A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing β-galactosidase

Parasitology Research

Volumn 95, Issue 4, 2005, Pages 296-298

  Vega, C ^a   Rolon M ^a   Martinez Fernandez A R ^a   Escario, J A ^a   Gomez Barrio A ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BENZNIDAZOLE; BETA GALACTOSIDASE; GLUCOPYRANOSIDE; NIFURTIMOX;

ABSORPTION; ARTICLE; CORRELATION ANALYSIS; CULTURE MEDIUM; CYTOPLASM; ENZYME ACTIVITY; EPIMASTIGOTE; ESCHERICHIA COLI; LYSIS; MICROSOME; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SCREENING; STANDARDIZATION; TRYPANOSOMA CRUZI;

ANIMALS; BETA-GALACTOSIDASE; CHLOROPHENOLS; GALACTOSIDES; LAC OPERON; NIFURTIMOX; NITROIMIDAZOLES; PARASITIC SENSITIVITY TESTS; SPECTROPHOTOMETRY; TRYPANOCIDAL AGENTS; TRYPANOSOMA CRUZI;

ESCHERICHIA COLI; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 15044353918     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-005-1300-3     Document Type: Article

References (8)

1. Buckner FS, Verlinde CL, La Flamme AC, Van Voorhis WC (1996) Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing beta-galactosidase. Antimicrob Agents Chemother 40:2592-2597
2. Castro JA, Diaz de Toranzo EG (1988) Toxic effects of nifurtimox and benznidazole, two drugs used against American trypanosomiasis (Chagas' disease). Biomed Environ Sci 1:19-33
3. Docampo R, Moreno SN, Stoppani AO, Leon W, Cruz FS, Villalta F, Muniz RF (1981) Mechanism of nifurtimox toxicity in different forms of Trypanosoma cruzi. Biochem Pharmacol 30:1947-1951
4. Gómez-Barrio A, Martinez Díaz RA, Atienza J, Escario JA, De Diego C, Avendaño C (1997) New derivatives of gentian violet as trypanocides: in vitro and in vivo assays on Trypanosoma cruzi. Res Rev Parasitol 57:25-31
5. McFadden DC, Seeber F, Boothroyd JC (1997) Use of Toxoplasma gondii expressing β-galactosidase for colorimetric assessment of drug activity in vitro. Antimicrob Agents Chemother 41:1849-1853
6. Muelas-Serrano S, Nogal-Ruiz JJ, Gomez-Barrio A (2000) Setting of a colorimetric method to determine de viability of Trypanosoma cruzi epimastigotes. Parasitol Res 86:999-1002
7. Traub-Cseko YM, Momen H (1995) On Chagas' disease and leishmaniasis. Parasitol Today 11:315-317
8. World Health Organization (1991) Control of Chagas' disease. World Health Organ Tech Rep Ser 811:1-93