Determination of accurate KI values for tight-binding enzyme inhibitors: An in silico study of experimental error and assay design

Analytical Biochemistry

Volumn 327, Issue 1, 2004, Pages 61-67

  Murphy, Dennis J ^a  

^a DREXEL UNIVERSITY   (United States)

Author keywords

Enzyme inhibition constant; Enzyme titration; Kinetics; Morrison equation; Nonlinear curve fitting; Structure activity relationship; Tight binding

Indexed keywords

CURVE FITTING; ENZYME KINETICS; ERRORS; NONLINEAR EQUATIONS; STRUCTURAL DESIGN;

INHIBITION CONSTANTS; MORRISON EQUATION; NONLINEAR CURVE FITTING; STRUCTURE ACTIVITY RELATIONSHIPS; TIGHT BINDING;

ENZYME INHIBITION;

ENZYME; ENZYME INHIBITOR;

ACCURACY; ARTICLE; BIOCHEMISTRY; COMPUTER MODEL; CONCENTRATION (PARAMETERS); DILUTION; ERROR; PRIORITY JOURNAL; SIMULATION; STRUCTURE ACTIVITY RELATION; VELOCITY; CHEMISTRY; COMPUTER SIMULATION; KINETICS; MATHEMATICAL COMPUTING; METABOLISM; PROTEIN BINDING; REPRODUCIBILITY; TECHNIQUE;

COMPUTER SIMULATION; ENZYME INHIBITORS; KINETICS; MATHEMATICAL COMPUTING; METHODS; PROTEIN BINDING; REPRODUCIBILITY OF RESULTS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 1642333892     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.12.018     Document Type: Article

References (9)

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