Development of an in situ hybridisation procedure for the detection of sole aquabirnavirus in infected fish cell cultures

Journal of Virological Methods

Volumn 116, Issue 2, 2004, Pages 133-138

  Alonso, M C ^a   Cano, I ^a   Castro, D ^a   Perez Prieto, S I ^b   Borrego, J J ^a  

^a UNIVERSITY OF MÁLAGA   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

Immunofluorescence; In situ hybridisation; Sole aquabirnavirus; Tissue culture

Indexed keywords

COMPLEMENTARY DNA; VIRUS RNA;

AQUABIRNAVIRUS; ARTICLE; CELL CULTURE; CELL LABELING; CYTOPLASM; DNA PROBE; EMBRYO CELL; FIBROBLAST; FISH; IMMUNOFLUORESCENCE; IN SITU HYBRIDIZATION; INFECTIOUS PANCREATIC NECROSIS VIRUS; INOCULATION; NONHUMAN; ONCORHYNCHUS TSHAWYTSCHA; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEROTYPE; VIRUS DETECTION; VIRUS INFECTION;

AQUABIRNAVIRUS; INFECTIOUS PANCREATIC NECROSIS VIRUS; LEPOMIS MACROCHIRUS; ONCORHYNCHUS TSHAWYTSCHA;

EID: 1642518240     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2003.11.003     Document Type: Article

References (22)

1. 35S -labelled probe J. Virol. Meth. 25:1989;181-190.
2. Allan G.M., Smyth J.A., Todd D., McNutly M.S. In-situ hybridization for the detection of chicken anaemia virus in formalin-fixed, paraffin embedded sections. Avian Dis. 37:1993;177-182.
3. Amos, K.H., 1985. Procedures for the Detection and Identification of Certain Fish Pathogens, third ed. American Fisheries Society, Corvallis.
4. Babin M., Hernandez C., Sanchez C., Dominguez J. Immunodot assay for detection of IPNV virus in organ homogenates. Bull. Eur. Ass. Fish Pathol. 11:1991;65-67.
5. Biering E., Bergh O. Experimental infection of Atlantic halibut Hippoglossus hipoglossus L., yolk-sac larvae with infectious pancreatic necrosis virus: detection of virus by immunohistochemistry and in situ hybridization. J. Fish Dis. 19:1996;405-413.
6. Blake S., Lee M.K., Singer J., McAllister P., Nicholson B.L. Detection and identification of aquatic birnaviruses by polymerase chain reaction assay. J. Clin. Microbiol. 33:1995;835-839.
7. Bruce L.D., Redman R.M., Lightner D.V. Application of gene probes to determine target organs of a penaeid shrimp baculovirus using in situ hybridization. Aquaculture. 120:1994;45-51.
8. Dopazo C.P., Hetrick F.M., Samal S.K. Used of cloned cDNA probes for diagnosis of infectious pancreatic necrosis virus infection. J. Fish Dis. 17:1994;1-16.
9. Evensen O., Rimstad E. Immunohistochemical identification of infectious pancreatic necrosis virus in paraffin-embedded tissues of Atlantic salmon (Salmo salar). J. Vet. Diagn. Invest. 2:1990;288-293.
10. Garcia-Rosado E., Castro D., Cano I., Perez-Prieto S.I., Borrego J.J. Serological techniques for detection of lymphocystis virus in fish. Aquat. Living Resour. 15:2002;179-185.
11. Gregory A. Detection of infectious salmon anaemia virus (ISAV) by in situ hybridisation. Dis. Aquat. Org. 50:2002;105-110.
12. Kim O. Development of in situ Nest PCR and comparison of five molecular biological diagnostic methods for the detection of intracellular viral DNAs in paraffin sections. J. Vet. Med. Sci. 65:2003;231-235.
13. Kim O., Chae C. Comparison of reverse transcription polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine epidemic diarrhea virus in pigs. Can. J. Vet. Res. 66:2002;112-116.
14. Lorezen E., Carstensen B., Olensen N.J. Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Dis. Aquat. Org. 37:1999;81-88.
15. Nuovo, G.J., 1994. PCR in situ Hybridization. Protocols and Applications, second ed. Raven Press, New York.
16. Perez-Prieto S., Garcia-Rosado E., Rodriguez S., Castro D., Borrego J.J. Antigenic properties and experimental transmission to several fish species of a marine birnavirus isolated from sole (Solea senegalensis). Vet. Microbiol. 82:2001;11-25.
17. Rimstad E., Krona R., Hornes E., Olsvik O., Hylleseth B. Detection of infectious pancreatic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe. Vet. Microbiol. 23:1990;211-219.
18. Rodriguez S., Vilas P., Sarasquete M.C., Rodriguez R.B., Gutierrez M.C., Perez-Prieto S. Isolation and preliminary characterization of a birnavirus from Solea senegalensis in Southwest Spain. J. Aquat. Anim. Health. 9:1997;295-300.
19. Rodriguez S., Borrego J.J., Perez-Prieto S.I. Comparative evaluation of five serological methods and RT-PCR assay for the detection of IPNV in fish. J. Virol. Meth. 97:2001;23-31.
20. Rodriguez-Saint Jean, S., Borrego, J.J., Perez-Prieto, S.I., in press. Infectious pancreatic necrosis virus: biology, pathogenesis and diagnostic methods. Adv. Virus Res.
21. Sano N., Moriwake M., Hondo R., Sano T. Herpesvirus cyprini: a search for viral genome in infected fish by in situ hybridization. Vet. Microbiol. 23:1993;211-219.
22. Yang H., Wanner J.B., Roper S.D., Chaudhari N. An optimized method for in situ hybridization with signal amplification that allows the detection of rare mRNAs. J. Histochem. Cytochem. 47:1999;431-446.