Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b

European Journal of Biochemistry

Volumn 271, Issue 6, 2004, Pages 1209-1218

  Zara, Vincenzo ^a   Palmisano, Ilaria ^a   Conte, Laura ^a   Trumpower, Bernard L ^b  

^a UNIVERSITY OF SALENTO   (Italy)

^b DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

Assembly; Cytochrome bc1; Saccharomyces cerevisiae; Supernumerary subunits

Indexed keywords

CYTOCHROME B; CYTOCHROME C1; UBIQUINOL CYTOCHROME C REDUCTASE;

ARTICLE; COMPLEX FORMATION; DELETION MUTANT; ENZYME SUBUNIT; MITOCHONDRIAL MEMBRANE; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SACCHAROMYCES CEREVISIAE;

BLOTTING, WESTERN; CYTOCHROMES B; DIMERIZATION; ELECTRON TRANSPORT COMPLEX III; GENE DELETION; GENES, FUNGAL; INTRACELLULAR MEMBRANES; MITOCHONDRIA; MODELS, MOLECULAR; PHENOTYPE; PROTEIN SUBUNITS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE DELETION;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 1642404614     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04024.x     Document Type: Article

References (40)

1. 1 complexes of microorganisms. Microbiol. Rev. 54, 101-129.
2. Berry, E.A., Guergova-Kuras, M., Huang, L.S. & Crofts, A.R. (2000) Structure and function of cytochrome bc complexes. Annu. Rev. Biochem. 69, 1005-1075.
3. 1 complex of bovine heart mitochondria. Science 277, 60-66.
4. 1. Nature 392, 677-684.
5. 1 complex. Science 281, 64-71.
6. 1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody fragment. Structure 8, 669-684.
7. 1 complex with its bound substrate cytochrome c. Proc. Natl Acad. Sci. USA 99, 2800-2805.
8. 1 complex. J. Biol. Chem. 269, 12947-12953.
9. Tzagoloff, A. (1995) Ubiquinol-cytochrome-c oxidoreductase from Saccharomyces cerevisiae. Methods Enzymol. 260, 51-63.
10. Tzagoloff, A., Wu, M. & Crivellone, M. (1986) Assembly of the mitochondrial membrane system. Characterization of COR1, the structural gene for the 44-kilodalton core protein of yeast coenzyme QH2-cytochrome c reductase. J. Biol. Chem. 261, 17163-17169.
11. Oudshoorn, P., Van Steeg, H., Swinkels, B.W., Schoppink, P. & Grivell, L.A. (1987) Subunit II of yeast QH2: cytochrome-c oxidoreductase. Nucleotide sequence of the gene and features of the protein. Eur. J. Biochem. 163, 97-103.
12. Van Loon, A.P., De Groot, R.J., Haan, M., Dekker, A. & Grivell, L.A. (1984) The DNA sequence of the nuclear gene coding for the 17-kd subunit VI of the yeast ubiquinol-cytochrome c reductase: a protein with an extremely high content of acidic amino acids. EMBO J. 3, 1039-1043.
13. De Haan, M., Van Loon, A.P.G.M., Kreike, J., Vaessen, R.T.M.J. & Grivell, L.A. (1984) The biosynthesis of the ubiquinolcytochrome c reductase complex in yeast. DNA sequence analysis of the nuclear gene coding for the 14 kDa subunit. Eur. J. Biochem. 138, 169-177.
14. Maarse, A.C. & Grivell, L.A. (1987) Nucleotide sequence of the gene encoding the 11-kDa subunit of the ubiquinol-cytochrome c oxidoreductase in S. cerevisiae. Eur. J. Biochem. 165, 419-425.
15. Phillips, J.D., Schmitt, M.E., Brown, T.A., Beckmann, J.D. & Trumpower, B.L. (1990) Isolation and characterization of QCR9, a nuclear gene encoding the 7.3-kDa subunit 9 of the Saccharomyces cerevisiae ubiquinol-cytochrome c oxidoreductase complex. J. Biol. Chem. 265, 20813-20821.
