Quantification of 5,6-Dihydrouracil by HPLC-Electrospray Tandem Mass Spectrometry

Clinical Chemistry

Volumn 50, Issue 1, 2004, Pages 236-238

  Van Kuilenburg, André B P ^a,b   Van Lenthe, Henk ^a   Van Cruchten, Arno ^a   Kulik, Willem ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROURACIL;

ARTICLE; ELECTROSPRAY; HEMOCONCENTRATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MONITORING; PLASMA; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; REPRODUCIBILITY; SCREENING; SIGNAL NOISE RATIO; TANDEM MASS SPECTROMETRY; TIME; URINALYSIS; URINE LEVEL;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; INFANT, NEWBORN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; URACIL; URIDINE;

EID: 1642533451     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2003.026229     Document Type: Article

References (5)

1. van Lenthe H, van Kuilenburg ABP, Ito T, Bootsma AH, van Cruchten A, Wada Y, et al. Defects in pyrimidine degradation identified by HPLC-electrospray tandem mass spectrometry of urine specimens or urine-soaked filter paper strips. Clin Chem 2000;46:1916-22.
2. Gamelin E, Boisdron-Celle M, Guérin-Meyer V, Delva R, Lortholary A, Genevieve F, et al. Correlation between uracil and dihydrouracil plasma ratio, fluorouracil (5-FU) pharmacokinetic parameters, and tolerance in patients with advanced colorectal cancer: a potential interest for predicting 5-FU toxicity and determining optimal 5-FU dosage. J Clin Oncol 1999;17:1105-10.
3. Garg MB, Sevester JC, Sakoff JA, Ackland SP. Simple liquid chromatographic method for the determination of uracil and dihydrouracil plasma levels: a potential pretreatment predictor of 5-fluorouracil toxicity. J Chromatogr B 2002;774:223-30.
4. Sumi S, Imaeda M, Kidouchi K, Ohba S, Hamajima N, Kodama K, et al. Population and family studies of dihydropyrimidinuria: prevalence, inheritance mode, and risk of fluorouracil toxicity. Am J Med Genet 1998;78:336-40.
5. Topp H, Duden R, Schöch G. 5,6-Dihydrouridine: a marker ribonucleoside for determining whole body degradation rates of transfer RNA in man and rats. Clin Chim Acta 1993;218:73-82.