Separate control of rep and cap expression using mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 27, Issue 1, 2004, Pages 7-14

  Mizukami, Hiroaki ^b   Okada, Takashi ^b   Ogasawara, Yoji ^b   Matsushita, Takashi ^b   Urabe, Masashi ^b   Kume, Akihiro ^b   Ozawa, Keiya ^a  

^a JICHI MEDICAL UNIVERSITY   (Japan)

^b NONE

Author keywords

293 cells; AAV vector; Cre loxP; Dependovirus; Mutant loxP; Packaging cell line

Indexed keywords

CELLS; ENZYMES; MATHEMATICAL MODELS; MUTAGENS; SET THEORY;

293 CELLS; ADENO-ASSOCIATED VIRUS (AAV) VECTOR; CRE-LOXP; DEPENDOVIRUS; MUTANT LOXP; PACKAGING CELL LINE;

VIRUSES;

BETA GALACTOSIDASE; CRE RECOMBINASE; HELICASE; PARVOVIRUS VECTOR; PLASMID VECTOR; PROTEIN; PROTEIN CAP; UNCLASSIFIED DRUG; VIRUS PROTEIN; CELL SURFACE RECEPTOR; CRP PROTEIN, E COLI; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; INTEGRASE; REP PROTEINS, ADENO ASSOCIATED VIRUS 2; REP PROTEINS, ADENO-ASSOCIATED VIRUS 2; TRANSCRIPTION FACTOR;

ARTICLE; GENE EXPRESSION; GENE TRANSFER; GENOME; HUMAN; HUMAN CELL; LOXP SITE; NONHUMAN; PROTEIN EXPRESSION; STATISTICAL SIGNIFICANCE; TITRIMETRY; WILD TYPE; ADENO ASSOCIATED VIRUS; CELL LINE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; MOLECULAR BIOLOGY; MUTATION; VIROLOGY;

ADENO-ASSOCIATED VIRUS; DEPENDOVIRUS; PARVOVIRUS;

CELL LINE; DEPENDOVIRUS; DNA-BINDING PROTEINS; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, VIRAL; GENETIC VECTORS; HUMANS; INTEGRASES; MOLECULAR BIOLOGY; MUTATION; RECEPTORS, CELL SURFACE; RECOMBINATION, GENETIC; TRANSCRIPTION FACTORS; VIRAL PROTEINS; VIROLOGY;

EID: 16544389570     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/mb:27:1:07     Document Type: Article

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