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The azo linkage could also be detected as a broad absorption in the UV-vis spectrum with a maximum at 330 nm. Upon comparison with model compounds prepared with p-cresol, the amount of azo absorption (relative to that of the TMV capsid) was approximately what would be expected for the reported level of conversion. However, this peak was not sufficiently resolved from the protein spectrum to allow quantitative measurement of the conversion by this method.
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See Supporting Information for spectra.
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In the case of rhodamine derivative 7g, UV-vis absorption spectra agreed with the modification ratios reported in the table. Nonetheless, we consider the ESI-MS based ratios to be more accurate. See Supporting Information for details.
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