Biodiversity analysis of microbial community in the chem-bioflocculation treatment process

Biotechnology and Bioengineering

Volumn 89, Issue 6, 2005, Pages 656-659

  Xia, Siqing ^a   Wang, Feng ^a   Fu, Yigang ^a   Yang, Dianhai ^a   Ma, Xingmao ^b  

^a TONGJI UNIVERSITY   (China)

^b MISSOURI UNIVERSITY OF SCIENCE AND TECHNOLOGY   (United States)

Author keywords

Activated sludge; DGGE; Microbial community structures; PCR

Indexed keywords

ACTIVATED SLUDGE PROCESS; ELECTROPHORESIS; ENZYME KINETICS;

BIODIVERSITY ANALYSIS; DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE); MICROBIAL COMMUNITY STRUCTURES; POLYMERASE CHAIN REACTION (PCR);

MICROBIOLOGY;

DNA; RIBOSOME DNA;

ACTIVATED SLUDGE; ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; FLOCCULATION; MICROBIAL DIVERSITY; MICROBIAL GROWTH; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SPECIES IDENTIFICATION; WASTE WATER MANAGEMENT;

BACTERIA; BIODIVERSITY; DNA FINGERPRINTING; DNA, RIBOSOMAL; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENVIRONMENTAL MICROBIOLOGY; FLOCCULATION; POLYMERASE CHAIN REACTION; POPULATION DYNAMICS; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; WASTE DISPOSAL, FLUID;

EID: 16344394462     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20339     Document Type: Article

References (15)

1. Boon N, Eva MT, Willy V, Steven DS. 2003. Bioaugmentation as a tool to protect the structure and function of an activated-sludge microbial community against a 3-chloroaniline shock load. Appl Environ Microbiol 69(3):1511-1520.
2. Boon N, Marie C, Top EM, Verstraete W. 2000. Comparison of the spatial homogeneity of physico-chemical parameters and bacterial 16S rRNA genes in sediment samples from a dumping site for dredging sludge. Appl Microbiol Biotechnol 53:742-747.
3. Casserly C, Lenoardo E. 2003. Molecular monitoring of microbial diversity in an UASB reactor. Intl Biodeter Biodegr 52:7-12.
4. Hemant JP, Atya K, Aditi AM, Guppreet N. 2003. A novel approach for extraction of PCR-compatible DNA from activated sludge samples collected from different biological effluent treatment plants. J Microbiol Meth 52:315-323.
5. Muyzer G, Ellen CDW, Andre GU. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59(3):695-700.
6. Orlando C, Pinzani P, Pazzagli M. 1998. Developments in quantitative PCR. Clin Chem Lab Med 36:255-269.
7. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
8. Snaedr J, Rudolf A, Ingred H, Wolfgang L, Karl HS. 1997. Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl Environ Microbiol 63(7):2884-2896.
9. Timothy ML, Cindy HN, Lisa P, Alleman JE. 2000. Phylogenetic analysis of bacterial communities in mesophilic and thermophilic bioreactors treating pharmaceutical wastewater. Appl Environ Microbiol 66(9): 3951-3959.
10. Timothy ML, Cindy HN, Lisa P, Alleman JE. 2001. Aerobic biological treatment of a pharmaceutical wastewater: Effect of temperature on cod removal and bacterial community development. Wat Res 35(18): 4417-4425.
11. Vogelaar JCT, van Lier JB, Klapwijk B, Vries MC de, Lettinga G. 2002. Assessment of effluent turbidity in mesophilic and thermophilic activated sludge reactors-origin of effluent colloidal material. Appl Microbiol Biotechnol 59:105-111.
12. Wagner M, Amann R, Hilde L, Karl HS. 1993. Probing activated sludge with oligonucleotides specific for proteobacteria: Inadequacy of culture-dependent methods for describing microbial community structure. Appl Environ Microbiol 59(5): 1520-1525.
13. Watanabe K, Teramoto M, Futamata H. 1998. Molecular detection, isolation, and physiological characterization of functionally dominant phenol degrading bacteria in activated sludge. Appl Environ Microbiol 64(11):4396-4402.
14. Xia SQ, Yang DH, Gao TY. 2002. Experimental study on chemical and biological flocculation process for treating municipal sewage. Shanghai Environmental Sciences 22:16-18.
15. Zhou JZ, Mary AB, James MT. 1996. DNA recovery from soils of diverse composition. Appl Environ Microbiol 62:316-322.