Dietary nucleotides modulate mitochondrial function of intestinal mucosa in weanling rats with chronic diarrhea

Journal of Pediatric Gastroenterology and Nutrition

Volumn 37, Issue 2, 2003, Pages 124-131

  Amaud, Alexandra ^a   López Pedrosa, José María ^a   Torres, María Isabel ^b   Gil, Ángel ^b  

^a ABBOTT LABORATORIES   (Spain)

^b UNIVERSITY OF GRANADA   (Spain)

Author keywords

Chronic diarrhea; Dietary nucleotides; Intestine; Mitochondria

Indexed keywords

ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATASE; CITRATE SYNTHASE; CYTIDINE PHOSPHATE; CYTOCHROME C OXIDASE; GUANOSINE PHOSPHATE; INOSINE PHOSPHATE; LACTOSE; MALATE DEHYDROGENASE; NUCLEOTIDE; PROTEIN; URIDINE PHOSPHATE; GLUTAMATE DEHYDROGENASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CARBOHYDRATE INTAKE; CHRONIC DIARRHEA; COLON MUCOSA; CONTROLLED STUDY; DIET; DIET SUPPLEMENTATION; ENERGY METABOLISM; ENZYME ACTIVITY; HISTOPATHOLOGY; ILEUM MUCOSA; INFANT DISEASE; INTESTINE INJURY; INTESTINE MUCOSA; MITOCHONDRIAL RESPIRATION; NEWBORN; NONHUMAN; NUCLEOTIDE TRANSPORT; OXIDATION KINETICS; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN PURIFICATION; RAT; REDUCTION KINETICS; ANIMAL; CHRONIC DISEASE; DIARRHEA; DISEASE MODEL; DRUG EFFECT; ENZYMOLOGY; METABOLISM; MITOCHONDRION; ORAL DRUG ADMINISTRATION; PATHOLOGY; PATHOPHYSIOLOGY; RANDOMIZATION; SMALL INTESTINE; WEANING; WISTAR RAT;

ANIMALIA;

ADENOSINE TRIPHOSPHATASES; ADMINISTRATION, ORAL; ANIMALS; CHRONIC DISEASE; CITRATE (SI)-SYNTHASE; DIARRHEA; DISEASE MODELS, ANIMAL; ELECTRON TRANSPORT COMPLEX IV; GLUTAMATE DEHYDROGENASE; INTESTINAL MUCOSA; INTESTINE, SMALL; MALATE DEHYDROGENASE; MITOCHONDRIA; NUCLEOTIDES; RANDOM ALLOCATION; RATS; RATS, WISTAR; WEANING;

EID: 1542351835     PISSN: 02772116     EISSN: None     Source Type: Journal    
DOI: 10.1097/00005176-200308000-00008     Document Type: Article

References (29)

