A multiplex PCR assay for the identification of animal species in feedstuffs

Molecular and Cellular Probes

Volumn 18, Issue 2, 2004, Pages 81-87

  Dalmasso, A ^a   Fontanella, E ^b   Piatti, P ^b   Civera, T ^a   Rosati, S ^a   Bottero, M T ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b Laboratorio Chimico Camera Commercio   (Italy)

Author keywords

Feedstuff; Meat and bone meal; Multiplex polymerase chain reaction; Species identification

Indexed keywords

MITOCHONDRIAL DNA; PRIMER DNA;

ANALYTIC METHOD; ANIMAL FOOD; ANIMAL TISSUE; ARTICLE; BOVIDS; CONTROLLED STUDY; DATA BASE; EXPERIMENTAL DESIGN; FISH; FOOD ANALYSIS; FRAGMENTATION REACTION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT; POLYMERASE CHAIN REACTION; POULTRY; PRIORITY JOURNAL; SPECIES COMPARISON; VALIDATION PROCESS;

ANIMAL FEED; ANIMALS; DNA, MITOCHONDRIAL; FISHES; POLYMERASE CHAIN REACTION; POULTRY; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 16S; RNA, TRANSFER, VAL; RUMINANTS; SWINE;

ANIMALIA; BOVINAE;

EID: 1642420995     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2003.09.006     Document Type: Article

References (14)

1. European Commission. Commission decision of 27 March 2002 amending council decision 2000/766/EC and commission decision 2001/9/EC with regard to transmissible spongiform encephalopathies and the feeding of animal proteins. (2002/248/EC). Official J Eur Communities. L84:(28/3/2002):2002;71-72.
2. Momcilovic D., Rasooly A. Detection and analysis of animal materials in food and feed. J Food Prot. 63:2000;1602-1609.
3. Meyer R., Candrian U. PCR-based DNA analysis for the identification and characterization of food components. Lebensmittel-Wissenhauf und-Technologie. 29:1996;1-9.
4. Krcmar P., Rencova E. Identification of bovine-specific DNA in feedstuffs. J Food Prot. 64:2001;117-119.
5. Myers M.J., Friedman S.L., Farrell D.E., Dove-Pettit D.A., Bucker M.F., Kelly S., Madzo S., Campbell W., Wang R.F., Daine D., Cerniglia C.E. Validation of a polymerase chain reaction method for the detection of rendered bovine-derived materials in feedstuffs. J Food Prot. 64:2001;564-566.
6. Tartaglia M., Saulle E., Pestalozza S., Morelli L., Antonucci G., Battaglia P.A. Detection of bovine mitochondrial DNA in ruminant feeds: a molecular approach to test for the presence of bovine-derived materials. J Food Prot. 61:1998;513-518.
7. Wang R.-F., Myers M.J., Campbell W., Cao W.-W., Paine D., Cerniglia C.E. A rapid method for PCR detection of bovine materials in animal feedstuffs. Mol Cell Probes. 14:2000;1-5.
8. Lahiff S., Glennon M., O'Brien L., Lyng J., Smith T., Maher M., Shilton N. Species-specific PCR for the identification of ovine, porcine and chicken species in meat and bone meal (MBM). Mol Cell Probes. 15:2001;27-35.
9. Myers M.J., Yancy H.F., Farrell D.E. Research note: characterization of a polymerase chain reaction-based approach for the simultaneous detection of multiple animal-derived materials in animal feed. J Food Prot. 66:2003;1085-1089.
10. Bottero M.T., Dalmasso A., Nucera D., Turi R.M., Rosati S., Squadrone S., Goria M., Civera T. Development of a PCR assay for the detection of animal tissues in ruminant feeds. J Food Prot. 2003;66. in press.
11. Higgins D.G., Bleasby A.J., Fuchs R. CLUSTAL W: improved software for multiple sequence alignment. Comput Appl Biosci. 8:1992;4420-4449.
12. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J Mol Biol. 215:1990;403-410.
13. European Commission. Commission directive of 13 November 1998 establishing guidelines for the microscopic identification and estimation of constituents of animal origin for the official control of feedingstuffs. (98/88/EC). Official J Eur Communities. L318:(27/11/1998):1998;45-50.
14. Lahiff S., Glennon M., Lyng J., Smith T., Shilton N., Maher M. Real-time polymerase chain reaction detection of bovine DNA in meat and bone meal samples. J Food Prot. 65:2002;1158-1165.