Two phenotypically compensating isocitrate dehydrogenases in Ralstonia eutropha

FEMS Microbiology Letters

Volumn 227, Issue 1, 2003, Pages 9-16

  Wang, Zheng Xiang ^a,b   Brämer, Christian ^a   Steinbüchel, Alexander ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b JIANGNAN UNIVERSITY   (China)

Author keywords

Isocitrate dehydrogenase; Isocitrate lyase; Malate synthase; Polyhydroxyalkanoic acids; Ralstonia eutropha

Indexed keywords

ACETIC ACID; ISOCITRATE DEHYDROGENASE; POLY(3 HYDROXYBUTYRIC ACID); ISOENZYME;

ARTICLE; ESCHERICHIA COLI; GENE MUTATION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; WAUTERSIA EUTROPHA; WILD TYPE; AMINO ACID SEQUENCE; CITRIC ACID CYCLE; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY;

ESCHERICHIA COLI; WAUTERSIA EUTROPHA;

AMINO ACID SEQUENCE; CITRIC ACID CYCLE; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; ISOCITRATE DEHYDROGENASE; ISOENZYMES; MOLECULAR SEQUENCE DATA; PHENOTYPE;

EID: 1542543656     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00612-8     Document Type: Article

References (33)

1. Walsh, K. and Koshland, D.E. (1985) Branch point control by the phosphorylation state of isocitrate dehydrogenase: a quantitative examination of fluxes during a regulatory transition. J. Biol. Chem. 260, 8430-8437.
2. Holms, W.H. (1986) The central metabolic pathways of Escherichia coli: relationship between flux and control at a branch point, efficiency of conversion of biomass, and excretion of acetate. Curr. Top. Cell. Regul. 28, 69-105.
3. LaPorte, D.C. and Koshland, D.E.J. (1982) A protein with kinase and phosphatase activities involved in regulation of tricarboxylic acid cycle. Nature 300, 458-460.
4. Chen, R.-D. and Gadal, P. (1990) Structure, functions and regulation of NAD and NAPD dependent isocitrate dehydrogenase in higher plants and in other organisms. Plant Physiol. Biochem. 28, 411-427.
5. Glaeser, H. and Schlegel, H.G. (1972) Synthese der Enzyme des Tricarbonsäure-Cyclus in Hydrogenomonas eutropha Stamm H16. Arch. Mikrobiol. 86, 315-325.
6. Schlegel, H.G., Kaltwasser, H. and Gottschalk, G. (1961) Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 38, 209-222.
7. Sambrook, J., Fritsch, E. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
8. Marmur, J. (1961) A procedure for the isolation of desoxyribonucleic acids from microorganisms. J. Mol. Biol. 3, 208-218.
9. Birnboim, H.C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513-1523.
10. Hohn, B. and Murray, K. (1977) Packaging recombinant DNA molecules into bacteriophage particles in vitro. Proc. Natl. Acad. Sci. USA 74, 3259-3263.
11. Brämer, C.O. and Steinbüchel, A. (2001) The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate metabolism. Microbiology 147, 2203-2214.
12. Grunstein, M. and Hogness, D.S. (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA 72, 3961-3965.
13. Strauss, E.C., Kobori, J.A., Siu, G. and Hood, L.E. (1986) Specific-primer-directed DNA sequencing. Anal. Biochem. 154, 353-360.
14. Overhage, J., Priefert, H., Rabenhorst, J. and Steinbüchel, A. (1999) Biotransformation of eugenol to vanillin by a mutant of Pseudomonas sp. strain HR199 constructed by disruption of the vanillin dehydrogenase (vdh) gene. Appl. Microbiol. Biotechnol. 52, 820-828.
15. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
16. Timm, A., Byrom, D. and Steinbüchel, A. (1990) Formation of blends of various poly(3-hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans. Appl. Microbiol. Biotechnol. 33, 296-301.
