Acute clinical, hematologic, serologic, and polymerase chain reaction findings in horses experimentally infected with a European strain of anaplasma phagocytophilum

Journal of Veterinary Internal Medicine

Volumn 19, Issue 2, 2005, Pages 232-239

  Franzen P ^a   Aspan, A ^b   Egenvall, A ^a   Gunnarsson, A ^b   Aberg L ^c   Pringle, J ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b NATIONAL VETERINARY INSTITUTE   (Sweden)

^c Djursjukhuset Bagarmossen   (Sweden)

Author keywords

Ehrlichia equi; Equine granulocytic ehrlichiosis; Human granulocytic ehrlichiosis; Sudden death

Indexed keywords

ANAPLASMA PHAGOCYTOPHILUM; ARTICLE; BACTERIAL INFECTION; BACTERIAL STRAIN; BLOOD SMEAR; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; EHRLICHIOSIS; GENETIC DIFFERENCE; HEMATOLOGY; HORSE; NEUTROPHIL; NONHUMAN; POLYMERASE CHAIN REACTION; SEROLOGY;

ANAPLASMA PHAGOCYTOPHILUM; ANIMALS; ANTIBODIES, BACTERIAL; BLOOD CELL COUNT; BODY TEMPERATURE; EHRLICHIOSIS; HORSE DISEASES; HORSES; POLYMERASE CHAIN REACTION;

ANAPLASMA; ANAPLASMA PHAGOCYTOPHILUM; EHRLICHIA; EQUIDAE; EQUUS CABALLUS;

EID: 16344393701     PISSN: 08916640     EISSN: None     Source Type: Journal    
DOI: 10.1892/0891-6640(2005)19<232:ACHSAP>2.0.CO;2     Document Type: Article

References (37)

