Optimized expression and refolding of human keratoepithelin in BL21 (DE3)

Protein Expression and Purification

Volumn 35, Issue 1, 2004, Pages 39-45

  Yuan, Ching ^a   Reuland, Janice M ^a   Lee, Lyndon ^a   Huang, Andrew J W ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

BIGH3; Keratoepithelin; Refolding

Indexed keywords

ESCHERICHIA COLI;

EID: 16544362414     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.12.020     Document Type: Article

References (27)

1. J. Skonier, M. Neubauer, L. Madisen, K. Bennett, G.D. Plowman, A.F. Purchio, cDNA cloning and sequence analysis of beta ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta, DNA Cell Biol. 11 (1992) 511-522.
2. K. Hashimoto, M. Noshiro, S. Ohno, T. Kawamoto, H. Satakeda, Y. Akagawa, K. Nakashima, A. Okimura, H. Ishida, T. Okamoto, H. Pan, M. Shen, W. Yan, Y. Kateo, Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/βig-h3) that binds to collagen, Biochim. Biochem. Acta 1355 (1997) 303-314.
3. I.M. Rawe, Q. Zhan, R. Burrows, K. Bunette, C. Cintron, Beta-ig3, Molecular cloning and in situ hybridization in corneal tissues, Invest. Ophthalmol. Vis. Sci. 38 (1997) 893-900.
4. T. Kawamoto, M. Noshiro, M. Shen, K. Nakamasu, K. Hashimoto, Y. Kawashima-Ohya, Y. Kateo, Structural and phylogenetic analyses of RGD-CAP/βig-h3, a fasciclin-like adhesion protein expressed in chick chondrocytes, Biochim. Biochem. Acta 1395 (1998) 288-292.
5. M.A. Gibson, J.S. Kumaratilake, E.G. Cleary, Immunohistochemical and ultrastructural localization of MP78/70 (/βig-h3) in extracellular matrix of developing and mature bovine tissues, J. Histochem. Cytochem. 45 (1997) 1683-1696.
6. K. Hirano, G.K. Klintworth, Q. Zhan, K. Bennett, C. Cintron, Beta ig-h3 is synthesized by corneal epithelium and perhaps endothelium in Fuchs' dystrophic corneas, Curr. Eye. Res. 15 (1996) 965-972.
7. J.E. Kim, R.W. Park, J.Y. Choi, Y.C. Bae, K.S. Kim, C.K. Joo, I.S. Kim, Molecular properties of wild-type and mutant βIG-H3 proteins, Invest. Ophthalmol. Vis. Sci. 43 (2002) 656-661.
8. J.E. Kim, E.H. Kim, E.H. Han, R.W. Park, I.H. Park, S.H. Jun, J.C. Kim, M.F. Young, I.S. Kim, A TGFβ-inducible cell adhesion molecule, βig-h3, is downregulated in melorheostosis and involved in osteogenesis, J. Cell. Biochem. 77 (2000) 169-178.
9. 1, Biochim. Biochem. Acta 1451 (1999) 196-205.
10. R.G. LeBaron, K.I. Bezverkov, M.P. Zimber, R. Pavelec, J. Skonier, A.F. Purchio, βIG-H3, a novel secretory protein inducible by transforming growth factor-β, is present in normal skin and promotes the adhesion and spreading of dermal fibroblasts in vitro, J. Invest. Dermatol. 104 (1995) 844-849.
11. J.E. Kim, S.J. Kim, B.H. Lee, R.W. Park, K.S. Kim, I.S. Kim, Identification of motifs for cell adhesion within the repeated domains of transforming growth factor-β-induced gene, βig-h3, J. Biol. Chem. 275 (2000) 30907-30915.
12. J.A. Ebel, K. Kuhn, in: J.A. Ebel (Ed.), Molecular Biology Intelligence Unit: Integrin-ligand interaction, Springer-Verlag, Heidelberg Germany, 1997, pp. 1-40.
13. 1 integrin, Biochem. Biophys. Res. Comm. 294 (2002) 940-948.
