Purification and characterization of jararassin-I, a thrombin-like enzyme from Bothrops jararaca snake venom

Acta Biochimica et Biophysica Sinica

Volumn 36, Issue 12, 2004, Pages 798-802

  Vieira, Débora F ^a   Watanabe, Leandra ^a   Sant'Ana, Carolina D ^b,c   Marcussi, Silvana ^b,c   Sampaio, Suely V ^c   Soares, Andreimar M ^b   Arni, Raghuvir K ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b UNIVERSITY OF RIBEIRÃO PRETO   (Brazil)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Bothrops jararaca; Crystallization; Fibrinogenolytic activity; Serine protease thrombin like; Snake venom

Indexed keywords

BENZAMIDINE; BENZYLSULFONYL FLUORIDE; COAGULATING AGENT; FIBRINOGEN; JARARASSIN I; RESIN; SEPHAROSE; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; SNAKE VENOM; THROMBIN; UNCLASSIFIED DRUG;

ALPHA CHAIN; ARTICLE; BETA CHAIN; BINDING AFFINITY; BLOOD CLOTTING; CHROMATOGRAPHY; CONTROLLED STUDY; COW; CRYSTAL; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; FIBRINOGENOLYSIS; LIGHT SCATTERING; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEFECT; PROTEIN DEGRADATION; QUANTUM YIELD; X RAY DIFFRACTION;

BOTHROPS JARARACA; BOVINAE; SERPENTES;

EID: 15444370005     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: 10.1093/abbs/36.12.798     Document Type: Article

References (20)

1. Mandelbaum FR, Reichel P, Assakura MT. Isolation and characterization of a proteolytic enzyme from the venom of the snake Bothrops jararaca (Jararaca). Toxicon, 1982, 20(6): 955-972
2. Rosenfeld G. In: Bucherl W, Buckley E, Deulofeu V eds. Venomous Animals and Their Venoms II. New York: Academic Press, 1971
3. Selistre HS, Giglio JR. Isolation and characterization of a thrombin-like enzyme from the venom of the snake Bothrops insularis (Jararaca ilhoa). Toxicon, 1987, 25(11): 1135-1144
4. Serrano SM, Mentele R, Sampaio CA, Fink E. Purification, characterization, and amino acid sequence of a serine proteinase, PA-BJ, with platelet-aggregating activity from the venom of Bothrops jararaca. Biochemistry, 1995, 34(21): 7186-7193
5. Matsui T, Fujimura Y, Titani K. Snake venom proteases affecting hemostasis and thrombosis. Biochim Biophys Acta, 2000, 1477(1-2): 146-156
6. Serrano SM, Sampaio CA, Mentele R, Camargo AC, Fink E. A novel fibrinogen-clotting enzyme, TL-BJ, from the venom of the snake Bothrops jararaca: Purification and characterization. Thromb Haemost, 2000, 83(3): 438-444
7. Nishida S, Fujimura Y, Miura S, Ozeki Y, Usami Y, Suzuki M, Titani K et al. Purification and characterization of bothrombin, a fibrinogen-clotting serine protease from the venom of Bothrops jararaca. Biochemistry, 1994, 33(7): 1843-1849
8. Serrano SMT. The isolation and structural and functional characterization of serine proteinases in the venom of Bothrops jararaca. J Venom Anim Toxins, 1999, 5(2): 226
9. Bortoleto RK, Murakami MT, Watanabe L, Soares AM, Ami RK. Purification, characterization and crystallization of jararacussin-I, a fibrinogen-clotting enzyme isolated from the venom of Bothrops jararacussu. Toxicon, 2002, 40(9): 1307-1312
10. Parry MA, Jacob U, Huber R, Wisner A, Bon C, Bode W. The crystal structure of the novel snake venom plasminogen activator TSV-PA: A proto-type structure for snake venom serine proteinases. Structure, 1998, 6(9): 1195-1206
11. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227(5259): 680-685
12. Lopez LM, Sequeiros C, Natalucci CL, Brullo A, Maras B, Barra D, Caffini NO. Purification and characterization of macrodontain I, a cysteine peptidase from unripe fruits of Pseudananas macrodontes (Morr.) harms (Bromeliaceae). Protein Expr Purif, 2000, 18(2): 133-140
13. Astrup T, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys, 1952, 40(2): 346-351
14. Jancarik J, Kim SH. Sparse matrix sampling: A screening method for crystallization of proteins. J Appl Crystallogr, 1991, 24: 409-411
15. Castro HC, Silva DM, Craik C, Zingali RB. Structural features of a snake venom thrombin-like enzyme: thrombin and trypsin on a single catalytic platform? Acta Biochim Biophys, 2001, 1547(2): 183-195
16. Guan LF, Chi CW, Yuan M. Study on the thrombin-like enzyme preferentially releasing fibrinopeptide B from the snake venom of Agkistrodon halys Pallas. Thromb Res, 1984, 35: 301-310
17. Pirkle H. Thrombin-like enzymes from snake venoms: An updated inventory. Thromb Haemost, 1998, 79(3): 675-683
18. Matsui T, Sakurai Y, Fujimura Y, Hayashi I, Oh-Ishi S, Suzuki M, Hamako J et al. Purification and amino acid sequence of halystase from snake venom of Agkistrodon halys blomhoffii, a serine protease that cleaves specifically fibrinogen and kininogen. Eur J Biochem, 1998, 252(3): 569-575
19. Markland FS. Snake venoms and the hemostatic system. Toxicon, 1998, 36(12): 1749-1800
20. Marsh NA. Use of snake venom fractions in the coagulation laboratory. Blood Coagul Fibrinolysis, 1998, 9(5): 395-404