Cellular XylS Levels Are a Function of Transcription of xylS from Two Independent Promoters and the Differential Efficiency of Translation of the Two mRNAs

Journal of Bacteriology

Volumn 186, Issue 6, 2004, Pages 1898-1901

  González Pérez, M Mar ^a   Ramos, Juan L ^a   Marqués, Silvia ^a  

^a CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BENZOIC ACID DERIVATIVE; BETA GALACTOSIDASE; MESSENGER RNA; PROTEIN XYLS; TOLUENE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; CONTROLLED STUDY; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS PUTIDA; RNA TRANSLATION; XYLS GENE;

BACTERIAL PROTEINS; BASE SEQUENCE; BENZOATES; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); PROTEIN BIOSYNTHESIS; PSEUDOMONAS PUTIDA; RNA, MESSENGER; TOLUENE; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 1542286907     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.6.1898-1901.2003     Document Type: Article

References (21)

1. de Lorenzo, V., M. Herrero, M. Metzke, and K. N. Timmis. 1991. An upstream XylR and IHF induced nucleoprotein complex regulates the σ54 dependent Pu promotor of TOL plasmid. EMBO J. 10:1159-1167.
2. Fellay, R., J. Frey, and H. Krisch. 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52:147-154.
3. Franklin, F. C. H., M. Bagdasarian, M. M. Bagdasarian, and K. N. Timmis. 1981. Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. Proc. Natl. Acad. Sci. USA 78:7458-7462.
4. 54 -dependent tandem promoters according to the compound used for growth. J. Bacteriol. 178:2356-2361.
5. Holtel, A., M. A. Abril, S. Marqués, K. N. Timmis, and J. L. Ramos. 1990. Promoter upstream activator sequences are required for expression of the xylS gene and upper pathway operon on the Pseudomonas TOL plasmid. Mol. Microbiol. 4:1551-1556.
6. Inouye, S., A. Nakazawa, and T. Nakazawa. 1983. Molecular cloning of regulatory gene xylR and operator promoter regions of the xylABC and xylDEGF operons of the TOL plasmid. J. Bacteriol. 155:1192-1199.
7. Inouye, S., A. Nakazawa, and T. Nakazawa. 1985. Determination of the transcription initiation site and identification of the protein product of the regulatory gene xylR for xyl operons on the TOL plasmid. J. Bacteriol. 163:863-869.
8. Inouye, S., A. Nakazawa, and T. Nakazawa. 1987. Expression of the regulatory gene xylS on the TOL plasmid is positively controlled by the xylR gene product. Proc. Natl. Acad. Sci. USA 84:5182-5186.
9. Macián, F., I. Perez-Roger, and M. E. Armengod. 1994. An improved vector system for constructing transcriptional lacZ fusions: analysis of regulation of the dnaA, dnaN, recF and gyrB genes of Escherichia coli. Gene 145:17-24.
10. Manzanera, M., I. Aranda-Olmedo, J. L. Ramos, and S. Marqués. 2001. Molecular characterization of Pseudomonas putida KT2440 rpoH gene regulation. Microbiology 147:1323-1330.
11. Marqués, S., A. Holtel, K. N. Timmis, and J. L. Ramos. 1994. Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWWO of Pseudomonas putida for metabolism of aromatics. J. Bacteriol. 176:2517-2524.
12. Marqués, S., M. Manzanera, M. M. González-Perez, R. Ruiz, and J. L. Ramos. 1999. Biodegradation, plasmid-encoded catabolic pathways, host factors and cell metabolism. Environ. Microbiol. 1:103-104.
13. Marqués, S., M.-T. Gallegos, M. Manzanera, A. Holtel, K. N. Timmis, and J. L. Ramos. 1998. Activation and repression of transcription at the double tandem divergent promoters for the xylR and xylS genes of the TOL plasmid of Pseudomonas putida. J. Bacteriol. 180:2889-2894.
14. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
15. Perez-Martin, J., and V. de Lorenzo. 1996. ATP binding to the sigma 54-dependent activator XylR triggers a protein multimerization cycle catalyzed by UAS DNA. Cell 86:331-339.
16. Ramos, J. L., S. Marqués, and K. N. Timmis. 1997. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annu. Rev. Microbiol. 51:341-373.
17. Ramos, J. L., N. Mermod, and K. N. Timmis. 1987. Regulatory circuits controlling transcription of TOL plasmid operon encoding meta cleavage pathway for degradation of alkylbenzoates by Pseudomonas. Mol. Microbiol. 1:293-300.
18. Ramos, J. L., A. Stolz, W. Reineke, and K. N. Timmis. 1986. Altered effector specificities in regulators of gene expression: TOL plasmid xylS mutants and their use to engineer expansion of the range of aromatics degraded by bacteria. Proc. Natl. Acad. Sci. USA 83:8467-8471.
19. Silhavy, T. J., and J. R. Beckwith. 1985. Uses of lac fusions for the study of biological problems. Microbiol. Rev. 49:398-418.
20. Spooner, R. A., K. Lindsay, and F. C. H. Franklin. 1986. Genetic, functional and sequence analysis of the xylR and xylS regulatory genes of the TOL plasmid pWWO. J. Gen. Microbiol. 132:1347-1358.
21. Stormo, G. D., T. D. Schneider, and L. M. Gold. 1982. Characterization of translational initiation sites in E. coli. Nucleic Acids Res. 10:2971-2996.