Characterization of oxidized nicotinamide adenine dinucleotide (NAD +) analogues using a high-pressure-liquid-chromatography-based NAD+-glycohydrolase assay and comparison with fluorescence-based measurements

Analytical Biochemistry

Volumn 340, Issue 1, 2005, Pages 41-51

  Yates, Susan P ^a   Merrill, A Rod ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

HPLC; Mono ADP ribosyltransferase; NAD + glycohydrolase; NAD+ analogues; NAD+ hydrolysis; NADases

Indexed keywords

BINDING ENERGY; CATALYST ACTIVITY; ENZYMES; FLUORESCENCE; HYDROLYSIS; NUCLEOTIDES; TOXIC MATERIALS;

ADP-RIBOSYLTRANSFERASE; GLYCOHYDROLASE; HPLC; NADASES; NAD^+ ANALOGUES;

HIGH PRESSURE LIQUID CHROMATOGRAPHY;

NICOTINAMIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; NICOTINAMIDE ADENINE DINUCLEOTIDE NUCLEOSIDASE; PSEUDOMONAS EXOTOXIN;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; FLUORESCENCE; FLUOROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MATHEMATICAL MODEL; OXIDATION; PRIORITY JOURNAL; PSEUDOMONAS; REVERSED PHASE LIQUID CHROMATOGRAPHY;

PSEUDOMONAS; PSEUDOMONAS EXOTOXIN;

EID: 15544367662     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2005.01.051     Document Type: Article

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