16. Yang, X. & Trumpower, B.L. (1988) Protonmotive Q cycle pathway of electron transfer and energy transduction in the three-subunit ubiquinol-cytochrome c oxidoreductase complex of Paracoccus denitrificans. J. Biol. Chem. 263, 11962-11970.
17. Pfanner, N. & Geissler, A. (2001) Versatility of the mitochondrial protein import machinery. Nat. Rev. Mol. Cell Biol. 2, 339-349.
18. Schoppink, P.J., Hemrika, W., Reynen, J.M., Grivell, L.A. & Berden, J.A. (1988) Yeast ubiquinol: cytochrome c oxidoreductase is still active after inactivation of the gene encoding the 17-kDa subunit VI. Eur. J. Biochem. 173, 115-122.
19. Maarse, A.C., De Haan, M., Schoppink, P.J., Berden, J.A. & Grivell, L.A. (1988) Inactivation of the gene encoding the 11-kDa subunit VIII of the ubiquinol-cytochrome-c oxidoreductase in Saccharomyces cerevisiae. Eur. J. Biochem. 172, 179-184.
20. Schoppink, P.J., Berden, J.A. & Grivell, L.A. (1989) Inactivation of the gene encoding the 14-kDa subunit VII of yeast ubiquinol cytochrome c oxidoreductase and analysis of the resulting mutant. Eur. J. Biochem. 181, 475-483.
21. Baudin, A., Ozier-Kalogeropoulos, O., Denouel, A., Lacroute, F. & Cullin, C. (1993) A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21, 3329-3330.
22. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168.
23. Guthrie, C. & Fink, G.R. (1991) Guide to Yeast Genetics and Molecular Biology. Academic Press, San Diego, CA.
24. 1 complex and can be suppressed by overexpression of subunit 9. J. Biol. Chem. 266, 14958-14963.
25. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
26. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
27. Dulley, J.R. & Grieve, P.A. (1975) A simple technique for eliminating interference by detergents in the Lowry method of protein determination. Anal. Biochem. 64, 136-141.
28. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
29. 1 complex is required for insertion of EPR-detectable iron-sulfur cluster into the Rieske iron-sulfur protein. J. Biol. Chem. 268, 11727-11736.
30. Berden, J.A., Schoppink, P.J. & Grivell, L.A. (1988) A model for the assembly of ubiquinol: cytochrome c oxidoreductase in yeast. In Molecular Basis of Biomembrane Transport (Palmieri, F. & Quagliariello, E., eds), pp. 195-208. Elsevier, Amsterdam.
31. Crivellone, M.D., Wu, M. & Tzagoloff, A. (1988) Assembly of the mitochondrial membrane system. J. Biol. Chem. 263, 14323-14333.
32. Rep, M. & Grivell, L.A. (1996) The role of protein degradation in mitochondrial function and biogenesis. Curr. Genet. 30, 367-380.
33. 1 complex is not essential for import into mitochondria in Saccharomyces cerevisiae but is essential for assembly of the complex. J. Biol. Chem. 272, 17495-17501.
34. 1 complex in S. cerevisiae may be involved in facilitating stability of the subcomplex with the Qcr8 protein and cytochrome b. Arch. Biochem. Biophys. 393, 215-221.
35. 1 and c. Equilibrium constants and kinetic probes. J. Biol. Chem. 262, 8103-8108.
36. 1, and causes temperature-sensitive petite growth in Saccharomyces cerevisiae. J. Biol. Chem. 269, 1270-1275.
37. 1 complex in Saccharomyces cerevisiae. J. Biol. Chem. 265, 17005-17011.
38. Gonzales-Halphen, D., Lindorfer, M.A. & Capaldi, R.A. (1988) Subunit arrangement in beef heart complex III. Biochemistry 27, 7021-7031.
39. 1 complex from beef heart. Methods Enzymol. 126, 224-237.
40. 1 complex. J. Biol. Chem. 277, 49397-49402.