-
4
-
-
0001872370
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-
M. Freeling and V. Walbot, Eds. Springer-Verlag, New York
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P. Chomet, in The Maize Handbook, M. Freeling and V. Walbot, Eds. (Springer-Verlag, New York, 1994), pp. 243-248.
-
(1994)
The Maize Handbook
, pp. 243-248
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-
Chomet, P.1
-
6
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0028848836
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-
M. Frey, R. Kliem, H. Saedler, A. Gierl, Mol. Gen. Genet. 246, 100 (1995). The new gene designation is as follows: B×2 = Cyp71C4, B×3 = Cyp71C2, B×4 = Cyp71C1, and B×S = Cyp71C3.
-
(1995)
Mol. Gen. Genet.
, vol.246
, pp. 100
-
-
Frey, M.1
Kliem, R.2
Saedler, H.3
Gierl, A.4
-
7
-
-
0028834605
-
-
Individual plants were analyzed by PCR amplification of DNA restriction fragments ligated to an adapter, similar to the amplified fragment length polymorphism (AFLP) protocol [P. Vos et al., Nucleic Acids Res. 23, 4407 (1995)]; however, a Mu-specific biotinylated primer [AGAGAAGCCAACGCCA(A/T)C-GCCTCCATT] was used to isolate Mu-sequences.
-
(1995)
Nucleic Acids Res.
, vol.23
, pp. 4407
-
-
Vos, P.1
-
8
-
-
15444356183
-
-
note
-
The maize inbred line C31A was the source for all wild-type cDNA and genomic clones. Cloning was performed as described (6).
-
-
-
-
10
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-
0024297340
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-
C. C. Hyde, S. A. Ahmed, E. A. Padlan, E. W. Miles, D. R. Davies, J. Biol. Chem. 263, 17857 (1988).
-
(1988)
J. Biol. Chem.
, vol.263
, pp. 17857
-
-
Hyde, C.C.1
Ahmed, S.A.2
Padlan, E.A.3
Miles, E.W.4
Davies, D.R.5
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12
-
-
0014748044
-
-
Isolation of indole-3-glycerol phosphate and fluorimetric enzymatic assay were done as described [T. E. Creighton, Eur. J. Biochem. 13, 1 (1970)]. A reaction volume of 1 ml containing 4 μg of BX1 protein, 50 μg of glyceraldehyde phosphate dehydrogenase, and 0.5 mM nicotinamide adenine dinucleotide (oxidized form) was incubated at 22°C for 2 min. Indole-3-glycerol phosphate concentration varied from 0 to 50 μM. The identity of the product indole was proven by Ehrlich's reagent [W. K. Lim et al., J. Bacteriol. 173, 1886 (1991)]. BX1 protein was expressed in E. coli by inserting the cDNA into a modified pET3a vector [A. H. Rosenberg et al., Gene 56, 125 (1987)] and was purified to homogeneity by means of a six-nucleotide oligomer COOH-terminal histidine tag (Qiagen Ni-NTA purification system).
-
(1970)
Eur. J. Biochem.
, vol.13
, pp. 1
-
-
Creighton, T.E.1
-
13
-
-
0026035205
-
-
Isolation of indole-3-glycerol phosphate and fluorimetric enzymatic assay were done as described [T. E. Creighton, Eur. J. Biochem. 13, 1 (1970)]. A reaction volume of 1 ml containing 4 μg of BX1 protein, 50 μg of glyceraldehyde phosphate dehydrogenase, and 0.5 mM nicotinamide adenine dinucleotide (oxidized form) was incubated at 22°C for 2 min. Indole-3-glycerol phosphate concentration varied from 0 to 50 μM. The identity of the product indole was proven by Ehrlich's reagent [W. K. Lim et al., J. Bacteriol. 173, 1886 (1991)]. BX1 protein was expressed in E. coli by inserting the cDNA into a modified pET3a vector [A. H. Rosenberg et al., Gene 56, 125 (1987)] and was purified to homogeneity by means of a six-nucleotide oligomer COOH-terminal histidine tag (Qiagen Ni-NTA purification system).