1. Dumler JS, Barbet AF, Bekker CP, et al. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 2001;51:2145-2165.
2. Pusterla N, Lutz H, Braun U. Experimental infection of four horses with Ehrlichia phagocytophila. Vet Rec 1998;143:303-305.
3. Pusterla N, Pusterla JB, Braun U, Lutz H. Experimental cross-infections with Ehrlichia phagocytophila and human granulocytic Ehrlichia-like agent in cows and horses. Vet Rec 1999;145:311-314.
4. Stuen S, Artursson K, Olsson Engvall E. Experimental infection in lambs with an equine granulocytic ehrlichia species resembling the agent that causes human granulocytic ehrlichiosis (HGE). Acta Vet Scand 1998;39:491-497.
5. Pusterla N, Aandersson RJ, House JK, et al. Susceptibility of cattle to infection with Ehrlichia equi and the agent of human granulocytic ehrlichiosis. J Am Vet Med Assoc 2001;218:1160-1162.
6. Gribble DH. Equine ehrlichiosis. J Am Vet Med Assoc 1969;155:462-469.
7. Mesquita Neto F de, Ajauro T, Vieira RM, Gouveia AMG. Ehrlichia equi in horses from the municipality of Betim (Brazil). Arq Bras Med Vet Zootec 1984;36:203-207.
8. Bücher G, Grandras R, Apel G, Friedhoff KT. Der erste fall von ehrlichiosis beim pferd in Deutchland. Dtsch Tierärtztl Wochenschr 1984;91:408-409.
9. Hermann M, Bauman D, Lutz H, Wild P. Erster diagnostizierter fall von equiner Ehrlichiose in der Schweiz. Pferdeheilkunde 1985;1:247-250.
10. Bjöersdorff A, Johnssson A, Sjöström A-C, Madigan J. Ehrlichia equi-infektion diagnostiserad hos häst. Svensk Veterinärtidning 1990;42:357-360.
11. Korbutiak E, Schneiders D. Equine granulocytic ehrlichiosis in the UK. Vet Rec 1994;135:387-388.
12. Madigan JE. Equine ehrlichiosis. Vet Clin North Am Equine Pract 1993;9:423-428.
13. Reubel GH, Kimsey RB, Barlough JE, Madigan JE. Experimental transmission of Ehrlichia equi to horses through naturally infected ticks (Ixodes pacifinus) from northern California. J Clin Microbiol 1998;36:2131-2134.
14. Egenvall A, Franzen P, Gunnarsson A, et al. Cross-sectional study of the seroprevalence to Borrelia burgdorferi sensu lato and granulocytic Ehrlichia spp. and demographic, clinical and tick-exposure factors in Swedish horses. Prev Vet Med 2001;49:191-208.
15. Nyindo MBA, Ristic M, Lewis GE, et al. Immune response of ponies to experimental infection with Ehrlichia equi. Am J Vet Res 1978;39:15-18.
16. Corstvet RE, Gaunt SD, Karns PA, et al. Detection of humoral antigen and antibody by enzyme-linked immunosorbent assay in horses with experimentally induced Ehrlichia equi infection. J Diagn Invest 1993;5:37-39.
17. Barlough JE, Madigan JE, DeRock E, Bigornia L. Nested polymerase chain reaction for detection of Ehrlichia equi genomic DANN in horses and ticks (Ixodes pacificus). Vet Parasitol 1996;63:319-329.
18. Franzén P, Artursson, K, Gunnarsson A, et al. Experimentally induced equine granulocytic ehrlichiosis with a Swedish Ehrlichia sp. isolate. VIII International Conference on Equine Infectious Diseases, Dubai, 1998.
19. Madigan JE, Richter PJ, Kimsey RB, et al. Transmission and passage in horses of the agent of human granulocytic ehrlichiosis. J Infect Dis 1995;172:1141-1144.
20. Barlough JE, Madigan JE, DeRock E, et al. Protection against Ehrlichia equi is conferred by prior infection with the human granulocytic ehrlichia (HGE agent). J Clin Microbiol 1995;33:3333-3334.
21. Chang Y-F, Novocel V, Dubovi E, et al. Experimental infection of the human granulocytic ehrlichiosis agent in horses. Vet Parasitol 1998;78:137-145.
22. Pusterla N, Leutenegger CM, Chae JS, et al. Quantitative evaluation of ehrlichial burden in horses after experimental transmission of human granulocytic Ehrlichia agent by intravenous inoculation with infected leukocytes and by infected ticks. J Clin Microbiol 1999;37:4042-4044.
23. Pusterla N, Madigan JE, Asanovich KM, et al. Experimental inoculation with human granulocytic Ehrlichia agent derived from high- and low-passage cell culture in horses. J Clin Microbiol 2000;38:1276-1278.
24. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697-703.
25. Drancourt M, Bollet C, Raoult D. Stenotrophomonas africana sp. nov., an opportunistic human pathogen in Africa. Int J Syst Bacteriol 1997;47:160-163.
26. Bjöersdorff A, Bagert B, Massung RF, et al. Isolation and characterization of two European strains of Ehrlichia phagocytophila of equine origin. Clin Diagn Lab Immunol 2002;9:341-343.
27. Chae J, Foley JE, Dumler JS, Madigan JE. Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from northern California. J Clin Microbiol 2000;38:1364-1369.
28. Johansson KE, Pettersson P, Uhlen M, et al. Identification of the causative agent of granulocytic ehrlichiosis in Swedish dogs and horses by direct solid phase sequencing of PCR products from the 16S rRNA gene. Res Vet Sci 1995;58:1109-112.
29. Olsson Engvall E, Pettersson B, Persson M, et al. A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses and cattle. J Clin Microbiol 1996;34:2170-2174.
30. Goodman JL, Nelson C, Vitale B, et al. Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N Engl J Med 1996;334:209-215. Erratum. N Engl J Med 1996;335:361.
31. Madigan JE, Hietala S, Chalmers S, DeRock E. Seroepidemiologic survey of antibodies to Ehrlichia equi in horses of northern California. J Am Vet Med Assoc 1990;196:1962-1964.
32. 59Fe-labeled erythrocytes in cross-transfused equine blood. Am J Vet Res 1978;39:617-620.
33. Gribble DH. Equine Ehrlichiosis. Davis, CA: University of California; 1970. Thesis.
34. Artursson K, Gunnarsson A, Wikström U-B, Olsson Engvall E. A serological and clinical follow-up in horses with confirmed equine granulocytic ehrlichiosis. Equine Vet J 1999;31:473-477.
35. Madigan JE, Barlough JE, Dumler JS, et al. Equine granulocytic ehrlichiosis in Connecticut caused by an agent resembling the human granulocytotropic Ehrlichia. J Clin Microbiol 1996;34:434-435.
36. Massung RF, Owens JH, Ross D. et al. Sequence analysis of the ank gene of granulocytic ehrlichiae. J Clin Microbiol 2000;38:2917-2922.
37. Madigan JE, Gribble D. Equine ehrlichiosis in northern California: 49 cases (1968-1981). J Am Vet Med Assoc 1987;190:445-448.