Effect of specific mutations in helix α7 of domain I on the stability and crystallization of Cry3A in Bacillus thuringiensis

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 27, Issue 2, 2004, Pages 89-100

  Park, Hyun Woo ^a   Federici, Brian A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bacillus thuringiensis; Cry1C; Cry3A; Crystallization; Domain substitution; Site directed mutagenesis

Indexed keywords

AMINO ACIDS; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; CRYSTALLIZATION; INCLUSIONS; INSECT CONTROL; MUTAGENESIS; SYNTHESIS (CHEMICAL);

BACILLUS THURINGINESIS; CELL LYSIS; CRYIC; DOMAIN SUBSTITUTION; INSECTICIDAL CRYSTAL (CRY) PROTEINS; SITE-DIRECTED MUTAGENESIS;

MOLECULAR BIOLOGY;

BACTERIAL PROTEIN; CRYSTAL PROTEIN 3A; TYROSINE; UNCLASSIFIED DRUG; BACTERIAL TOXIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; PROTEIN; TRYPSIN;

ALPHA HELIX; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL STRAIN; CONTROLLED STUDY; CRYSTALLIZATION; MOLECULAR WEIGHT; MUTATION; NONHUMAN; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; BIOTECHNOLOGY; CHEMISTRY; CIRCULAR DICHROISM; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTAGENESIS; PHASE CONTRAST MICROSCOPY; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SCANNING ELECTRON MICROSCOPY; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

BACILLUS (BACTERIUM); BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); INSECTA;

AMINO ACID SEQUENCE; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BIOTECHNOLOGY; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; HEMOLYSIN PROTEINS; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, PHASE-CONTRAST; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRYPSIN; TYROSINE;

EID: 16544374775     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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