-
(1991)
J. Bacteriol.
, vol.173
, pp. 1886
-
-
Lim, W.K.1
-
14
-
-
0023464708
-
-
Isolation of indole-3-glycerol phosphate and fluorimetric enzymatic assay were done as described [T. E. Creighton, Eur. J. Biochem. 13, 1 (1970)]. A reaction volume of 1 ml containing 4 μg of BX1 protein, 50 μg of glyceraldehyde phosphate dehydrogenase, and 0.5 mM nicotinamide adenine dinucleotide (oxidized form) was incubated at 22°C for 2 min. Indole-3-glycerol phosphate concentration varied from 0 to 50 μM. The identity of the product indole was proven by Ehrlich's reagent [W. K. Lim et al., J. Bacteriol. 173, 1886 (1991)]. BX1 protein was expressed in E. coli by inserting the cDNA into a modified pET3a vector [A. H. Rosenberg et al., Gene 56, 125 (1987)] and was purified to homogeneity by means of a six-nucleotide oligomer COOH-terminal histidine tag (Qiagen Ni-NTA purification system).
-
(1987)
Gene
, vol.56
, pp. 125
-
-
Rosenberg, A.H.1
-
16
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-
0026871098
-
-
A. D. Wright, C. A. Moehlenkamp, G. H. H. Perrot, M. G. Neuffer, K. C. Cone, Plant Cell 4, 711 (1992).
-
(1992)
Plant Cell
, vol.4
, pp. 711
-
-
Wright, A.D.1
Moehlenkamp, C.A.2
Perrot, G.H.H.3
Neuffer, M.G.4
Cone, K.C.5
-
17
-
-
0030976838
-
-
E. Glawischnig, W. Eisenreich, A. Bacher, M. Frey, A. Gierl, Phytochemistry 45, 715 (1997).
-
(1997)
Phytochemistry
, vol.45
, pp. 715
-
-
Glawischnig, E.1
Eisenreich, W.2
Bacher, A.3
Frey, M.4
Gierl, A.5
-
18
-
-
0029167503
-
-
1 maize plants mutagenized by means of a Robertson's Mutator element was screened for Mu-containing alleles of the P-450 genes by a reverse genetics-based technology [R. J. Bensen et al., Plant Cell 7, 75 (1995)]. PCR amplifications were done as described [M. Mena et al., Science 274, 1537 (1996)].
-
(1995)
Plant Cell
, vol.7
, pp. 75
-
-
Bensen, R.J.1
-
19
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0029807048
-
-
1 maize plants mutagenized by means of a Robertson's Mutator element was screened for Mu-containing alleles of the P-450 genes by a reverse genetics-based technology [R. J. Bensen et al., Plant Cell 7, 75 (1995)]. PCR amplifications were done as described [M. Mena et al., Science 274, 1537 (1996)].
-
(1996)
Science
, vol.274
, pp. 1537
-
-
Mena, M.1
-
20
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0027480346
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G. Truan et al., Gene 125, 49 (1993).
-
(1993)
Gene
, vol.125
, pp. 49
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Truan, G.1
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21
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0028357263
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P. Urban et al., Eur. J. Biochem. 222, 843 (1994). Full-size P-450 cDNAs were inserted in the expression vector, beginning with the AUG translation start codon. This was accomplished by PCR amplification of the relevant cDNA sequences.
-
(1994)
Eur. J. Biochem.
, vol.222
, pp. 843
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-
Urban, P.1
-
22
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0029776005
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D. Pompon, B. Louerat, A. Bronine, P. Urban, Methods Enzymol. 272, 51 (1996).
-
(1996)
Methods Enzymol.
, vol.272
, pp. 51
-
-
Pompon, D.1
Louerat, B.2
Bronine, A.3
Urban, P.4
-
23
-
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15444352737
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-
note
-
1H NMR (Table 1).
-
-
-
-
25
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-
0002867460
-
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2-DIBOA, m/e values were 325 (31%), 310 (84%), 297 (17%), 208 (43%), 192 (54%), 191 (30%), 179 (36%), 164 (72%), 151 (24%), 150 (23%), 147 (100%), and 136 (73%).
-
(1979)
Plant Physiol.
, vol.63
, pp. 9
-
-
Woodward, M.D.1
Corcuera, L.J.2
Schnoes, H.K.3
Helgeson, J.P.4
Upper, C.D.5
-
31
-
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15444340162
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-
note
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We used gradient-enhanced heteronuclear multiple-quantum coherence, heteronuclear multiple-bond correlation, and double-quantum filtered correlation spectroscopy techniques (M. Frey et al., data not shown).
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-
-
-
32
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15444342732
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note
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-13C]indole; M. H. Zenk, T. M. Kutchan, and K. Kirschner for discussions and suggestions; J. Winkler and H. Krause for GC-MS analysis; and P. Urban and D. Pompon for the yeast expression system. Supported by Deutsche Forschungsgemeinschaft grant SFB 369 and by Fonds der Chemischen Industrie.
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