The NeuC Protein of Escherichia coli K1 is a UDP N-Acetylglucosamine 2-Epimerase

Journal of Bacteriology

Volumn 186, Issue 3, 2004, Pages 706-712

  Vann, Willie F ^b   Daines, Dayle A ^a,e   Murkin, Andrew S ^c   Tanner, Martin E ^c   Chaffin, Donald O ^d   Rubens, Craig E ^d   Vionnet, Justine ^b   Silver, Richard P ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^d UNIVERSITY OF WASHINGTON   (United States)

^e SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; INTEIN; MANNOSAMINE; PROTEIN NEUC; SIALIC ACID; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE 2 EPIMERASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL MENINGITIS; BACTERIAL VIRULENCE; BIOSYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ESCHERICHIA COLI; EUKARYOTE EVOLUTION; EXPRESSION VECTOR; GENE CLUSTER; GENE DELETION; GENETIC CODE; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PAPER CHROMATOGRAPHY; PHOSPHORUS NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTON NUCLEAR MAGNETIC RESONANCE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STREPTOCOCCUS AGALACTIAE;

CARBOHYDRATE EPIMERASES; ESCHERICHIA COLI PROTEINS; MAGNETIC RESONANCE SPECTROSCOPY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA; PROKARYOTA; STREPTOCOCCUS; STREPTOCOCCUS AGALACTIAE; VECTORS;

EID: 1642581499     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.3.706-712.2004     Document Type: Article

