Membrane transport of fatty acylcarnitine and free L-carnitine by rat liver microsomes

European Journal of Biochemistry

Volumn 271, Issue 5, 2004, Pages 954-961

  Gooding, Jason M ^a   Shayeghi, Majid ^a   Saggerson, E David ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Acylcarnitine; Carnitine; Liver; Microsomes; Transport

Indexed keywords

ACYL COENZYME A; ACYLTRANSFERASE; CARNITINE; CARNITINE ACYLTRANSFERASE; CARRIER PROTEIN; FATTY ACID; ION CHANNEL; MERSALYL; N ETHYLMALEIMIDE; PALMITOYL COENZYME A; PALMITOYLCARNITINE;

ANIMAL CELL; ARTICLE; CELL MEMBRANE TRANSPORT; CONTROLLED STUDY; ENZYME ANALYSIS; FATTY ACID TRANSPORT; LIVER MICROSOME; MALE; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; BIOLOGICAL TRANSPORT; CARNITINE; ENDOPLASMIC RETICULUM; ENZYME INHIBITORS; GENES, REPORTER; INTRACELLULAR MEMBRANES; MALE; MERSALYL; MICROSOMES, LIVER; PALMITOYL COENZYME A; PALMITOYLCARNITINE; RATS; RATS, SPRAGUE-DAWLEY;

ANIMALIA; FELIS CATUS;

EID: 1542374565     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.03997.x     Document Type: Article

References (39)