14. J. Skonier, K. Bennette, V. Rothwell, S. Kosowski, G. Plowman, P. Wallace, S. Edelhoff, C. Disteche, M. Neubauer, H. Marqardt, J. Rodgers, A.F. Purcio, βig-h3: a transforming growth factor-b-responsive gene, DNA Cell Biol. 13 (1994) 571-584.
15. S.C. Dieudonne, J.M. Kerr, T. Xu, B. Sommer, A.R. DeRubeis, S.A. Kuznetsov, I.S. Kim, P.G. Robey, M.F. Young, Differential display of human marrow stromal cells reveals unique mRNA expression patterns in response to dexamethasone, J. Mol. Biochem. 76 (1999) 231-243.
16. M. Wang, F. Munier, K. Araki-Saski, D. Schorderet, TGFBI gene transcript is transforming growth factor-beta1-responsive and cell density-dependent in a human corneal epithelial cell line, Ophthalmic Genet. 23 (2002) 237-245.
17. J. Escribano, N. Hernando, S. Ghosh, J. Crabb, M. Coca-Prados, cDNA from human ocular ciliary epithelium homologous to beta ig-h3 is preferentially expressed as an extracellular protein in the corneal epithelium, J. Cell Physiol. 160 (1994) 511-521.
18. F.L. Munier, E. Korvatska, A. Djemai, D. Le Paslier, L. Zografos, G. Pescia, D.F. Schorderet, Kerato-epithelin mutations in four 5q31-linked corneal dystrophies, Nat. Genet. 15 (1997) 247-251.
19. G.K. Klintworth, Advances in the molecular genetics of corneal dystrophies, Am. J. Ophthalmol. 128 (1999) 747-754.
20. F.L. Munier, B.E. Frueh, P. Othenin-Girard, S. Uffer, P. Cousin, M.X. Wang, E. Heon, G.C.M. Black, M.A. Blasi, E. Balestrazzi, B. Lorenz, R. Escoto, R. Barraquer, M. Hoeltzenbin, B. Gloor, M. Fossarello, A.D. Singh, Y. Arsenijevic, L. Zografos, D.F. Schorderet, BIGH3 mutation spectrum in corneal dystrophies, Invest. Ophthalmol. Vis. Sci. 43 (2002) 949-954.
21. 110-131 peptides, and its relevance for lattice corneal amyloid dystrophy type I, Biochem. Biophys. Res. Commun. 273 (2000) 649-653.
22. E. Korvatska, H. Henry, Y. Mashima, M. Yamada, C. Bachmann, F.L. Munier, D.F. Schoderet, Amyloid and non-amyloid forms of 5q31-linked corneal dystrophy resulting from kerato-epithelin mutations at Arg-124 are associated with abnormal turnover of the protein, J. Biol. Chem. 275 (2000) 11465-11469.
23. P.C. Billings, J.C. Whitbeck, C.S. Adams, W.R. Abrams, A.J. Cohen, B.N. Engelsberg, P.S. Howard, J. Rosebloom, The transforming growth factor-β-inducible matrix protein βig-h3 interacts wit fibronectin, J. Biol. Chem. 277 (2002) 28003-28009.
24. V.M. Borderie, N. Mourra, L. Laroche, Influence of fetal calf serum, fibroblast growth factors, and hepatocyte growth factor on three-dimensional cultures of human keratocytes in collagen gel matrix, Graefes Arch. Clin. Exp. Ophthalmol. 237 (1999) 861-869.
25. D. Arora, N. Khanna, Method for increasing the yield of properly folded recombinant y interferon from inclusion bodies, J. Biotechnol. 52 (1996) 127-133.
26. M. Suenaga, H. Ohmae, S. Tsuji, T. Itoh, O. Nishimura, Renaturation of recombinant human neurotrophin-3 from inclusion bodies using a suppressor agent of aggregation, Biotechnol. Appl. Biochem. 28 (1998) 119-124.
27. K. Tsumoto, D. Ejima, I. Kumagai, T. Arakawa, Practical considerations in refolding proteins from inclusion bodies, Protein Expr. Purif. 28 (2003) 1-8.