Diversification of C-function activity in maize flower development

Science

Volumn 274, Issue 5292, 1996, Pages 1537-1540

  Mena, Montaña ^a,c   Ambrose, Barbara A ^a   Meeley, Robert B ^b   Briggs, Steven P ^b   Yanofsky, Martin F ^a   Schmidt, Robert J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b PIONEER HI BRED INTERNATIONAL INC   (United States)

^c UNIVERSIDAD POLITÉCNICA DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

PLANT RNA;

ARTICLE; GENE EXPRESSION; GENE MUTATION; MAIZE; MOLECULAR CLONING; NONHUMAN; PHENOTYPE; PLANT GROWTH; PRIORITY JOURNAL; REPRODUCTION; SCANNING ELECTRON MICROSCOPY; SEQUENCE HOMOLOGY; SEX DIFFERENTIATION; TRANSPOSON;

ALLELES; AMINO ACID SEQUENCE; DNA TRANSPOSABLE ELEMENTS; DNA-BINDING PROTEINS; GENE EXPRESSION; GENES, PLANT; MADS DOMAIN PROTEINS; MICROSCOPY, ELECTRON, SCANNING; MOLECULAR SEQUENCE DATA; MORPHOGENESIS; MUTATION; PHENOTYPE; PLANT PROTEINS; RNA, MESSENGER; RNA, PLANT; TRANSCRIPTION FACTORS; ZEA MAYS;

ARABIDOPSIS; ZEA MAYS;

EID: 0029807048     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5292.1537     Document Type: Article

References (28)

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9. 1 maize plants mutagenized by means of a Robertson's Mutator element was screened for Mu-containing alleles of the ZAG1 gene by a reverse genetics-based technology [R. J. Bensen et al., Plant Cell 7, 75 (1995)]. Polymerase chain reactions (PCRs) were initially performed on pooled and subsequently on individual DNA samples using a Mu-specific primer [5′-CCCT-GAGCTCTTCGTC(CT)ATAATGGCAATTATCTC-3′] to the conserved region of the Mu terminal inverted repeat in combination with four different primers specific for ZAG1 exon sequences (5). Cloning and sequencing of the corresponding amplified PCR products confirmed that one family (1482A2) carried a Mu element inserted in the ZAG1 gene. RNA isolation, blotting, and hybridization procedures were performed as described (5). ZAG1-5′ encompasses nucleotides 1 to 236; ZAG1-3′ spans nucleotides 716 to 936 immediately 3′ of the Mu insertion.
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25. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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27. Analysis of RNA expression was performed as described in Fig. 1. A 500-base pair (bp) Hpa I-Nde I fragment was used as the ZAG1 probe in Fig. 4B. The ZMM2-specific probe (see below) was a 400-bp Xho I fragment isolated from the 3′ end of the cDNA. Both probes were labeled to comparable specific activities. The faint ZMM2 hybridization detected in endosperm is likely a result of nucellar tissue, which often contaminates endosperm tissue obtained from early developmental stages. RNA from immature ears and tassels was isolated from developing inflorescences that were <3 cm in length. Hybridization with a tubulin probe produced signal in every lane.
28. We thank T. Fox and M. Albertson for use of their tassel cDNA library. Supported in part by postdoctoral fellowships from the North Atlantic Treaty Organization and Ministerio de Educación y. Ciencia (to M,M.) and from the Pioneer Discovery Research Program (to R.B.M.) and by grants from the U.S. Department of Agriculture and the University of California Biotechnology and Research Foundation (to R.J.S. and M.F.Y.).