Real-time polymerase chain reaction quantification of human cytomegalovirus and Epstein-Barr virus in periodontal pockets and the adjacent gingiva of periodontitis lesions

Journal of Periodontal Research

Volumn 40, Issue 2, 2005, Pages 97-104

  Kubar, Ayhan ^a   Saygun, Işil ^a   Özdemir, Atilla ^a   Yapar, Mehmet ^a   Slots, Jörgen ^b  

^a GULHANE SCHOOL OF MEDICINE   (Turkey)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

Aggressive periodontitis; Chronic periodontitis; Cytomegalovirus; Epstein Barr virus; Gingiva; Pathogenesis; Periodontal pocket; Real time polymerase chain reaction; TaqMan

Indexed keywords

NUCLEASE; VIRUS DNA;

ADULT; ARTICLE; CHI SQUARE TEST; CONTROLLED STUDY; CORRELATION COEFFICIENT; CYTOMEGALOVIRUS; DISEASE MARKER; DISEASE SEVERITY; DNA DETERMINATION; EPSTEIN BARR VIRUS; FEMALE; GENE IDENTIFICATION; GINGIVA; HERPES VIRUS; HUMAN; MAJOR CLINICAL STUDY; MALE; MOUTH; PARAMETER; PERIODONTITIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; STATISTICAL SIGNIFICANCE; VIRUS DETECTION; VIRUS GENE; VIRUS ISOLATION; VIRUS LOAD;

ADULT; CHI-SQUARE DISTRIBUTION; CYTOMEGALOVIRUS; DNA, VIRAL; FEMALE; GINGIVA; HERPESVIRUS 4, HUMAN; HUMANS; MALE; MIDDLE AGED; PERIODONTAL POCKET; PERIODONTITIS; POLYMERASE CHAIN REACTION; STATISTICS, NONPARAMETRIC; VIRAL LOAD;

EID: 15744400107     PISSN: 00223484     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0765.2005.00770.x     Document Type: Article

References (40)

