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Volumn 44, Issue 6, 2005, Pages 1992-2002

Design of rhenium(I) polypyridine biotin complexes as a new of luminescent probes for avidin

Author keywords

[No Author keywords available]

Indexed keywords

2 (4 HYDROXYPHENYLAZO)BENZOIC ACID; 2,9 DIMETHYL 4,7 DIPHENYL 1,10 PHENANTHROLINE; 3 (N ((2 BIOTINAMIDO)ETHYL)AMIDO)PYRIDINE; 3,4,7,8 TETRAMETHYL 1,10 PHENANTHROLINE; 4 (N ((6 BIOTINAMIDO)HEXANOYL)AMINOMETHYL)PYRIDINE; 4(BIOTINAMIDOMETHYL) PYRIDINE; AVIDIN; BIOTIN DERIVATIVE; LIGAND; PYRIDINE DERIVATIVE; RHENIUM; UNCLASSIFIED DRUG;

EID: 15944412073     PISSN: 00201669     EISSN: None     Source Type: Journal    
DOI: 10.1021/ic049059n     Document Type: Article
Times cited : (78)

References (68)
  • 11
    • 15944411854 scopus 로고    scopus 로고
    • note
    • 2-Cy3 reported in ref 2e is different from common biotin-fluorophore conjugates. The emission of this compound increases at a [Cy3]:[avidin] ratio (n) between 0 and 2. At n = 1, the enhancement factor is about 1.5. However, emission quenching occurs at n > 2. At n = 4, the emission intensity of the solution is about one-half that of the control solutions.
  • 22
    • 11644269152 scopus 로고
    • (a) Lees, A. J. Chem. Rev. 1987, 87, 711-743.
    • (1987) Chem. Rev. , vol.87 , pp. 711-743
    • Lees, A.J.1
  • 51
    • 15944372707 scopus 로고    scopus 로고
    • note
    • 2. These different observations have been discussed in the text.
  • 52
    • 15944420957 scopus 로고    scopus 로고
    • note
    • All of the adducts showed no or very small blue shifts (≤5 nm) in emission wavelength as compared to the corresponding free complexes. Nevertheless, the hydrophobicity associated with a protein molecule in aqueous solution cannot compare to pure dichloromethane. Thus, we suppose that the enhancement of emission intensity and lifetimes of the complexes upon binding to avidin is closely related to an increase in local hydrophobicity.
  • 54
    • 15944384720 scopus 로고    scopus 로고
    • note
    • The rate constants and selected linear plots are included in the Supporting Information.
  • 64
    • 15944422251 scopus 로고    scopus 로고
    • note
    • A lower enhancement factor (1.5) in pure water than in 50 mM phosphate buffer (2.27, Table 3) is probably due to ionic-strength effects. However, a similar enhancement factor (ca. 2.28) was achieved when a more concentrated phosphate buffer (200 mM) was used in the avidin-binding studies. It is unlikely that the enhancement factor would increase further in more concentrated buffers.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.