An amino-acid substitution in the influenza-B NB protein affects ion-channel gating

Journal of Membrane Biology

Volumn 197, Issue 3, 2004, Pages 135-143

  Premkumar, A ^a   Ewart, G D ^a,b   Cox, G B ^a   Gage, P W ^a  

^a Australian National University   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

Amino acid substitution; Influenza B; Ion channel gating; NB protein; Transmembrane domain

Indexed keywords

ALANINE; CATION; CYSTEINE; ION CHANNEL; SERINE; THREONINE; UNCLASSIFIED DRUG; VIRUS NB PROTEIN; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; FLUORESCENCE; GENE MUTATION; HYDROPHOBICITY; INFLUENZA VIRUS B; ION TRANSPORT; LIPID BILAYER; MEMBRANE PERMEABILITY; NONHUMAN; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ION CHANNEL GATING; ION CHANNELS; KINETICS; LIPID BILAYERS; MEMBRANE POTENTIALS; MEMBRANES, ARTIFICIAL; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; VIRAL PROTEINS;

ANIMALIA; INFLUENZA B VIRUS; INFLUENZA VIRUS;

EID: 1642354367     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-004-0648-0     Document Type: Article

References (23)

1. Betakova, T., Nermut, M.V., Hay, A.J. 1996. The NB protein is an integral component of the membrane of influenza B virus. J. Gen. Virol. 77:2689-2694
2. Burmeister, W.P., Baudin, F., Cusack, S., Ruigrok, R.W. 1993. Comparison of structure and sequence of influenza B/Yamagata and B/Beijing neuraminidases shows a conserved "head" but much greater variability in the "stalk" and NB protein. Virology 192:683-686
3. Byerly, L., Suen, Y. 1989. Characterization of proton currents in neurones of the snail, Lymnaea stagnalis. J. Physiol. 413:75-89
4. Decoursey, T.E., Cherny, V.V. 1995. Voltage-activated proton currents in membrane patches of rat alveolar epithelial cells. J. Physiol. 489:299-307
5. Ewart, G.D., Sutherland, T., Gage, P.W., Cox, G.B. 1996. The vpu protein of human immunodeficiency virus type 1 forms cation-selective ion channels. J. Virol. 70:7108-7115
6. Fischer, W.B., Pitkeathly, M., Wallace, B.A., Forrest, L.R., Smith, G.R., Sansom M.S.P. 2000. Transmembrane peptide NB of influenza B: A simulatiom, structure and conductance study. Biochemistry 39:12708-12716
7. Fischer, W.B., Pitkeathly, M., Sansom M.S.P. 2001. Amantadin blocks channel activity of the transmembrane segment of the NB protein from influenza B. Eur. Biophys. J. 30:416-420
8. Galzi, J., Devillers-Thiery, A., Hussy, N., Bertrand, S., Changeux, J., Bertrand, D. 1992. Mutations in the channel domain of a neuronal nicotinic receptor convert ion selectivity from cationic to anionic. Nature 359:500-504
9. Guy, H.R., Conti F. 1990. Pursuing the structure and function of voltage-gated channels. Trends Neurosci. 6:201-206
10. Haddock, B.A., Downie, J.A. 1974. The reconstitution of functional respiratory chains in membranes from electron-transport-deficient mutant of Escherichia coli as demonstrated by quenching of atebrin fluorescence. Biochem. J. 142:703-706
11. Kerr, I.D., Sankararamakrishnan, R., Smart, O.S., Sansom, M.S.P. 1994. Parallel helix bundles and ion channels: molecular modeling via simulated annealing and restrained molecular dynamics. Biophys. J. 67:1501-1515
12. Lamb, R.A., Pinto, L.H. 1997. Do vpu and vpr of human immunodeficiency virus type1 and NB of influenza B virus have ion channel activities in the viral life cycles? Virology 229:1-11
13. Lear, J.D., Wasserman, Z.R., Degrado, W.F. 1988. Synthetic amphiphilic peptide models for protein ion channels [published erratum appears in Science 1989 Sep 29; 245(4925): 1437]. Science 240:1177-1181
14. Leonard, R.J., Labarca, C.G., Charnet, P., Davidson, N., Lester, H.A. 1988. Evidence that the M2 membrane-spanning region lines the ion channel pore of the nicotinic receptor. Science 242:1578-1581
15. Lu, Y.A., Clavijo, P., Galantino, M., Shen, Z.Y., Liu, W., Tam, J.P. 1991. Chemically unambiguous peptide immunogen: preparation, orientation and antigenicity of purified peptide conjugated to the multiple antigen peptide system. Mol. Immunol. 28:623-630
16. Miller, C. 1986. Ion channel reconstitution. Plenum Press, New York.
17. Sansom, M.S.P. 1992. An investigation of the role of serine and threonine sidechains in ion channel proteins. Eur. Biophys. J. 21:281-298
18. Sugrue, R.J., Hay, A.J. 1991. Structural characteristics of the M2 protein of influenza A viruses: evidence that it forms a tetrameric channel. Virology 180:617-624
19. Sunstrom, N.A., Premkumar, L.S., Premkumar, A., Ewart, G., Cox, G.B., Gage, P.W. 1996. Ion channels formed by NB, an influenza B virus protein. J. Membrane Biol 150:127-132
20. 2-terminal region. Mol. Cell Biol. 6: 4317-4328
21. Zebedee, S.L., Lamb, R.A. 1988. Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions. J. Virol. 62:2762-2772
22. Zhirnov, O.P. 1990. Solubilization of matrix protein M1/M from virions occurs at different pH for orthomyxo- and paramyxoviruses. Virology 176:274-279
23. Zhirnov, O.P. 1992. Isolation of matrix protein M1 from influenza viruses by acid-dependent extraction with nonionic detergent. Virology 186:324-330