Cell 7:1103-1109.
18. Haenni A-L, Joshi S, Chapeville F. 1982. tRNA-like structures in the genomes of RNA viruses. Prog Nucleic Acid Res Mol Biol 27:85-104.
19. Hellen CU, Sarnow P. 2001. Internal ribosome entry sites in eukaryotic mRNA molecules. Genes Dev 15:1593-1612.
20. Hiremath LS, Webb NR, Rhoads RE. 1985. Immunological detection of the messenger RNA cap-binding protein. J Biol Chem 260:7843-7849.
21. Imataka H, Gradi A, Sonenberg N. 1998. A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation. EMBO J 17:7480-7489.
22. Jackson RJ. 2000. A comparative view of initiation site selection mechanisms. In: Sonenberg N, Hershey JWB, Mathews MB, editors. Translational control of gene expression. New York: CSHL Press, p 127-183.
23. Jackson RJ, Hunt SL, Gibbs CL, Kaminski A. 1994. Internal initiation of translation of picornavirus RNAs. Mol Biol Reports 19:147-159.
24. Jackson RJ, Hunt SL, Reynolds JE, Kaminski A. 1995. Cap-dependent and cap-independent translation: Operational distinctions and mechanistic interpretations. In: Sarnow P, editor. Cap-independent translation. Topics in microbiology and immunology, vol. 203. Berlin: Springer-Verlag, p 1-29.
25. Kawarasaki Y, Kasahara S, Kodera N, Shinbata T, Sekiguchi S, Nakano H, Yamane T. 2000. A trimmed viral cap-independent translation enhancing sequence for rapid in vitro gene expression. Biothechnol Prog 16:517-521.
26. Kolupaeva VG, Pestova TV, Hellen CU, Shatsky IN. 1998. Translation eukaryotic initiation factor 4G recognizes a specific structural element within the internal ribosome entry site of encephalomyocarditis virus RNA. J Biol Chem 273:18599-18604.
27. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
28. Le H, Browning KS, Gallic DR. 2000. The phosphorylation state of poly(A)-binding protein specifies its binding to poly(A) RNA and its interaction with eukaryotic initiation factor (eIF) 4F, eIFiso4F, and eIF4B. J Biol Chem 275:17452-17462.
29. Le H, Tanguay RL, Balasta ML, Wei C-C, Browning KS, Metz AM, Goss DJ, Gallie DR. 1997. Translation initiation factors eIF-iso4G and eIF-4B interact with the poly(A)-binding protein and increase its RNA-binding activity. J Biol Chem 272:16247-16255.
30. Ling J, Morley SJ, Pain VM, Marzluff WF, Gallie DR. 2002. The histone 3′-terminal stem-loop-binding protein enhances translation through a functional and physical interaction with eukaryotic initiation factor 4G (eIF4G) and eIF3. Mol Cell Biol 22:7853-7867.
31. Madin K, Sawasaki T, Ogasawara T, Endo Y. 2000. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes. Proc Natl Acad Sci USA 97:559-564.
32. Mans RMV, Pleij CWA, Bosch L. 1991. tRNA-like structures. Structure, function and evolutionary significance. Eur J Biochem 201:303-324.
33. Matveev SV, Illarionov BA, Vysotski ES, Bondar VS, Markova SV, Alakhov YB. 1995. Obelin mRNA-A new tool for studies of translation in cell-free systems. Anal Biochem 231:34-39.
34. Meulewaeter F, Danthinne X, van Montagu M, Cornelissen M. 1998a. 5′- and 3′-sequences of satellite tobacco necrosis virus RNA promoting translation in tobacco. The Plant Journal 14:169-176.
35. Meulewaeter F, van Montagu M, Cornelissen M. 1998b. Features of the autonomous function of the translational enhancer domain of satellite tobacco necrosis virus. RNA 4:1347-1356.
36. Pestova TV, Kolupaeva VG. 2002. The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selection. Genes Dev 16:2906-2922.
37. Pestova TV, Hellen CU, Shatsky IN. 1996a. Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry. Mol Cell Biol 16:6859-6869.
38. Pestova TV, Hellen CU, Shatsky IN. 1996b. Functional dissection of eukaryotic initiation factor 4F: The 4A subunit and the central domain of the 4G subunit are sufficient to mediate internal entry 43S preinitiation complexes. Mol Cell Biol 16:6870-6878.
39. Pokrovskaya ID, Gurevich VV. 1994. In vitro transcription: Preparative RNA yields in analytical reactions. Anal Biochem 220:420-423.
40. Rhoads RE. 1991. Initiation: mRNA and 60S subunit binding. In: Trachsel H, editor. Translation in eukaryotes. Boca Raton: CRC Press, p 109-148.
41. Ryabova LA, Torgashov AF, Kurnasov OV, Bubunenko MG, Spirin AS. 1993. The 3′-terminal region of alfalfa mosaic virus RNA4 facilitates the RNA entry into translation in a cell-free system. FEBS Letters 326:264-266.
42. Shirokov VA, Simonenko PN, Biryukov SA, Spirin AS. 2002. Continuous-flow and continuous-exchange cell-free translation systems and reactors. In: Spirin AS, editor. Cell-free translation systems. Berlin: Springer-Verlag, p 91-107.
43. Spirin AS. 2002. Cell-free protein synthesis. In: Spirin AS, editor. Cell-free translation systems. Berlin: Springer-Verlag, p 3-20.
44. Spirin AS, Baranov VI, Ryabova LA, Ovodov SY, Alakhov YB. 1988. A continuous cell-free translation system capable of producing polypeptides in high yield. Science 242:1162-1164.
45. Tarun SZ, Sachs AB. 1996. Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G. EMBO J 15:7168-7177.
46. Timmer RT, Benkowski LA, Schodin D, Lax SR, Metz AM, Ravel JM, Browning KS. 1993. The 5′ and 3′ untranslated regions of satellite tobacco necrosis virus RNA affect translational efficiency and dependence on a 5′ cap structure. J Biol Chem 268:9504-9510.
47. Vagner S, Galy B, Pyronnet S. 2001. Irresistible IRES: Attracting the translation machinery to internal ribosome entry sites. EMBO Reports 2:893-898.
48. Van Lipzig R, van Montagu M, Cornelissen M, Meulewaeter F. 2001. Functionality of the STNV translational enhancer domain correlates with affinity for two wheat germ factors. Nucl Acids Res 29:1080-1086.
49. Walden WE, Godefroy-Colburn T, Thach RE. 1981. The role of mRNA competition in regulating translation. J Biol Chem 256:11739-11746.
50. Wang S, Miller WA. 1995. A sequence located 4.5 to 5 kilobases from the 5′-end of the barley yellow dwarf virus (PAV) genome strongly stimulates translation of uncapped mRNA. J Biol Chem 270:13446-13452.
51. Wang S, Browning KS, Miller WA. 1997. A viral sequence in the 3′-untranslated region mimics a 5′ cap in facilitating translation of uncapped mRNA. EMBO J 16:4107-4116.
52. Wang S, Guo L, Allen E, Miller WA. 1999. A potential mechanism for selective control of cap-independent translation by a viral RNA sequence in cis and in trans. RNA 5:728-738.
53. Zeyenko VV, Ryabova LA, Gallie DR, Spirin AS. 1994. Enhancing effect of the 3′-untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro. FEBS Letters 354:271-273.