17. Hurley, J.H., Thorsness, P.E., Ramalingam, V., Helmers, N.H., Koshland, D.E. and Stroud, R.M. (1989) Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase. Proc. Natl. Acad. Sci. USA 86, 8635-8639.
18. Chung, A.E. and Franzen, J.S. (1969) Oxidized triphosphopyridine nucleotide specific isocitrate dehydrogenase from Azotobacter vinelandii: purification and characterization. Biochemistry 8, 3175-3184.
19. Leyland, M.L. and Kelly, D. (1991) Purification and characterization of a monomeric isocitrate dehydrogenase with dual coenzyme specificity from the photosynthetic bacterium, Rhodomicrobium vannielii. Eur. J. Biochem. 202, 85-93.
20. Sahara, T., Suzuki, M., Tsuruha, J.I., Takada, Y. and Fukunaga, N. (1999) Cis-acting elements responsible for low-temperature-inducible expression of the gene coding for the thermolabile isocitrate dehydrogenase isozyme of a psychrophilic bacterium, Vibrio sp. strain ABE-1. J. Bacteriol. 181, 2602-2611.
21. Stover, C.K., Pham, X.Q., Erwin, A.L., Mizoguchi, S.D., Warrener, P., Hickey, M.J., Brinkman, F.S., Hufnagle, W.O., Kowalik, D.J., Lagrou, M., Garber, R.L., Goltry, L., Tolentino, E., Westbrock-Wadman, S., Yuan, Y., Brody, L.L., Coulter, S.N., Folger, K.R., Kas, A., Larbig, K., Lim, R., Smith, K., Spencer, D., Wong, G.K., Wu, Z. and Paulsen, I.T. (2000) Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406, 959-964.
22. Salanoubat, M., Genin, S., Artiguenave, F., Gouzy, J., Mangenot, S., Arlat, M., Billault, A., Brottier, P., Camus, J.C., Cattolico, L., Chandler, M., Choisne, N., Claudel-Renard, C., Cunnac, S., Demange, N., Gaspin, C., Lavie, M., Moisan, A., Robert, C., Saurin, W., Schiex, T., Siguier, P., Thebault, P., Whalen, M., Wincker, P., Levy, M., Weissenbach, J. and Boucher, C.A. (2002) Genome sequence of the plant pathogen Ralstonia solanacearum. Nature 415, 497-502.
23. Chung, T., Klumpp, D.J. and LaPorte, D.C. (1988) Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map. J. Bacteriol. 170, 386-392.
24. Madison, L.L. and Huisman, G.W. (1999) Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63, 21-53.
25. Klinke, S., Dauner, M., Scott, G., Kessler, B. and Witholt, B. (2000) Inactivation of isocitrate lyase leads to increased production of medium-chain-length poly(3-hydroxyalkanoates) in Pseudomonas putida. Appl. Environ. Microbiol. 66, 909-913.
26. Park, J.S. and Lee, Y.H. (1996) Metabolic characteristics of isocitrate dehydrogenase leaky mutant of Alcaligenes eutrophus and its utilization for poly-β-hydroxybutyrate production. J. Ferment. Bioeng. 81, 197-205.
27. Srivastava, S., Urban, M. and Friedrich, B. (1982) Mutagenesis of Alcaligenes eutrophus by insertion of the drug-resistance transposon Tn5. Arch. Microbiol. 131, 203-207.
28. Bullock, W.O., Fernandez, J.M. and Stuart, J.M. (1987) XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. Biotechniques 5, 376-379.
29. Simon, R., Priefer, U. and Pühler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1, 781-791.
30. Hohn, C. and Collins, J. (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11, 291-298.
31. Simon, R., Priefer, U. and Pühler, A. (1983) Vector plasmids for in vivo and in vitro manipulations of Gram negative bacteria. In: Molecular Genetics of the Bacteria/plant Interaction (Pühler, A., Ed.). Springer, Berlin.
32. Manchak, J. and Page, W.J. (1994) Control of polyhydroxyalkanoate synthesis in Azotobacter vinelandii stain UWD. Microbiology 140, 953-963.
33. Carlson, R., Fell, D. and Srienc, F. (2002) Metabolic pathway analysis of a recombinant yeast for rational strain development. Biotechnol. Bioeng. 79, 121-134.