Radiation protection by disulfiram: Protection of membrane and DNA in vitro and in vivo against γ-radiation

Journal of Radiation Research

Volumn 44, Issue 3, 2003, Pages 255-259

  Gandhi, Nitin Motilal ^a   Gopalaswamy, Usulumarty Venu ^a   Nair, Cherupally Krishnan K ^a  

^a BHABHA ATOMIC RESEARCH CENTRE   (India)

Author keywords

Comet assay; Disulfiram; Lipid peroxidation; Radioprotector

Indexed keywords

BIOMOLECULES; CELL CULTURE; ELECTROPHORESIS; GAMMA RAYS; LIPIDS; MAMMALS; MEMBRANES; OXIDATION; RADIATION PROTECTION;

% REDUCTIONS; COMET ASSAYS; DISULFIRAM; IN-VITRO; LIPID PEROXIDATION; MEMBRANE LIPIDS; PLASMID DNA; RADIATION-INDUCED DAMAGES; RADIOPROTECTORS;

DNA;

DISULFIRAM; DNA; HYDROXYL RADICAL; RADIOPROTECTIVE AGENT; THIOBARBITURIC ACID REACTIVE SUBSTANCE; MEMBRANE LIPID;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL DAMAGE; CELL PROTECTION; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; FENTON REACTION; GAMMA RADIATION; IN VITRO STUDY; IN VIVO STUDY; LIPID PEROXIDATION; MALE; MICROSOME MEMBRANE; MOUSE; NONHUMAN; OXIDATIVE STRESS; PLASMID; RADIATION DOSE; RADIATION EXPOSURE; RADIATION INJURY; RADIATION PROTECTION; RAT; ANIMAL; CELL MEMBRANE; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECT; LIVER MICROSOME; METABOLISM; METHODOLOGY; RADIATION RESPONSE; WISTAR RAT;

ANIMALIA;

ANIMALS; CELL MEMBRANE; DISULFIRAM; DNA; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; DOSE-RESPONSE RELATIONSHIP, RADIATION; GAMMA RAYS; LIPID PEROXIDATION; MALE; MEMBRANE LIPIDS; MICE; MICROSOMES, LIVER; RADIATION DOSAGE; RADIATION PROTECTION; RADIATION-PROTECTIVE AGENTS; RATS; RATS, WISTAR;

EID: 1542441532     PISSN: 04493060     EISSN: None     Source Type: Journal    
DOI: 10.1269/jrr.44.255     Document Type: Article

References (24)