1. McGarry, J.D. & Brown, N.F. (1997) The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis. Eur. J. Biochem. 244, 1-14.
2. Zammit, V.A. (1999) Carnitine acyltransferases: functional significance of subcellular distribution and membrane topology. Prog. Lipid Res. 38, 199-224.
3. Zammit, V.A. (1999) The malonyl-CoA-long-chain acyl-CoA axis in the maintenance of mammalian cell function. Biochem. J. 343, 505-515.
4. Fraser, F. & Zammit, V.A. (1999) Submitochondrial and subcellular distributions of the carnitine-acylcarnitine carrier. FEBS Lett. 445, 41-44.
5. Owen, M.R., Corstorphine, C.C. & Zammit, V.A. (1997) Overt and latent activities of diacylglycerol acytransferase in rat liver microsomes: possible roles in very-low-density lipoprotein triacylglycerol secretion. Biochem. J. 323, 17-21.
6. Waterman, I.J. & Zammit, V.A. (2002) Activities of overt and latent diacylglycerol acyltransferases (DGATs I and II) in liver microsomes of ob/ob mice. Int. J. Obes. Relat. Metab. Disord. 26, 742-743.
7. Waterman, I.J. & Zammit, V.A. (2002) Differential effects of fenofibrate or simvastatin treatment of rats on hepatic microsomal overt and latent diacylglycerol acyltransferase activities. Diabetes 51, 1708-1713.
8. Gibbons, G.F., Bartlett, S.M., Sparks, C.E. & Sparks, J.D. (1992) Extracellular fatty acids are not utilized directly for the synthesis of very-low-density lipoprotein in primary cultures of rat hepatocytes. Biochem. J. 287, 749-753.
9. Gibbons, G.F., Islam, K. & Pease, R.J. (2000) Mobilisation of triacylglycerol stores. Biochim. Biophys. Acta 1483, 37-57.
10. Gibbons, G.F. & Wiggins, D. (1994) Intracellular triacylglycerol lipase: its role in the assembly of hepatic very-low-density lipoprotein (VLDL). Adv. Enzyme Regul. 35, 179-198.
11. Yang, L.Y., Kuksis, A., Myher, J.J. & Steiner, G. (1995) Origin of triacylglycerol moiety of plasma very low density lipoproteins in the rat: structural studies. J. Lipid Res. 36, 125-136.
12. Broadway, N.M., Gooding, J.M. & Saggerson, E.D. (1999) Carnitine acyltransferases and associated transport processes in the endoplasmic reticulum. Missing links in the VLDL story? Adv. Exp Medical Biol. 466, 59-67.
13. Joyce, C., Skinner, K., Anderson, R.A. & Rudel, L.L. (1999) Acyl-coenzyme A: cholesteryl acyltransferase 2. Curr. Opin. Lipidol. 10, 89-95.
14. Rudel, L.L., Lee, R.G. & Cockman, T.L. (2001) Acyl coenzyme A: cholesterol acyltransferase types 1 and 2: structure and function in atherosclerosis. Curr. Opin. Lipidol. 12, 121-127.
15. Buhman, K.F., Accad, M. & Farese, R.V. (2000) Mammalian acyl-CoA: cholesterol acyltransferases. Biochim. Biophys. Acta 1529, 142-154.
16. Joyce, C.W., Shelness, G.S., Davis, M.A., Lee, R.G., Skinner, K., Anderson, R.A. & Rudel, L.L. (2000) ACAT1 and ACAT2 membrane topology segregates a serine residue essential for activity to opposite sides of the endoplasmic reticulum membrane. Mol. Biol. Cell. 11, 3675-3687.
17. Polokoff, M.A. & Bell, R.M. (1978) Limited palmitoyl-CoA penetration into microsomal vesicles as evidenced by a highly latent ethanol acyltransferase activity. J. Biol. Chem. 253, 7173-7178.
18. Murthy, M.S. & Pande, S.V. (1994) Malonyl-CoA-sensitive and -insensitive carnitine palmitoyltransferase activities of microsomes are due to different proteins. J. Biol. Chem. 269, 18283-18286.
19. Broadway, N.M. & Saggerson, E.D. (1995) Solubilization and separation of two distinct carnitine acyltransferases from hepatic microsomes: characterization of the malonyl-CoA-sensitive enzyme. Biochem. J. 310, 989-995.
20. Broadway, N.M., Pease, R.J., Birdsey, G., Shayeghi, M., Turner, N.A. & David Saggerson, E. (2003) The liver isoform of carnitine palmitoyltransferase 1 is not targeted to the endoplasmic reticulum. Biochem. J. 370, 223-231.
21. Fraser, F., Corstorphine, C.G., Price, N.T. & Zammit, V.A. (1999) Evidence that carnitine palmitoyltransferase I (CPT I) is expressed in microsomes and peroxisomes of rat liver. Distinct immunoreactivity of the N-terminal domain of the microsomal protein. FEBS Lett. 446, 69-74.
22. Cohen, I., Guillerault, F., Girard, J. & Prip-Buus, C. (2001) The N-terminal domain of rat liver carnitine palmitoyltransferase 1 contains an internal mitochondrial import signal and residues essential for folding of its C-terminal catalytic domain. J. Biol. Chem. 276, 5403-5411.
23. Prip-Buus, C., Cohen, I., Kohl, C., Esser, V., McGarry, J.D. & Girard, J. (1998) Topological and functional analysis of the rat liver carnitine palmitoyltransferase 1 expressed in Saccharomyces cerevisiae. FEBS Lett. 429, 173-178.
24. Abo-Hashema, K.A., Cake, M.H., Power, G.W. & Clarke, D. (1999) Evidence for triacylglycerol synthesis in the lumen of microsomes via a lipolysis-esterification pathway involving carnitine acyltransferases. J. Biol. Chem. 274, 35577-35582.
25. Diczfalusy, M.A., Bjorkhem, I., Einarsson, C. & Alexson, S.E. (1999) Formation of fatty acid ethyl esters in rat liver microsomes. Evidence for a key role for acyl-CoA: ethanol O-acyltransferase. Eur. J. Biochem. 259, 404-411.
26. Jones, L.R., Maddock, S.W. & Besch, H.R. Jr (1980) Unmasking effect of alamethicin on the (Na+,K+)-ATPase, beta-adrenergic receptor-coupled adenylate cyclase, and cAMP-dependent protein kinase activities of cardiac sarcolemmal vesicles. J. Biol. Chem. 255, 9971-9980.
27. Nagaraj, R. & Balaram, P. (1981) Alamethicin, a transmembrane channel. Acc. Chem. Res. 14, 356-362.
28. Ojcius, D.M. & Young, J.D. (1991) Cytolytic pore-forming proteins and peptides: is there a common structural motif? Trends Biochem. Sci. 16, 225-229.
29. Fulceri, R., Banhegyi, G., Gamberucci, A., Giunti, R., Mandl, J. & Benedetti, A. (1994) Evidence for the intraluminal positioning of p-nitrophenol UDP-glucuronosyltransferase activity in rat liver microsomal vesicles. Arch. Biochem. Biophys. 309, 43-46.
30. Pederson, B.A., Foster, J.D. & Nordlie, R.C. (1998) Low-Km mannose-6-phosphatase as a criterion for microsomal integrity. Biochem. Cell Biol. 76, 115-124.
31. Banhegyi, G., Csala, M., Mandl, J., Burchell, A., Burchell, B., Marcolongo, P., Fulceri, R. & Benedetti, A. (1996) Fatty acyl-CoA esters and the permeability of rat liver microsomal vesicles. Biochem. J. 320, 343-344.
32. Garland, P.B., Shepherd, D. & Yates, D.W. (1965) Steady-state concentrations of coenzyme A, acetyl-coenzyme A and long-chain fatty acyl-Coenzyme A in rat-liver mitochondria oxidizing palmitate. Biochem. J. 97, 587-594.
33. Pande, S.V. & Parvin, R. (1976) Characterization of carnitine acylcarnitine translocase system of heart mitochondria. J. Biol. Chem. 251, 6683-6691.
34. Noel, H., Goswami, T. & Pande, S.V. (1985) Solubilization and reconstitution of rat liver mitochondrial carnitine acylcarnitine translocase. Biochemistry 24, 4504-4509.
35. Murthy, M.S. & Pande, S.V. (1984) Mechanism of carnitine acylcarnitine translocase-catalyzed import of acylcarnitines into mitochondria. J. Biol. Chem. 259, 9082-9089.
36. Indiveri, C., Tonazzi, A. & Palmieri, F. (1991) Characterization of the unidirectional transport of carnitine catalyzed by the reconstituted carnitine carrier from rat liver mitochondria. Biochim. Biophys. Acta 1069, 110-116.
37. Nilsson, R., Peterson, E. & Dallner, G. (1973) Permeability of microsomal membranes isolated from rat liver. J. Cell Biol. 56, 762-776.
38. Ballas, L.M. & Arion, W.J. (1977) Measurement of glucose 6-phosphate penetration into liver microsomes. Confirmation of substrate transport in the glucose-6-phosphatase system, J. Biol. Chem. 252, 8512-8518.
39. Meissner, G. & Allen, R. (1981) Evidence for two types of rat liver microsomes with differing permeability to glucose and other small molecules. J. Biol. Chem. 256, 6413-6422.