1. Page RC, Sturdivant EC. Noninflammatory destructive periodontal disease (NDPD). Periodontol 2000 2002;30:24-39.
2. Socransky SS, Haffajee AD, Goodson JM, Lindhe J. New concepts of destructive periodontal disease. J Clin Periodontol 1984;11:21-32.
3. Mombelli A, Meier C. On the symmetry of periodontal disease. J Clin Periodontol 2001;28:741-745.
4. Slots J. Herpesviruses in periodontal diseases. Periodontol 2000 (in press).
5. Slots J. Update on human cytomegalovirus in destructive periodontal disease. Oral Microbiol Immunol 2004;19:217-223.
6. Kubar A, Saygun I, Yapar M, Özdemir A, Slots J. Real-time PCR quantification of cytomegalovirus in aggressive periodontitis lesions using TaqMan technology. J Periodont Res 2004;39:81-86.
7. Kamma JJ, Contreras A, Slots J. Herpes viruses and periodontopathic bacteria in early-onset periodontitis. J Clin Periodontol 2001;28:879-885.
8. Saygun I, Kubar A, Özdemir A, Yapar M, Slots J. Herpesviral- bacterial interrelationships in aggressive periodontitis. J Periodont Res 2004;39:207-212.
9. Boeckh M, Nichols WG. Immunosuppressive effects of beta-herpesviruses. Herpes 2003;10:12-16.
10. Rush MC, Simon MW. Occurrence of Epstein-Barr virus illness in children diagnosed with group A streptococcal pharyngitis. Clin Pediatr (Phila) 2003;42:417-420.
11. Ting M, Contreras A, Slots J. Herpesvirus in localized juvenile periodontitis. J Periodont Res 2000;35:17-25.
12. Parra B, Slots J. Detection of human viruses in periodontal pockets using polymerase chain reaction. Oral Microbiol Immunol 1996;11:289-293.
13. Saygun I, Yapar M, Özdemir A, Kubar A, Slots J. Human cytomegalovirus and Epstein-Barr virus type 1 in periodontal abscesses. Oral Microbiol Immunol 2004;19:83-87.
14. Idesawa M, Sugano N, Ikeda K et al. Detection of Epstein-Barr virus in saliva by real-time PCR. Oral Microbiol Immunol 2004;19:230-232.
15. Konstantinidis A, Sakellari D, Papa A, Antoniadis A. Real-time PCR quantification of Epstein-Barr virus (EBV) in chronic periodontitis patients. J Periodont Res (in press).
16. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991;88:7276-7280.
17. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996;6:986-994.
18. Silness J, Löe H. Periodontal disease in pregnancy. II. Correlation between oral hygiene and oral condition. Acta Odontol Scand 1964;22:121-135.
19. Löe H. The gingival index, plaque index and the retention index systems. J Periodontol 1967;38:610-616.
20. Ramfjord SP, Nissle RR. The modified widman flap. J Periodontol 1974;45:601-607.
21. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning, Book 3, Appendices B16, 2nd edn. New York: Cold Spring Harbor Laboratory Press, 1989.
22. Kubar A, Yapar M, Besirbellioglu B, Avci IY, Güney C. Rapid and quantitative detection of mumps virus RNA by one-step real-time RT-PCR. Diagn Microbiol Infect Dis 2004;49:83-88.
23. Boeckh M, Boivin G. Quantitation of cytomegalovirus: methodologic aspects and clinical applications. Clin Microbiol Rev 1998;11:533-554.
24. Vieira J, O'Hearn P, Kimball L, Chandran B, Corey L. Activation of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) lytic replication by human cytomegalovirus. J Virol 2001;75:1378-1386.
25. Arcenas R, Widen RH. Epstein-Barr virus reactivation after superinfection of the BJAB-B1 and P3HR-1 cell lines with cytomegalovirus. BMC Microbiol 2002;2:20.
26. Contreras A, Zadeh HH, Nowzari H, Slots J. Herpesvirus infection of inflammatory cells in human periodontitis. Oral Microbiol Immunol 1999;14:206-212.
27. Johannessen AC, Nilsen R, Kristoffersen T, Knudsen GE. Variation in the composition of gingival inflammatory cell infiltrates. J Clin Periodontol 1990;17:298-305.
28. Cobb CM, Ferguson BL, Keselyak NT, Holt LA, MacNeill SR, Rapley JW. A TEM/SEM study of the microbial plaque overlying the necrotic gingival papillae of HIV-seropositive, necrotizing ulcerative periodontitis. J Periodont Res 2003;38:147-155.
29. Liljenberg B, Lindhe J, Berglundh T, Dahlén G, Jonsson R. Some microbiological, histopathological and immunohistochemical characteristics of progressive periodontal disease. J Clin Periodontol 1994;21:720-727.
30. Hill AB. The environment and disease: association or causation? Proc R Soc Med 1965;58:295-300.
31. Slots J, Kamma JJ, Sugar C. The herpesvirus-Porphyromonas gingivalis-periodontitis axis. J Periodont Res 2003;38:318-323.
32. Schenkein HA. Finding genetic risk factors for periodontal diseases: is the climb worth the view? Periodontol 2000 2002;30:79-90.
33. Taylor JJ, Preshaw PM, Donaldson PT. Cytokine gene polymorphism and immunoregulation in periodontal disease. Periodontol 2000 2004;35:158-182.
34. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4:1-6.
35. Slots J, Schonfeld SE. Actinobacillus actinomycetemcomitans in localized juvenile periodontitis, p. 53-64. In: Hamada, S, Holt, SC, McGhee, RJ, eds. Periodontal disease: pathogens and host immune responses. Tokyo, Japan: Quintessence Publishing, 1991.
36. Armitage GC. Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000 2004;34:9-21.
37. Nunn ME. Understanding the etiology of periodontitis: an overview of periodontal risk factors. Periodontol 2000 2003; 32:11-23.
38. Slots J, Contreras A. Herpesviruses: a unifying causative factor in periodontitis? Oral Microbiol Immunol 2000;15:276-279.
39. Yamazaki K, Nakajima T. Antigen specificity and T-cell clonality in periodontal disease. Periodontol 2000 2004;35:75-100.
40. Delaleu N, Bickel M. Interleukin-1 beta and interleukin-18: regulation and activity in local inflammation. Periodontol 2000 2004;35:42-52.