1. Bueno J, Torres MI, Almendros A, et al. Effect of dietary nucleotides on small intestinal repair after diarrhoea. Histological and ultrastructural changes. Gut 1994;35:926-33.
2. Fagundes-Neto U, Wehba J, Viaro T, et al. Protracted diarrhea in infancy: clinical aspects and ultrastructural analysis of the small intestine. J Pediatr Gastroenterol Nutr 1985;4:714-22.
3. Costa SD, Gomes TA, Haapalainen E, et al. Scanning electronic microscopy of the small intestine in persistent diarrhea. Arq Gastroenterol 1997;34:112-20.
4. Nieto N, López-Pedrosa JM, Mesa MD, et al. Chronic diarrhea impairs intestinal antioxidant defense system in rats at weaning. Dig Dis Sci 2000;45:2044-50.
5. Nuñez MC, Ayudarte MV, Morales D, et al. Effect of dietary nucleotides on intestinal repair in rats with experimental chronic diarrhea. JPEN 1990;14:598-604.
6. Olowookere JO, Olorunsogo OO, Malomo SO. Effects of defective in vivo synthesis of mitochondrial proteins on cellular biochemistry and physiology of malnourished rats. Ann Nutr Metab 1990; 34:147-54.
7. Zakharenko MO. Level and correlation of metabolites of NAD(P)+-dependent dehydrogenase systems in newborn calf tissues in acute diarrhea. Ukr Biokhim Zh 1992;64:39-44.
8. Rudolph FB. The biochemistry and physiology of nucleotides. J Nutr 1994;124:124-7.
9. Uauy R, Stringel G, Thomas R, et al. Effect of dietary nucleosides on growth and maturation of the developing gut in the rat. J Pediatr Gastroenterol Nutr 1990;10:497-503.
10. Ortega MA, Gil A, Sánchez-Pozo A. Maturation status of small intestine epithelium in rats deprived of dietary nucleotides. Life Sci 1995;56:1623-30.
11. Norton R, Leite J, Vieira E, et al. Use of nucleotides in weanling rats with diarrhea induced by a lactose overload: effect on the evolution of diarrhea and weight and on the histopathology of intestine, liver and spleen. Braz J Med Biol Res 2001;2:195-202.
12. Brunser O, Espinoza J, Araya M, et al. Effect of dietary nucleotide supplementation on diarrhoeal disease in infants. Acta Paediatr 1994;83:188-91.
13. American Institute of Nutrition. Report of AIN Ad Hoc Committee on Standards for Nutritional Studies. J Nutr 1977;107:1340-8.
14. Enríquez JA, Pérez-Martos A, López-Pérez MJ, et al. In organello RNA synthesis system from mammalian liver and brain. In: Attardi GM, Chomyn A, eds. Methods Enzymology vol 264. New York: Academic Press, Inc; 1996:50-7.
15. Reynolds ES. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 1963;17:208-12.
16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1996;72:248-54.
17. Zilversmit DB. Determination of total phosphorous and phospholipids. J Lab Clin Med 1950;35:155.
18. Storrie B, Madden EA. Isolation of subcellular organelles. In: Deutscher MP, ed. Methods Enzymology vol 182. New York: Academic Press, Inc; 1990:214-5.
19. Srere PA. Citrate synthase. In: Lowenstein JM, ed. Methods Enzymology New York: Academic Press, Inc; vol 13. 1969:3-11.
20. Kitto GB. Intra- and extramitochondrial malate dehydrogenases from chicken and tuna heart. In: Lowenstein JM, ed. Methods Enzymology vol 13. New York: Academic Press, Inc; 1969:106-8.
21. Fagundes-Neto U, Pontes-Pacheco I, Reis da Silva F, et al. Ultrastructural study of alterations in the small intestinal epithelium of children with acute diarrhea. J Pediatr Gastroenterol Nutr 1984; 3:510-5.
22. Ferreira J, Gil A. Nutritional effects on mitochondrial bioenergetics. Alterations in oxidative phosphorylation by rat liver mitochondria. Biochem J 1984;218:61-7.
23. Olorunsogo OO. Changes in brain mitochondrial bioenergetics in protein-deficient rats. Br J Exp Pathol 1989;6:607-19.
24. Sánchez-Pozo A, Rueda R, Fontana L, et al. Dietary nucleotides and cell growth. Comparative Biochem Physiol 1998;5:99-111.
25. Iijima S, Tsujinaka T, Kido Y, et al. Intravenous administration of nucleosides and a nucleotide mixture diminishes intestinal mucosal atrophy induced by total parenteral nutrition. JPEN 1993;17: 265-70.
26. Arnaud A. Nucleosides modulate the gene expression of hepatic fibrogenesis and functionality markers in cell culture [Ph.D. thesis]. Granada, Spain: University of Granada; 2000.
27. López-Navarro AT, Gil A, Sánchez-Pozo A. Deprivation of dietary nucleotides results in a transient decrease in acid-soluble nucleotides and RNA concentration in rat liver. J Nutr 1995;125:2090-5.
28. Gaal T, Bartlett MS, Ross W, et al. Transcription regulation by initiating NTP concentration: rRNA synthesis in bacteria. Science 1997;278:2073-4.
29. López-Navarro AT, Ortega MA, Peragón J, et al. Deprivation of dietary nucleotides decreases protein synthesis in the liver and small intestine in rats. Gastroenterology 1996;110:1760-9.