References (43)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Annunziato, P. W., L. F. Wright, W. F. Vann, and R. P. Silver. 1995. Nucleotide sequence and genetic analysis of the neuD and neuB genes in region 2 of the polysialic acid gene cluster of Escherichia coli K1. J. Bacteriol. 177:312-319.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1988. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
4. Bliss, J. M., and R. P. Silver. 1996. Coating the surface: a model for expression of capsular polysialic acid in Escherichia coli K1. Mol. Microbiol. 21:221-231.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. Chaffin, D. O., and C. E. Rubens. 1998. Blue/white screening of recombinant plasmids in gram-positive bacteria by interruption of alkaline phosphatase gene (phoZ) expression. Gene 219:91-99.
7. Chou, W. K., S. Hinderlich, W. Reutter, and M. E. Tanner. 2003. Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase. J. Am. Chem. Soc. 125:2455-2461.
8. Cross, A. S. 1990. The biologic significance of bacterial encapsulation. Curr. Top. Microbiol. Immunol. 150:87-95.
9. Daines, D. A., L. F. Wright, D. O. Chaffin, C. E. Rubens, and R. P. Silver, 2000. NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1. FEMS Microbiol. Lett. 189:281-284.
10. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
11. Donnenberg, M. S., and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59:4310-4317.
12. Effertz, K., S. Hinderlich, and W. Reutter. 1999. Selective loss of either the epimerase or kinase activity of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase due to site-directed mutagenesis based on sequence alignments. J. Biol. Chem. 274:28771-28778.
13. Haft, R. F., M. R. Wessels, M. F. Mebane, N. Conaty, and C. E. Rubens. 1996. Characterization of cpsF and its product CMP-N-acetylneuraminic acid synthetase, a group 13 streptococcal enzyme that can function in K1 capsular polysaccharide biosynthesis in Escherichia coli. Mol. Microbiol. 19:555-563.
14. Hinderlich, S., R. Stasche, R. Zeitler, and W. Reutter. 1997. A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis in rat liver. J. Biol. Chem. 272:24313-24318.
15. Kasper, D. L., J. L. Winkelhake, D. W. Zollinger, B. L. Brandt, and M. S. Artenstein. 1973. Immunochemical similarity between polysaccharide antigens of Escherichia coli O7:KIL:NM and group B Neisseria meningitidis. J. Immunol. 110:262-268.
16. Keenleyside, W. J., and C. Whitfield. 1996. A novel pathway for O-polysaccharide biosynthesis in Salmonella enterica serovar Borreze. J. Biol. Chem. 271:28581-28592.
17. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
18. Lai, E. C. K., and S. G. Withers. 1994. Stereochemistry and kinetics of the hydration of 2-acetamido-D-glucal by β-N-acetylhexosaminidases. Biochemistry 33:14743-14749.
19. Logan, S. M., J. F. Kelly, P. Thibault, C. P. Ewing, and P. Guerry. 2002. Structural heterogeneity of carbohydrate modifications affects serospecificity of Campylobacter flagellins. Mol. Microbiol. 46:587-597.
20. Morgan, P. M., R. F. Sala, and M. E. Tanner. 1997. Eliminations catalyzed by UDP-N-acetylglucosamine 2-epimerase. J. Am. Chem. Soc. 119:10269-10277.
21. Moxon, E. R., and J. S. Kroll. 1990. The role of bacterial polysaccharide capsules as virulence factors. Curr. Top. Microbiol. Immunol. 150:65-85.
22. Petersen, M., Fessner, W.-D., M. Frosch, and E. Luneberg. 2000. The siaA gene involved in capsule polysaccharide biosynthesis of Neisseria meningitidis B codes for N-acylglucosamine-6-phosphate 2-epimerase activity. FEMS Microbiol. Lett. 184:161-164.
23. Pravdic, N., I. Franjic-Mihalic, and B. Danilov. 1975. An improved synthesis of the 2-acetamido-D-glucal derivative 3,4,6-tri-O-acetyl-2-(N-acetylacetamido)-1, 5-anhydro-2-deoxy-D-arabino-hex-1-enitol. Carbohydr. Res. 45:302-306.
24. Rick, P. D., and R. P. Silver. 1996. Enterobacterial common antigens and capsular polysaccharides, p. 104-122. In F. C. Neidhardt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. American Society for Microbiology, Washington, D.C.
25. Ringenberg, M. A., S. M. Steenbergen, and E. R. Vimr. 2003. The first committed step in the biosynthesis of sialic acid by Escherichia coli K1 does not involve a phosphorylated N-acetylmannosamine intermediate. Mol. Microbiol. 50:961-975.
26. Robbins, J. B., George, J. H. McCracken, E. C. Gotschlich, F. Orskov, I. Orskov, and L. A. Hanson. 1974. Escherichia coli K1 capsular polysaccharide associated with neonatal meningitis. N. Engl. J. Med. 290:1216-1220.
27. Samuel, J., and M. E. Tanner. 2002. Mechanistic aspects of carbohydrate epimerization. Nat. Prod. Rep. 19:261-277.
28. Silver, R. P., and E. R. Vimr. 1990. Polysialic acid capsule of Escherichia coli K1, p. 39-60. In V. L. C. B. H. Iglewski (ed.), Molecular basis of microbial pathogenesis. Academic Press, Inc., San Diego, Calif.
29. Silver, R. P., W. F. Vann, and W. Aaronson. 1984. Genetic and molecular analyses of Escherichia coli K1 antigen genes. J. Bacteriol. 157:568-575.
30. Sommar, K. M., and D. B. Ellis. 1972. Uridine diphosphate N-acetyl-D-glucosamine-2-epimerase from rat liver. II. Studies on the mechanism of action. Biochim. Biophys. Acta 268:590-595.
31. Steenbergen, S. M., T. J. Wrona, and E. R. Vimr. 1992. Functional analysis of the sialyltransferase complexes in Escherichia coli K1 and K92. J. Bacteriol. 174:1099-1108.
32. Sussman, M. 1997. Escherichia coli and human disease, p. 3-48. In M. Sussman (ed.), Escherichia coli: mechanisms of virulence. Cambridge University Press, Cambridge, England.
33. Troy, F. A. 1992. Polysialylation: from bacteria to brains. Glycobiology 2:5-23.
34. Unhanand, M., M. M. Mustafa, J. G. H. McCracken, and J. D. Nelsen. 1993. Gram-negative enteric bacillary meningitis: a twenty-one-year experience. J. Pediatr. 122:15-21.
35. Vann, W. F., R. P. Silver, C. Abeijon, K. Chang, W. Aaronson, A. Sutton, C. W. Finn, W. Lindner, and M. Kotsatos. 1987. Purification, properties, and genetic location of Escherichia coli cytidine 5′-monophosphate N-acetylneuraminic acid synthetase. J. Biol. Chem. 262:17556-17562.
36. Vann, W. F., J. J. Tavarez, J. Crowley, E. Vimr, and R. P. Silver. 1997. Purification and characterization of the Escherichia coli K1 neuB gene product N-acetylneuraminic acid synthetase. Glycobiology 7:697-701.
37. Vimr, E., Steenbergen, S., and M. Cieslewicz. 1995. Biosynthesis of the polysialic acid capsule in Escherichia coli K1. J. Ind. Microbiol. 15:352-360.
38. Vimr, E. R., and F. A. Troy. 1985. Identification of an inducible catabolic system for sialic acids (nan) in Escherichia coli. J. Bacteriol. 164:845-853.
39. Vimr, E. R., R. D. McCoy, H. F. Vollger, N. C. Wilkison, and F. A. Troy. 1984. Use of prokaryotic-derived probes to identify poly(sialic acid) in neonatal neural membranes. Proc. Natl. Acad. Sci. USA 81:1971-1975.
40. Whitfield, C., and I. S. Roberts. 1999. Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol. Microbiol. 31:1307-1319.
41. Zapata, G., J. M. Crowley, and W. F. Vann. 1992. Sequence and expression of the Escherichia coli K1 neuC gene product. J. Bacteriol. 174:315-319.
42. Zapata, G., W. F. Vann, W. Aaronson, M. S. Lewis, and M. Moos. 1989. Sequence of the cloned Escherichia coli K1 CMP-N-acetylneuraminic acid synthetase gene. J. Biol. Chem. 264:14769-14774.
43. Zeitler, R., B. J., C. Bauer, and W. Reutter. 1992. Inhibition of the biosynthesis of N-acetylneuraminic acid by metal ions and selenium in vitro. Biometals 5:103-109.