1. Nair, C. K. K., Parida, D. K. and Nomura, T. (2001) Radioprotectors in radiotherapy. J. Radiat. Res. 42: 129-137.
2. Fuller, R., Branchey, L., Brightwell, D. et al. (1986) Disulfiram treatment of alcoholism. JAMA 256: 1449-1455.
3. Bertram, B., Schuhmacher, J., Frei, E., Frank, N. and Wiessler, M. (1982) Effects of dilsufiram on mixed function oxidase system and trace element concentration in the liver of rats. Biochem. Pharmacol. 31: 3613-3619.
4. Calle, L. M. and Sullivan, P. D. (1982) Screening of antioxidants and other compounds for antimutagenic properties toward benzo(a)pyrene-induced mutagenicity in strain TA98 of Salmonella typhimurium. Mutat. Res. 101: 99-104.
5. Gopalaswamy, U. V., Frei, E., Frank, N., Kliem, H. C., Wiessler, M., Bertram, B. and Bhattacharya, R. K. (1998) Chemopreventive effects of dithiocarbamates on aflatoxin B1 metabolism and formation of AFB1 adducts with glutathione. Anticancer Res. 18: 1827-1832.
6. Dunsford, H. A., Dolan, P. M., Seed, J. E. and Bueding, E. (1984) Effects of multiple putative anticarcinogenes on the carcinogenicity of trans-5-amino-3-(2-(5-nitro-2furyl)vinyl)-1,2,4-oxadiazole. J. Natl. Cancer Inst. 73: 161-168.
7. Bertram, B., Frei, E. and Wiessler, M. (1985) Influence of disulfiram on glutathione, glutathione-S-transferase, and on nitrosamine dealkylases of liver, kidney and esophagus of the rat. Biochem. Pharmacol. 34: 387-388.
8. Marcelos, M., Lang, M. and Torronen, R. (1976) Modifications of drug metabolism by disulfiram and diethyl dithiocarbamate on D-glucoronic acid pathway. Chem. Biol. Interact. 15: 277-287.
9. Kyle, M. E., Serroni, A. and Farber, J. L. (1989) The inhibition of lipid peroxidation by disulfiram prevents the killing of cultured hepatocytes by allyl alcohol, tert-butyl hydroperoxide, hydrogen peroxide and diethyl maleate. Chem.-Biol. Interact. 72: 269-273.
10. Hausknecht, E. C. and Funk, M. O. (1984) The differential effect of disulfiram on lipooxygenases (EC 1.13.11.12) from Glycine max. Phytochemistry 23: 1535-1540.
11. Nabekura, Y., Ito, Y., Cai, H., Terao, M. and Hori, R. (2000) Effect of lipid composition on the transcorneal penetration of liposomes containing disulfiram, a potent anti-cataract agent, in the rabbit. Biol. Pharm. Bull. 23: 327-332.
12. Shah, G. M. and Bhattacharya, R. K. (1982) In vivo effect of L-ascorbic acid on benzo(a)pyrene metabolite-DNA adduct formation in rat liver. J. Biosci. 4: 263-26.
13. Lowry, O. H., Rosenblum, N. J., Farr, A. L. and Randall, J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275.
14. Buege, A. J. and Aust, S. D. (1978) Microsomal lipid peroxidation. In: Methods in Enzymology, Eds. S. Fleischer and L. Packer, Vol. 52, pp. 302-310, Academic Press, New York.
15. Sambrook, K. J., Fritsch, J. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Mannual, 2nd ed., p. 6.
16. Rajgopalan, R., Khalida, W., Huilgol, N. G., Kagiya, V. T. and Nair, C. K. K. (2002) Inhibition of γ-radiation induced DNA damage in plasmid pBR322 by TMG, a water soluble derivative of vitamin E. J. Radiat. Res. 43: 153-159.
17. Elizabeth, K. and Rao, M. N. A. (1990) Oxygen radical scavenging activity of curcumin. Int. J. Pharm. 58: 237-240.
18. Cheng, Z., Li, Y. and Chang, W. (2003) Kinetic deoxyribose degradation assay and its application in assessing the antioxidant activities of phenolic compounds in a Fenton-type reaction system. Anal. Chim. Acta 478: 129-137.
19. Gajendiran, N., Tanaka, K. and Kamada, N. (2001) Comet assay to assess the non-target effect of neutron-radiation in human peripheral blood. J. Radat. Res. 42: 157-163.
20. Sonntag, C. V. (1987) The chemical basis of radiation biology, Taylor and Francis, London
21. Phillips, M., Agarwal, R. P., Brodeau, J., Garagusi, V. F. and Mossman, K. L. (1985) Stability of an injectable disulfiram formulation sterilised by gamma radiation. Am. J. Hosp. Pharm. 42: 343-345.
22. Foye, W. O. (2000) History of Radioprotector Development. In: Radioprotector, Chemical, Biological and Clinical Perspectives, Eds. E. A. Bump, and K. Malekar, pp. 15-24, CRC Press.
23. Madrigal-Bujaidar, E., Valazquez-Guadarrama, N., Morales-Ramirez, P. and Mendiola, M. T. (2002) Effect of disulfiram on the genotoxic potential of acetaldehyde in mouse spermatogonial cells. Teratogen. Carcinogen. Mutagen. 22: 83-91.
24. Brewer, C., Meyers, R. J. and Johnsen, J. (2000) Does disulfiram help to prevent relapse in Alcohol abuse? CNS Drugs 14: 329-341.