Internal transcribed spacer 2 amplicon as a molecular marker for identification of Peronospora parasitica (crucifer downy mildew)

Journal of Applied Microbiology

Volumn 96, Issue 3, 2004, Pages 579-587

  Casimiro, S ^a,b   Moura, M ^a,b   Ze Ze L ^b   Tenreiro, R ^b,c   Monteiro, A A ^a  

^a INSTITUTO SUPERIOR DE AGRONOMIA   (Portugal)

^b UNIVERSITY OF LISBON   (Portugal)

^c UNIVERSITY OF LISBON   (Portugal)

Author keywords

ARDRA; ITS; Molecular identification; Oomycetes; Peronospora parasitica; Peronosporales; rDNA

Indexed keywords

FUNGI; PATHOGENS; PLASTIC CONTAINERS; POLYMERASE CHAIN REACTION; TRANSCRIPTION;

AMPLICONS; ARDRA; DOWNY MILDEW; INTERNAL TRANSCRIBED SPACERS; MOLECULAR IDENTIFICATION; MOLECULAR MARKER; OOMYCETES; PERONOSPORALE; PERONOSPORUM PARASITICUM; RDNA;

DIAGNOSIS;

INTERNAL TRANSCRIBED SPACER 2; RESTRICTION ENDONUCLEASE; RIBOSOME DNA; RIBOSOME RNA;

GENETIC MARKER; MICROBIOLOGY;

AMPLICON; ARTICLE; CONTROLLED STUDY; CRUCIFERA; DNA ISOLATION; DOWNY MILDEW; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; GENE AMPLIFICATION; MOLECULAR BIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PERONOSPORA PARASITICA; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SEQUENCE ANALYSIS;

BRASSICA; BRASSICA OLERACEA; BRASSICACEAE; FUNGI; HYALOPERONOSPORA PARASITICA; OOMYCETES; PERONOSPORA; PERONOSPORA FARINOSA; PERONOSPORA PARASITICA; PERONOSPORALES; TEREBRANTIA;

EID: 1542299157     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2004.02193.x     Document Type: Article

References (18)

1. Altschul, S.F., Medden, T.L., Schäffer, A.A., Zhang, J., Miller, W. and Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389-3402.
2. Badul, J. and Achar, P.N. (1998) Screening of Brassica oleracea var. capitata cultivars against Peronospora parasitica. Seed Science and Technology 26, 385-391.
3. Buscot, F., Wipf, D., Di Battista, C., Munch, J.C., Botton, B. and Martin, F. (1996) DNA polymorphism in morels: PCR/RFLP analysis of the ribosomal DNA spacers and microsatellite-primed PCR. Mycological Research 100, 63-71.
4. Channon, A.G. (1981) Downy mildew of brassicas. In The Downy Mildews ed. Spencer, D.M. pp. 321-339. London: Academic Press.
5. Coelho, P., Leckie, D., Astley, D., Crute, I.R., Bahccevandziev, K., Valério, L., Monteiro, A. and Buokema, I. (1999) The relationship between cotyledon and adult plant resistance to downy mildew (P. parasitica) in B. oleracea. Acta Horticulturae 459, 335-342.
6. Cooke, D.E.L., Drenth, A., Duncan, J.M., Wagels, G. and Brasier, C.M. (2000) A molecular phylogeny of Phytophtora and related Oomycetes. Fungal Genetics and Biology 30, 17-32.
7. Corpet, F. (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research 16, 10881-10890.
8. Dickinson, C.H. and Greenhalgh, J.R. (1977) Host range and taxonomy of Peronospora on crucifers. Transactions of the British Mycological Society 69, 111-116.
9. Duncan, J.M., Cooke, D., Brich, P. and Toth, R. (1998) Molecular variability in sexually reproducing fungal plant pathogens. In Molecular Variability of Fungal Pathogens ed. Bridge, P.D., Couteaudier, Y. and Clarkson, J.M. pp. 19-39. Wallingford: CAB International.
10. FAO (1999) Quarterly Bulletin of Statistics 12, 76-77.
11. Ferreira, M.E. and Grattapaglia, D. (1995) Introdução ao Uso de Marcadores RAPD e RFLP em Análise Genética. Brasilia: EMBRAPA-CENARGEN.
12. Kluczewski, S.M. and Lucas, J.A. (1983) Host infection and oospore formation by P. parasitica in agricultural and horticultural Brassica species. Transactions of the British Mycological Society 81, 591-596.
13. Lanfranco, L., Perotto, S., Longato, S., Mello, A., Cometti, V. and Bonfante, P. (1998) Molecular approaches to investigate biodiversity in mycorrhizal fungi. In Mycorrhiza Manual ed. Varma, A. pp. 353-372. Berlin: Springer-Verlag.
14. Leckie, D., Cogan, N., Astley, D., Crute, I.R., Boukema, I., Santos, M., Bahccevandziev, K., Silva-Dias, J.E. et al. (1999) Differential resistance to P. parasitica and Albugo candida in B. oleracea. Acta Horticulturae 459, 357-361.
15. Mills, P.R., Sreenivasaprasad, S. and Muthumeenakshi, S. (1998) Assessing diversity in Colletotrichum and Trichoderma species using molecular markers. In Molecular Variability of Fungal Pathogens ed. Bridge, P.D., Couteaudier, Y. and Clarkson, J.M. pp. 105-120. Wallingford: CAB International.
16. Monteiro, A. A. and Lunn, T. (1999) Trends and perspectives of vegetable Brassica breeding world-wide. Acta Horticulturae 459, 273-279.
17. Rehmany, A.P., Lynn, J.R., Mahmut, T., Holub, E.B. and Beynon, J. (2000) A comparison of Peronospora parasitica (downy mildew) isolates from Arabidopsis thaliana and Brassica oleracea using Amplified Length Polymorphism and Internal Transcribed Spacer 1 sequence analyses. Fungal Genetics and Biology 30, 95-103.
18. White, T., Brurns, T., Lee, S. and Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications eds Innis, M.A., Gelfand, D.H., Snisky, J.J. and White, T.J. pp. 315-322. San Diego: Academic Press.