Metabolic characteristics of isocitrate dehydrogenase leaky mutant of Alcaligenes eutrophus and its utilization for poly-β-hydroxybutyrate production

Journal of Fermentation and Bioengineering

Volumn 81, Issue 3, 1996, Pages 197-205

  Park, Jin Seo ^a   Lee, Yong Hyun ^a  

^a Kyungpook National University   (South Korea)

Author keywords

Alcaligenes eutrophus; isocitrate dehydrogenase leaky mutant; metabolic characteristics; poly(3 hydroxybutyrate 3 hydroxyvalerate; poly hydroxybutyrate; production

Indexed keywords

ISOCITRATE DEHYDROGENASE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL METABOLISM; ENZYME ISOLATION; FERMENTATION; MUTAGENESIS; NONHUMAN; WAUTERSIA EUTROPHA;

EID: 0029969492     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0922-338X(96)82208-2     Document Type: Article

References (29)

1. Dawes, E. A. and Senior, P.J.: The role and regulation of energy reserve polymers in microorganisms. Adv. Microbiol. Physiol., 10, 135-266 (1973).
2. Holmes, P. A.: Applications of PHB, a microbially produced biodegradable thermoplastic. Phys. Technol., 16, 32-36 (1985).
3. Jackson, F. A. and Dawes, E. A.: Regulation of the tricarboxylic acid cycle and poly-β-hydroxybutyrate metabolism in Azotobacter beijerlinkii grown under nitrogen or oxygen limitation. J. Gen. Microbiol., 97, 303-312 (1976).
4. Oeding, V. and Schlegel, H.G.: β-Ketothiolase from Hydrogenomonas eutropha H16 and its significance in the regulation of poly-β-hydroxybutyrate metabolism. Biochem. J., 134, 239-248 (1973).
5. Senior, P. J. and Dawes, E. A.: Poly-β-hydroxybutyrate biosynthesis and the regulation of glucose metabolism in Azotobacter eijerlinkii. Biochem. J., 125, 55-66 (1971).
6. King, P. P.: Biotechnology: an industrial view. J. Chem. Tech. Biotechnol., 32, 2-8 (1982).
7. Byrom, D.: Polymer synthesis by microorganisms, technology and economics. Trends Biotechnol., 5, 246-250 (1987).
8. Kirn, H.-Y., Park, J.-S., Shin, H.-D., and Lee, Y.-H.: Isolation of glucose utilizing mutant of Alcaligenes eutrophus, its substrate selectivity, and accumulation of poly-β-hydroxybutyrate. Kor. J. Microbiol., 33, 51-58 (1995).
9. Kim, G.-T., Park, J.-S., Park, H.-C., Lee, Y.-H., and Huh, T.-L.: Construction of the recombinant phbCAB operon of Alcaligenes eutrophus for accumulation of poly-β-hydroxybutyric acid in Escherichia coli. Kor. J. Appl. Microbiol. Biotechnol., 21, 221-228 (1993).
10. Park, H.-C., Lim, K.-J., Park, J.-S., Lee, Y.-H., and Huh, T.-L.: High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation. Biotechnol. Tech., 9, 31-34 (1995).
11. Park, H.-C., Park, J.-S., Lee, Y.-H., and Huh, T.-L.: Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus. Biotechnol. Lett., 17, 729-734 (1995).
12. Park, J.-S., Park, H.-C., Huh, T.-L., and Lee, Y.-H.: Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus transformants harbouring cloned phbCAB genes. Biotechnol. Lett., 17, 735-740 (1995).
13. Peoples, O.P. and Sinskey, A.J.: Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16, characterization of the genes encoding β-ketothiolase and acetoacetyl-CoA reduc- tase. J. Biol. Chem., 264, 15293-15297 (1989).
14. Senior, P. J. and Dawes, E.A.: The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerlinkii. Biochem. J., 134, 225-238 (1973).
15. Deutsch, J.: Glucose-6-phosphate dehydrogenase from yeast, p. 195-196. In Bergmeyer, J. and Graβ1, D. (ed.), Methods of enzymatic analysis, vol. 2, 3rd ed. Verlag Chemie, Weinheim (1983).
16. Goldberg, D. M. and Ellis, G.: Isocitrate dehydrogenase, p. 183-190. In Bergmeyer, J. and Graβ1, D. (ed.), Methods of enzymatic analysis, vol. 3, 3rd ed. Verlag Chemie, Weinheim (1983).
17. Fahlen, L. A., Wigger, B. O., and Cohen, P. P.: Glutamate dehydrogenase from bovine liver, p. 208-209. In Bergmeyer, J. and Graβ1, D. (ed.), Methods of enzymatic analysis, vol. 3, 3rd ed. Verlag Chemie, Weinheim (1983).
18. Christian, B. A.: Aconitase from pig heart muscle, p. 695-698. In Colowick, S. P. and Kaplan, N. O. (ed.), Methods in enzymology, vol. 1, 1st ed. Academic Press Inc., New York (1968).
19. Berger, E. and Chavarie, C.: PHB recovery by hypochlorite digestion of non-PHB biomass. Biotechnol. Tech., 3, 227-232 (1989).
20. Law, J. H. and Slepecky, R. A.: Assay of poly-β-hydroxybutyric acid. J. Bacteriol., 82, 33-36 (1960).
21. Braunegg, G., Sonnleitner, B., and Laffery, R. M.: A rapid gas Chromatographie method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol., 6, 29-37 (1978).
22. Miller, G. L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426-428 (1959).
23. Ryu, D. Y. and Chung, S. H.: Performance of the continuous glucose isomerase reactor system for the production of fructose syrup. Biotechnol. Bioeng., 19, 159-184 (1977).
24. Bradford, M. M.: A rapid and sensitive method for the quantitation of microorganism quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
25. Yamane, T.: Yield of poly-D(-)-hydroxybutyrate from various carbon sources: a theoretical study. Biotechnol. Bioeng., 41, 165-170 (1993).
26. Sawyer, M. H., Baumann, P., and Banmann, L.: Pathway of D-fructose and D-glucose catabolism in marine species of Alcaligenes, Pseudomonas mariana, and Alteromonas communis. Arch. Microbiol., 112, 169-172 (1977).
27. Vollbracht, D., El Nawawy, M. A., and Schlegel, H. G.: Excretion of metabolites by hydrogen bacteria, I. Autrophic and heterotrophic fermentations. Eur. J. Appl. Microbiol., 6, 145-155 (1978).
28. Eklund, T.: Inhibition of microbial growth at different pH levels by benzoic and propionic acid and esters of p-hydroxybenzoic acid. Int. J. Food. Microbiol., 2, 159-167 (1985).
29. Doi, Y., Takami, A., Kunioka, M., and Soga, K.: Production of copolyesters of 3-hydroxybutyrate and 3-hydroxyvalerate by Alcaligenes eutrophus from butyric and pentanoic acids. Appl. Microbiol. Biotechnol., 28, 330-334 (1988).